COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0306 [new locus tag: NWMN_RS01740 ]
• pan locus tag^?:
• symbol: NWMN_0306
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0306 [new locus tag: NWMN_RS01740 ]
• symbol: NWMN_0306
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 355029..356852
• length: 1824
• essential: unknown

⊟Accession numbers

• Gene ID: 5330145 NCBI
• RefSeq: YP_001331340 NCBI
• BioCyc:
• MicrobesOnline: 3705837 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
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  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
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  1021
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  1141
  1201
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  1321
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  1441
  1501
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  1621
  1681
  1741
  1801
  
  ATGATAGTAGATAATTTTTCGAAAGACGATAACTTAATCGAGTTACAAACAACATCACAA
  TATAATCCAATTATTGACACAAACATCAGTTTCTATGAATCAGATAGAGGAACTGGTGTT
  TTAAATTTTGCAGTAACTAAGAATAACAGACCGTTATCTATAAGTTCTGAACATGTTAAG
  ACATCTATCGTGTTAAAAACCGATGATTATAACGTAGATAGAGGCGCTTATATTTCAGAC
  GAATTAACGATAGTAGACGCAATTAATGGGCGTTTGCAGTATGTGATACCGAATGAATTT
  TTAAAACATTCAGGCAAGGTGCATGCTCAGGCATTCTTTACACAAAACGGGAGTAATAAT
  GTTGTTGTTGAACGTCAATTTAGCTTCAATATTGAAAATGATTTAGTTAGTGGGTTTGAT
  GGTATAACAAAGCTTGTTTATATCAAATCTATTCAAGATACTATCGAAGCTGTCGGTAAA
  GACTTTAACCAATTAAAGCAAAATATGGCTGATACACAAACGTTAATAGCAAAAGTGAAT
  GATAGTGCGACAAAAGGCATTCAACAAATCGAAATCAAGCAAAACGAAGCTATACAAGCT
  ATTACTGCGACGCAAACTAGTGCAACACAAGCTGTTACAGCTGAAGTCGATAAAATAGTT
  GAAAAAGAGCAAGCGATTTTTGAACGTGTTAACGAAGTTGAACAACAAATCAATGGCGCT
  GACCTTGTTAAAGGTAATTCAACAACAAATTGGCAAAAGTCTAAACTTACAGATGATTAC
  GGTAAAGCAATTGAATCGTATGAGCAGTCCATAGATAGCGTTTTAAGCGCAGTTAACACA
  TCTAGGATTATTCATATTACTAATGCAACAGATGCGCCAGAAAAGACGGATATAGGCACG
  TTAGAGAAGCCTGGACAAGATGGTGTTGATGACGGTTCTTCGTTCGATGAATCAACTTAT
  ACATCAAGCAAATCTGGTGTGTTAGTTGTTTATGTTGTTGATAATAATACTGCTCGTGCA
  ACATGGTACCCAGACGATTCAAACGATGAGTACACAAAATACAAAATCTACGGCACATGG
  TACCCGTTTTATAAAAAGAATGATGGAAACTTAACTAAGCAATTTGTTGAAGAAACGTCT
  AACAACGCTTTAAATCAAGCTAAGCAGTATGTAGATGATAAATTCGGAACAACGAGCTGG
  CAACAACATAAGATGACAGAGGCGAATGGTCAATCAATTCAAGTTAACTTAAATAATGCG
  CAAGGCGATTTGGGATATTTAACTGCTGGTAATTACTATGCAACAAGAGTGCCGGATTTA
  CCAGGTAGCGTTGAAAGTTATGAGGGTTATTTATCGGTATTCGTTAAAGATGATACAAAC
  AAGCTATTTAACTTCACACCTTATAACTCTAAAAAGATTTACACACGATCAATCACAAAC
  GGCAGACTTGAGCAACAGTGGACAGTTCCTAATGAACATAAATCAACGGTATTGTTCGAC
  GGTGGCGCAAATGGTGTAGGTACAACAATCAATCTAACTGAACCGTACACAAACTATTCT
  ATTTTGTTGGTAAGTGGAACTTATCCAGGTGGCGTTATTGAGGGATTCGGACTAACCGCA
  TTACCTAACGCGATTCAATTGAGTAAAGCGAATGTAGTTGACTCAGACGGCAACGGTGGC
  GGTATTTATGAGTGCTTACTATCCAAAACAAGTAGCACTACTTTAAGAATAGATAACGAT
  GTGTACTTTGATTTAGGTAAAACATCAGGTTCTGGAGCGAATGCCAACAAAGTTACTATA
  ACTAAAATTATGGGGTGGAAATAA
  60
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  1260
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  1440
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  1560
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  1740
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  1824
  

⊟Protein

⊟General

• locus tag: NWMN_0306 [new locus tag: NWMN_RS01740 ]
• symbol: NWMN_0306
• description: hypothetical protein
• length: 607
• theoretical pI: 4.53212
• theoretical MW: 66966.4
• GRAVY: -0.494069

⊟Function

• TIGRFAM:
• TheSEED:
• PFAM:
  no clan defined DUF2479; Domain of unknown function (DUF2479) (PF10651; HMM-score: 122)
  and 4 more
  SNARE-fusion (CL0445) Syntaxin_2; Syntaxin-like protein (PF14523; HMM-score: 15.7)
  no clan defined DUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 14.5)
  Collagen_mid; Bacterial collagen, middle region (PF15984; HMM-score: 11.7)
  GerPC; Spore germination protein GerPC (PF10737; HMM-score: 11.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005932
  • TAT(Tat/SPI): 0.000669
  • LIPO(Sec/SPII): 0.001404
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220518 NCBI
• RefSeq: YP_001331340 NCBI
• UniProt: A0A0H3K649 UniProt

⊟Protein sequence

• MIVDNFSKDDNLIELQTTSQYNPIIDTNISFYESDRGTGVLNFAVTKNNRPLSISSEHVKTSIVLKTDDYNVDRGAYISDELTIVDAINGRLQYVIPNEFLKHSGKVHAQAFFTQNGSNNVVVERQFSFNIENDLVSGFDGITKLVYIKSIQDTIEAVGKDFNQLKQNMADTQTLIAKVNDSATKGIQQIEIKQNEAIQAITATQTSATQAVTAEVDKIVEKEQAIFERVNEVEQQINGADLVKGNSTTNWQKSKLTDDYGKAIESYEQSIDSVLSAVNTSRIIHITNATDAPEKTDIGTLEKPGQDGVDDGSSFDESTYTSSKSGVLVVYVVDNNTARATWYPDDSNDEYTKYKIYGTWYPFYKKNDGNLTKQFVEETSNNALNQAKQYVDDKFGTTSWQQHKMTEANGQSIQVNLNNAQGDLGYLTAGNYYATRVPDLPGSVESYEGYLSVFVKDDTNKLFNFTPYNSKKIYTRSITNGRLEQQWTVPNEHKSTVLFDGGANGVGTTINLTEPYTNYSILLVSGTYPGGVIEGFGLTALPNAIQLSKANVVDSDGNGGGIYECLLSKTSSTTLRIDNDVYFDLGKTSGSGANANKVTITKIMGWK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0301 > NWMN_0302 > NWMN_0303 > NWMN_0304 > NWMN_0305 > NWMN_0306 > NWMN_0307

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications