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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0079 [new locus tag: NWMN_RS00445 ]
  • pan locus tag?: SAUPAN000946000
  • symbol: NWMN_0079
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0079 [new locus tag: NWMN_RS00445 ]
  • symbol: NWMN_0079
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 101347..102102
  • length: 756
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGAGGTTGTCTACGTGACGCAAACACAACCAAAATTTTTAACGATTTATAATACGTTA
    TATAAAGAAATTCAAATAGGAAAATATCCGAGTGGCCATGCATTACCTACTGAAAAGGAA
    CTTTGTGCGCGCTTTGATGTCAGTCGTATGACTTTGCGCCAAGCTATAAAATTGTTAGCC
    GAAGATGGCATCGTAGAGAGTACAAGAGGGAAGGGACACTTTGTTATTCCACAAACAAAT
    GCCCAACACGCTTCAAGTATGGATCAATTTAAGCATCCACTTGATCAAATATTGCTAGCT
    CGCGTATCTATGTCATCAATTAATTATCGTGTTGATTTAGAAAGTGAATATACAAATCAT
    TTGTTTGCAAACCATCCTTCTGCCGTCATTGCAATGGAACGCTATTATCAAAAAAAGGAC
    AATCATTCTAAGCAAGCTGATGCATTTTGCTTTACCTTTATACCGTTAAATGTCATTGAC
    ACATTTAAAGTGAATACACAAAACGAAGACGACATGAAGACATTCGTTGAGCAAACGATT
    TATAGCAATGCATATCAATCAGATTTAAAAATGTCTATTACCAAAGCGCCCCACTTCAAA
    AATCATAGTCACGTGTTTGATGGTGATACACATTGTTGGCTGATTATTGAAACACTGTAC
    GCACAAACACCTTATCCAATTATGATTAATAAGTGGTATATTCCACAGGAAATATCTGAA
    TTAACACTGACACGTATAAGACAATCGGATTATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    756

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0079 [new locus tag: NWMN_RS00445 ]
  • symbol: NWMN_0079
  • description: hypothetical protein
  • length: 251
  • theoretical pI: 6.9119
  • theoretical MW: 29171
  • GRAVY: -0.41992

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02404; HMM-score: 68.1)
    Metabolism Transport and binding proteins Anions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 55.9)
    Signal transduction Regulatory functions DNA interactions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 55.9)
    Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 55)
    Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 55)
    and 7 more
    Signal transduction Regulatory functions DNA interactions phosphonate utilization transcriptional regulator PhnR (TIGR03337; HMM-score: 42)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 35.8)
    Metabolism Fatty acid and phospholipid metabolism Degradation fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 35.8)
    Signal transduction Regulatory functions DNA interactions fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 35.8)
    phosphonate utilization associated transcriptional regulator (TIGR03338; HMM-score: 24.7)
    Unknown function General Rrf2 family protein (TIGR00738; HMM-score: 14.9)
    Signal transduction Regulatory functions DNA interactions biotin operon repressor (TIGR00122; HMM-score: 12.7)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    HTH (CL0123) GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 62.7)
    and 12 more
    HTH_11; HTH domain (PF08279; HMM-score: 29.2)
    Rrf2; Transcriptional regulator (PF02082; HMM-score: 21.6)
    HTH_20; Helix-turn-helix domain (PF12840; HMM-score: 20.8)
    HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 20)
    HTH_41; Helix-turn-helix domain (PF14502; HMM-score: 17)
    MarR_2; MarR family (PF12802; HMM-score: 16.5)
    HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 13.9)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 13.8)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 13.7)
    no clan defined PASTA; PASTA domain (PF03793; HMM-score: 13.4)
    HTH (CL0123) HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 13.2)
    HTH_29; Winged helix-turn helix (PF13551; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00499
    • TAT(Tat/SPI): 0.000149
    • LIPO(Sec/SPII): 0.000385
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCARFDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARVSMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVIDTFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIETLYAQTPYPIMINKWYIPQEISELTLTRIRQSDY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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