PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0297 [new locus tag: NWMN_RS01695 ]
• pan locus tag^?: SAUPAN000613000
• symbol: NWMN_0297
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0297 [new locus tag: NWMN_RS01695 ]
• symbol: NWMN_0297
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 344771..345220
• length: 450
• essential: unknown

⊟Accession numbers

• Gene ID: 5330136 NCBI
• RefSeq: YP_001331331 NCBI
• BioCyc:
• MicrobesOnline: 3705828 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAATTAAACTACTACGACTTAAGCAGGTGCCATATGGCAAAAGTTAAGTACGGTGCT
  GATAGCATGGTTGTTGAATTGGATAAGTTCGATAAGAAAATAGAAGAGTGGGTTAAAAAA
  GGTATTGCTAAAACAACGACGAAGATTTACAACACTGCTGTAGCATTAGCTCCTGTTGAC
  TTAGGTTTTTTAGAAGAAAGTATTGACTTTAAATATTTCGATGGTGGGTTATCCAGTGTT
  ATAAGTGTCGGCGCAGATTATGCAATATACGTTGAATACGGTACTGGTATATATGCTACT
  GGTCCTGGTGGTAGTCGTGCTACAAAGATTCCGTGGAGTTTTAAAGGTGATGACGGCGAA
  TGGTACACAACATATGGTCAAGCGCCACAGCCATTTTGGAACCCTGCAATTGACGCAGGA
  CGCAAGACATTCGAGCAGTATTTTTCATAG
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⊟Protein

⊟General

• locus tag: NWMN_0297 [new locus tag: NWMN_RS01695 ]
• symbol: NWMN_0297
• description: hypothetical protein
• length: 149
• theoretical pI: 5.23592
• theoretical MW: 16680.7
• GRAVY: -0.328188

⊟Function

• TIGRFAM:
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage protein, HK97 gp10 family (TIGR01725; HMM-score: 39.2)
• TheSEED:
• PFAM:
  Phage_tail (CL0348) HK97-gp10_like; Bacteriophage HK97-gp10, putative tail-component (PF04883; HMM-score: 28.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002436
  • TAT(Tat/SPI): 0.000271
  • LIPO(Sec/SPII): 0.000478
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220509 NCBI
• RefSeq: YP_001331331 NCBI
• UniProt: A0A0H3K6T0 UniProt

⊟Protein sequence

• MKLNYYDLSRCHMAKVKYGADSMVVELDKFDKKIEEWVKKGIAKTTTKIYNTAVALAPVDLGFLEESIDFKYFDGGLSSVISVGADYAIYVEYGTGIYATGPGGSRATKIPWSFKGDDGEWYTTYGQAPQPFWNPAIDAGRKTFEQYFS

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0295 > NWMN_0296 > NWMN_0297 > NWMN_0298 > NWMN_0299 > NWMN_0300

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications