PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0418 [new locus tag: NWMN_RS02360 ]
• pan locus tag^?: SAUPAN002163000
• symbol: ndhF
• pan gene symbol^?: mpsA
• synonym:
• product: NADH dehydrogenase subunit 5

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0418 [new locus tag: NWMN_RS02360 ]
• symbol: ndhF
• product: NADH dehydrogenase subunit 5
• replicon: chromosome
• strand: +
• coordinates: 467178..468662
• length: 1485
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331831 NCBI
• RefSeq: YP_001331452 NCBI
• BioCyc:
• MicrobesOnline: 3705949 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  1441
  
  GTGTTAAGTTTTCAATTGCTATTTTCACTGTTTGTTATTGCGCTTATCATTGCATTGATA
  AGTGGCTTGTTGTTTTTAGCACCAGTTATGCCAATGAGATATATTAAATTACATTTATAC
  ATACTAGTCATGCCAGTATTATTTGCAGTCATTGGCTTTTTCGGTATTCATGGTCAACAT
  GTCTTAGGTCCATTTAAAATAGATCGTTTATCTTGGTTATTAGCTGGCTTTGTAATGGCG
  CTTGGCTTTATTATTCAAAAGTTTTCAATGCGATATTTACTAGGTGATCATCATTATAGA
  CATTACTTTCCATTGTTCACTGCGATTACGTCGTTTGCATCTTTAGCATGGATGTCTGAA
  GACTTAAGACTGATGGCACTCTGCTGGGGTATTACATTATTATGTTTAACATTGCTGATG
  AACGTTAATCGTTTTTGGAAAGTGCCACGTGAGTCTGCGAAATTATCAAGCATGACATTT
  TTATGTGGTTGGCTTGCATTCGTTGGAGCAATTGTAACTATTTATATTGCGACTGGCGAG
  TGGCGGGTACCACAACATATAGTTCATCCGACATGGTCATTGTTGACGAATGTACTACTT
  GTATTAGCTGTCATGATACCGGCAGCACAATTTCCTTTTCATCGATGGTTGATTGAATCT
  GTAACGGCACCAACGCCAGTATCGGCAATTATGCATGCAGGAATTGTGAATGCAGGTGGT
  GTTATTCTAACTCGTTTTGCGCCGATATTTGATAATGGATTTGCGTTATCATTATTACTT
  ATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGGTATTAGCTTAGTTCAAGTTGACTAT
  AAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCA
  TTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACA
  TTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT
  AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTA
  TTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGG
  TCATTACTTGTATCTTGGAATCAAATGGTAGCCTTTAGTAAAGGACGCATGGCACGTCTT
  GTTGGTATGATTTTGATTGCAATCGTGACATTTATCTACGTCATCACACATAATTATTTT
  TACGCTGTATTACAAAATATAACAACACATGCGACAACGCCGCCTACAGTGAGTGTCATC
  ATTAGTGTTGTTATTTTAATCTTTGGCAGTTTATTAAGTATTTGGGTGGCGCGGCATCGA
  TACTCTAAGGCTTTTGCGGTATTGTACGTGTGGTTAGTTAATCTAGGTGAAGCACGCTCG
  AAAGCGATAGAAAGTCATCCGAATTATTTGAAGAAGTATTTATAG
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⊟Protein

⊟General

• locus tag: NWMN_0418 [new locus tag: NWMN_RS02360 ]
• symbol: NdhF
• description: NADH dehydrogenase subunit 5
• length: 494
• theoretical pI: 10.3582
• theoretical MW: 55256.2
• GRAVY: 0.917409

⊟Function

• TIGRFAM:
  Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain L (TIGR01974; EC 1.6.-.-; HMM-score: 128.8)
  and 4 more
  Metabolism Transport and binding proteins Cations and iron carrying compounds monovalent cation:proton antiporter (TIGR00940; HMM-score: 88.7)
  NAD(P)H dehydrogenase, subunit NdhF3 family (TIGR01960; HMM-score: 55.9)
  Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain M (TIGR01972; HMM-score: 50.2)
  Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain N (TIGR01770; HMM-score: 27.2)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  ComplexI-N (CL0425) Proton_antipo_M; Proton-conducting membrane transporter (PF00361; HMM-score: 175.7)
  and 1 more
  no clan defined Proton_antipo_N; NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus (PF00662; HMM-score: 22.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 14
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004434
  • TAT(Tat/SPI): 0.00094
  • LIPO(Sec/SPII): 0.018181
• predicted transmembrane helices (TMHMM): 14

⊟Accession numbers

• GI: 151220630 NCBI
• RefSeq: YP_001331452 NCBI
• UniProt: A0A0H3KAY3 UniProt

⊟Protein sequence

• MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVMPVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHYFPLFTAITSFASLAWMSEDLRLMALCWGITLLCLTLLMNVNRFWKVPRESAKLSSMTFLCGWLAFVGAIVTIYIATGEWRVPQHIVHPTWSLLTNVLLVLAVMIPAAQFPFHRWLIESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLVQVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNIPKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILGWSLLVSWNQMVAFSKGRMARLVGMILIAIVTFIYVITHNYFYAVLQNITTHATTPPTVSVIISVVILIFGSLLSIWVARHRYSKAFAVLYVWLVNLGEARSKAIESHPNYLKKYL

⊟Experimental data

• experimentally validated:
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: ndhF > NWMN_0419

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications