PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0072 [new locus tag: NWMN_RS00410 ]
• pan locus tag^?: SAUPAN000935000
• symbol: NWMN_0072
• pan gene symbol^?: galE
• synonym:
• product: NAD dependent epimerase/dehydratase family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0072 [new locus tag: NWMN_RS00410 ]
• symbol: NWMN_0072
• product: NAD dependent epimerase/dehydratase family protein
• replicon: chromosome
• strand: +
• coordinates: 93566..94525
• length: 960
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5329962 NCBI
• RefSeq: YP_001331107 NCBI
• BioCyc:
• MicrobesOnline: 3705603 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  TTGATAACTGGTGGGGCTGGTTTTATTGGGTCGCATTTAGTAGATGATTTACAACAAGAT
  TATGATGTTTATGTTCTAGATAACTATAGAACAGGTAAACGAGAAAATATTAAAAGTTTG
  GCTGACGATCATGTGTTTGAATTAGATATTCGTGAATATGATGCAGTTGAACAAATCATG
  AAGACATATCAATTTGATTATGTTATTCATTTAGCAGCATTAGTTAGTGTTGCTGAGTCG
  GTTGAGAAACCTATCTTATCTCAAGAAATAAACGTCGTAGCAACATTAAGATTGTTAGAA
  ATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTT
  TATGGTGATCTTCCTGATTTGCCTAAAAGTGATCAATCATTAATCTTACCATTATCACCA
  TATGCAATAGATAAATATTACGGCGAACGGACGACATTAAATTATTGTTCGTTATATAAC
  ATACCAACAGCGGTTGTTAAATTTTTTAATGTATTTGGGCCAAGACAGGATCCTAAGTCA
  CAATATTCAGGTGTGATTTCAAAGATGTTCGATTCATTTGAGCATAACAAGCCATTTACA
  TTTTTTGGTGACGGACTGCAAACTAGAGATTTTGTATATGTATATGATGTTGTTCAATCT
  GTACGCTTAATTATGGAACACAAAGATGCAATTGGACACGGTTATAACATTGGTACAGGC
  ACTTTTACTAATTTATTAGAGGTTTATCGTATTATTGGTGAATTATATGGAAAATCAGTC
  GAGCATGAATTTAAAGAAGCACGAAAAGGAGATATTAAGCATTCTTATGCAGATATTTCT
  AACTTAAAGGCATTAGGATTTGTTCCTAAATATACAGTAGAAACAGGTTTAAAGGATTAC
  TTTAATTTTGAGGTAGATAATATTGAAGAAGTTACAGCTAAAGAAGTGGAAATGTCGTGA
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⊟Protein

⊟General

• locus tag: NWMN_0072 [new locus tag: NWMN_RS00410 ]
• symbol: NWMN_0072
• description: NAD dependent epimerase/dehydratase family protein
• length: 319
• theoretical pI: 4.99868
• theoretical MW: 36429
• GRAVY: -0.213166

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-glucose 4,6-dehydratase (TIGR01181; EC 4.2.1.46; HMM-score: 167.5)
  Metabolism Energy metabolism Sugars UDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 167.1)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides ADP-glyceromanno-heptose 6-epimerase (TIGR02197; EC 5.1.3.20; HMM-score: 151.5)
  and 9 more
  NAD dependent epimerase/dehydratase, LLPSF_EDH_00030 family (TIGR04180; HMM-score: 131.9)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides GDP-mannose 4,6-dehydratase (TIGR01472; EC 4.2.1.47; HMM-score: 64.8)
  Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 46)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides CDP-glucose 4,6-dehydratase (TIGR02622; EC 4.2.1.45; HMM-score: 44.8)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-4-dehydrorhamnose reductase (TIGR01214; EC 1.1.1.133; HMM-score: 42.8)
  hopanoid-associated sugar epimerase (TIGR03466; HMM-score: 41.4)
  thioester reductase domain (TIGR01746; HMM-score: 24.7)
  UDP-N-acetylglucosamine 4,6-dehydratase (inverting) (TIGR03589; EC 4.2.1.115; HMM-score: 17)
  UDP-N-acetylglucosamine 4,6-dehydratase/5-epimerase (TIGR04130; EC 4.2.1.-,5.1.3.-; HMM-score: 16.7)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  NADP_Rossmann (CL0063) Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 206.7)
  GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 204.7)
  and 8 more
  3Beta_HSD; 3-beta hydroxysteroid dehydrogenase/isomerase family (PF01073; HMM-score: 48.9)
  RmlD_sub_bind; RmlD substrate binding domain (PF04321; HMM-score: 43.2)
  NAD_binding_4; Male sterility protein (PF07993; HMM-score: 38.2)
  Polysacc_synt_2; Polysaccharide biosynthesis protein (PF02719; HMM-score: 36.9)
  NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 27.5)
  Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 18.6)
  adh_short; short chain dehydrogenase (PF00106; HMM-score: 14.8)
  KR; KR domain (PF08659; HMM-score: 13)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004884
  • TAT(Tat/SPI): 0.000305
  • LIPO(Sec/SPII): 0.001785
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220284 NCBI
• RefSeq: YP_001331107 NCBI
• UniProt: A0A0H3K6A0 UniProt

⊟Protein sequence

• MITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENIKSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVATLRLLEIIKKYNNHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTLNYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDFVYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARKGDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0072 > NWMN_0073

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications