PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0305 [new locus tag: NWMN_RS01735 ]
• pan locus tag^?: SAUPAN000621000
• symbol: NWMN_0305
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0305 [new locus tag: NWMN_RS01735 ]
• symbol: NWMN_0305
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 353131..355029
• length: 1899
• essential: unknown

⊟Accession numbers

• Gene ID: 5330144 NCBI
• RefSeq: YP_001331339 NCBI
• BioCyc:
• MicrobesOnline: 3705836 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGCTTACGGATTAATTACAAGTTTACATTCAATGACAGGTCGGAAAATAGTTGCTCAA
  CATGAGTATAACTATCGCTTGTTAGATGAAGGTATGAGCAAACTTGAGAAAATGTTTATA
  TACCATCAAAAAGAAGAAATATACGCACACTCAGCGAAACAAATTAAATACTTGAATGAC
  AGTGTTGAAGATTATTTAACGTATTTAAATGGCCGTTTTAGCAATATGATTCTAGGCCAT
  AACGGCGACGGTATCAATGAAGTAAAAGACGCGCGTATTGATAATACAGGTTATGGTCAT
  AAGACATTGCAAGATCGTTTGTATCATGATTATTCAACACTAGATGCTTTCACTAAAAAG
  GTTGAGAAAGCTGTAGATGAACACTATAAAGAATATCGAGCGACAGAATACCGATTCGAA
  CCAAAAGAGCAAGAACCGGAATTTATCACTGATTTATCGCCATATACAAATGCAGTAATG
  CAATCATTTTGGGTAGACCCTAGAACGAAAATTATTTATATGACGCAAGCTCGTCCAGGT
  AATCATTACATGTTATCTAGATTGAAGCCCAACGGACAATTTATTGATAGATTGCTTGTT
  AAAAACGGCGGTCACGGTACACACAATGCGTATAGATACATTGATGGAGAATTATGGATT
  TATTCAGCTGTATTGGACAGTAACAAAAACAACAAGTTTGTACGTTTCCAATATAGAACT
  GGAGAAATAACTTATGGTAATGAAATGCAAGATGTCATGCCGAATATATTTAACGACAGA
  TATACGTCAGCGATTTATAATCCGGTAGAAAATTTAATGATTTTTAGACGTGAATATAAA
  CCCACTGAAAGACAACTTAAGAATTCGTTGAACTTTGTTGAGGTTAGAAGTGCTGACGAT
  ATTGATAAAGGTATAGACAAAGTATTGTATCAAATGGATATACCTATGGAATACACTTCA
  GATACACAACCTATGCAAGGTATCACTTATGATGCAGGTATCTTATATTGGTATACAGGT
  GATTCGAATACAGCCAACCCTAACTACTTACAAGGTTTCGATATAAAAACAAAAGAATTG
  TTATTTAAACGACGTATCGATATTGGCGGTGTGAATAATAACTTTAAAGGAGACTTCCAA
  GAAGCTGAGGGTCTAGATATGTATTACGATCTAGAAACAGGACGCAAAGCGCTTTTAATA
  GGGGTAACTATTGGACCTGGTAACAACAGACATCACTCAATTTATTCCATCGGCCAAAGA
  GGTGTTAACCAATTCTTAAAAAACATTGCACCTCAAGTATCGATGACTGATTCAGGCGGA
  CGTGTTAAACCGTTACCAATACAGAACCCAGCATATCTAAGTGATATTACGGAAGTTGGT
  CATTACTATATCTATACGCAAGACACACAAAATGCGTTAGATTTCCCGTTACCGAAAGCG
  TTTAGAGATGCAGGTTGGTTCTTTGATGTACTGCCTGGACACTATAATGGTGCTCTAAGA
  CAAGTACTTACCAGAAACAGCACAGGTAGAAATATGCTTAAATTCGAACGTGTCATTGAC
  ATTTTCAATAAGAAAAACAACGGAGCATGGAATTTCTGTCCGCAAAACGCCGGTTATTGG
  GAACATATCCCTAAGAATATTACAAAATTATCAGATTTAAAAATCGTTGGTTTAGATTTC
  TATATCACTACTGAAGAATCAAAACGATTTACTGATTTTCCTAAAGACTTTAAAGGTATT
  GCAGGTTGGATATTAGAAGTAAAATCGAATACACCGGGTAACACAACACAAGTATTAAGA
  CGTAATAACTTCCCGTCTGCACATCAATTTTTAGTTAGAAACTTTGGTACTGGTGGCGTT
  GGTAAATGGAGTTTATTCGAGGGAAAGGTGGTTGAATAA
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⊟Protein

⊟General

• locus tag: NWMN_0305 [new locus tag: NWMN_RS01735 ]
• symbol: NWMN_0305
• description: hypothetical protein
• length: 632
• theoretical pI: 7.89395
• theoretical MW: 73107.9
• GRAVY: -0.623101

⊟Function

• TIGRFAM:
• TheSEED:
• PFAM:

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005958
  • TAT(Tat/SPI): 0.000209
  • LIPO(Sec/SPII): 0.001328
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220517 NCBI
• RefSeq: YP_001331339 NCBI
• UniProt: A0A0H3KE70 UniProt

⊟Protein sequence

• MAYGLITSLHSMTGRKIVAQHEYNYRLLDEGMSKLEKMFIYHQKEEIYAHSAKQIKYLNDSVEDYLTYLNGRFSNMILGHNGDGINEVKDARIDNTGYGHKTLQDRLYHDYSTLDAFTKKVEKAVDEHYKEYRATEYRFEPKEQEPEFITDLSPYTNAVMQSFWVDPRTKIIYMTQARPGNHYMLSRLKPNGQFIDRLLVKNGGHGTHNAYRYIDGELWIYSAVLDSNKNNKFVRFQYRTGEITYGNEMQDVMPNIFNDRYTSAIYNPVENLMIFRREYKPTERQLKNSLNFVEVRSADDIDKGIDKVLYQMDIPMEYTSDTQPMQGITYDAGILYWYTGDSNTANPNYLQGFDIKTKELLFKRRIDIGGVNNNFKGDFQEAEGLDMYYDLETGRKALLIGVTIGPGNNRHHSIYSIGQRGVNQFLKNIAPQVSMTDSGGRVKPLPIQNPAYLSDITEVGHYYIYTQDTQNALDFPLPKAFRDAGWFFDVLPGHYNGALRQVLTRNSTGRNMLKFERVIDIFNKKNNGAWNFCPQNAGYWEHIPKNITKLSDLKIVGLDFYITTEESKRFTDFPKDFKGIAGWILEVKSNTPGNTTQVLRRNNFPSAHQFLVRNFGTGGVGKWSLFEGKVVE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0301 > NWMN_0302 > NWMN_0303 > NWMN_0304 > NWMN_0305 > NWMN_0306 > NWMN_0307

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications