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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00412
  • pan locus tag?: SAUPAN002163000
  • symbol: SAOUHSC_00412
  • pan gene symbol?: mpsA
  • synonym:
  • product: NADH dehydrogenase subunit 5

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00412
  • symbol: SAOUHSC_00412
  • product: NADH dehydrogenase subunit 5
  • replicon: chromosome
  • strand: +
  • coordinates: 413512..414996
  • length: 1485
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    GTGTTAAGTTTTCAATTGCTATTTTCACTGTTTGTTATTGCGCTTATCATTGCATTGATA
    AGTGGCTTGTTGTTTTTAGCACCAGTTATGCCAATGAGATATATTAAATTACATTTATAC
    ATACTAGTCATGCCAGTATTATTTGCAGTCATTGGCTTTTTCGGTATTCATGGTCAACAT
    GTCTTAGGTCCATTTAAAATAGATCGTTTATCTTGGTTATTAGCTGGCTTTGTAATGGCG
    CTTGGCTTTATTATTCAAAAGTTTTCAATGCGATATTTACTAGGTGATCATCATTATAGA
    CATTACTTTCCATTGTTCACTGCGATTACGTCGTTTGCATCTTTAGCATGGATGTCTGAA
    GACTTAAGACTGATGGCACTCTGCTGGGGTATTACATTATTATGTTTAACATTGCTGATG
    AACGTTAATCGTTTTTGGAAAGTGCCACGTGAGTCTGCGAAATTATCAAGCATGACATTT
    TTATGTGGTTGGCTTGCATTCGTTGGAGCAATTGTAACTATTTATATTGCGACTGGCGAG
    TGGCGGGTACCACAACATATAGTTCATCCGACATGGTCATTGTTGACGAATGTACTACTT
    GTATTAGCTGTCATGATACCGGCAGCACAATTTCCTTTTCATCGATGGTTGATTGAATCT
    GTAACGGCACCAACGCCAGTATCGGCAATTATGCATGCAGGAATTGTGAATGCAGGTGGT
    GTTATTCTAACTCGTTTTGCGCCGATATTTGATAATGGATTTGCGTTATCATTATTACTT
    ATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGGTATTAGCTTAGTTCAAGTTGACTAT
    AAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCA
    TTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACA
    TTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT
    AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTA
    TTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGG
    TCATTACTTGTATCTTGGAATCAAATGGTAGCCTTTAGTAAAGGACGCATGGCACGTCTT
    GTTGGTATGATTTTGATTGCAATCGTGACATTTATCTACGTCATCACACATAATTATTTT
    TACGCTGTATTACAAAATATAACAACACATGCGACAACGCCGCCTACAGTGAGTGTCATC
    ATTAGTGTTGTTATTTTAATCTTTGGCAGTTTATTAAGTATTTGGGTGGCGCGGCATCGA
    TACTCTAAGGCTTTTGCGGTATTGTACGTGTGGTTAGTTAATCTAGGTGAAGCACGCTCG
    AAAGCGATAGAAAGTCATCCGAATTATTTGAAGAAGTATTTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1485

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00412
  • symbol: SAOUHSC_00412
  • description: NADH dehydrogenase subunit 5
  • length: 494
  • theoretical pI: 10.3582
  • theoretical MW: 55256.2
  • GRAVY: 0.917409

⊟Function[edit | edit source]

  • reaction:
    EC 1.6.5.3?  ExPASy
    NADH:ubiquinone reductase (H+-translocating) NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out)
  • TIGRFAM:
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain L (TIGR01974; EC 1.6.-.-; HMM-score: 128.8)
    and 4 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds monovalent cation:proton antiporter (TIGR00940; HMM-score: 88.7)
    NAD(P)H dehydrogenase, subunit NdhF3 family (TIGR01960; HMM-score: 55.9)
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain M (TIGR01972; HMM-score: 50.2)
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain N (TIGR01770; HMM-score: 27.2)
  • TheSEED  :
    • NADH dehydrogenase, subunit 5
  • PFAM:
    ComplexI-N (CL0425) Proton_antipo_M; Proton-conducting membrane transporter (PF00361; HMM-score: 175.7)
    and 1 more
    no clan defined Proton_antipo_N; NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus (PF00662; HMM-score: 22.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004434
    • TAT(Tat/SPI): 0.00094
    • LIPO(Sec/SPII): 0.018181
  • predicted transmembrane helices (TMHMM): 14

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVMPVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHYFPLFTAITSFASLAWMSEDLRLMALCWGITLLCLTLLMNVNRFWKVPRESAKLSSMTFLCGWLAFVGAIVTIYIATGEWRVPQHIVHPTWSLLTNVLLVLAVMIPAAQFPFHRWLIESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLVQVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNIPKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILGWSLLVSWNQMVAFSKGRMARLVGMILIAIVTFIYVITHNYFYAVLQNITTHATTPPTVSVIISVVILIFGSLLSIWVARHRYSKAFAVLYVWLVNLGEARSKAIESHPNYLKKYL

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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