From AureoWiki
Jump to navigation Jump to search

NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0511 [new locus tag: NWMN_RS02970 ]
  • pan locus tag?: SAUPAN002321000
  • symbol: NWMN_0511
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0511 [new locus tag: NWMN_RS02970 ]
  • symbol: NWMN_0511
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 588713..589897
  • length: 1185
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    GTGATAAGGGTGTTAGATTGGTTCCAACTAGCAAATAATAAAGAAAATAAAACAATTCAA
    CTCAGACGTTATTTACACCAATATCCTGAGTTATCTTTTGAAGAATTTCAAACACATGAT
    TATATTGTTAACCAATTAAGCCAGTTATCTTGTGATATTGAAACTCCTATCGGCCGCAAT
    GGTATCAAAGCCACATTTAAAGGCTTGGGCACAGGACCTACCATTGCATTAAGAGCTGAC
    TTTGATGCGTTACCTGTCGAGGAATTAAACGATGTGCCTTATAAGTCTAAAAATCCAGGA
    TGTATGCATGCGTGTGGGCATGATGGTCATACAGCAATTTTACTTACTGTAGCAGAGATT
    TTAGATGAACATAAACATTTATTAGAAGGTAATGTTGTATTAATATTCCAGTACGGTGAA
    GAAATCATGCCAGGTGGTTCTCAAGAAATGATAGATGCTGGATGTCTGGAAAATGTAGAT
    AGAATTTACGGTACACATTTATGGAGTGGTTATCCTACTGGAACTATTCATTCACGTGCG
    GGGGCAATCATGGCCTCTCCAGATGAATTTAGCGTCACATTTAAAGGTCGTGGTGGTCAT
    GGTGCCAAACCTCATGAAACCATTGACCCAATAGTAATCATGGCAGAGTTCATTTTAAGT
    GCACAAAAAATAATTTCACGTACGATCGATCCAGTTAAACAAGCTGTACTTTCATTTGGA
    ATGATTCAAGCAGGCACCACAGATAGCGTTATTCCTGATCAAGCATTTTGTAAAGGTACA
    GTTAGAACTTTCGATTCAGATATACAGAATCATGTAATGGACAAAATGGATAAGCTTCTA
    CAAGGTTTAGCAATAGCAAATGATATTAACTATGATTTGAATTATATTAAAGGGTATTTG
    CCTGTCCATAATAACGAGAAAGCATATCAAGTTATTAAAGAAGCAACAAATGACTTACAT
    GTACGCTTTAATGAATCAGATTTAATGATGATTGGAGAAGATTTTTCACATTATTTGAAA
    GTACGACCAGGTGCATTCTTTCTGACTGGTTGTGGTAATGAAAGTAAAGGTATTACAGCG
    CCACACCATAATCCCAAATTTGATATTGATGAAAAATCATTAAAATACGCTGTAGCTGTA
    TTTTTAAAAATCATAGAATTAGAGCAAGTATTTAAAACAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1185

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_0511 [new locus tag: NWMN_RS02970 ]
  • symbol: NWMN_0511
  • description: hypothetical protein
  • length: 394
  • theoretical pI: 5.79675
  • theoretical MW: 44025.9
  • GRAVY: -0.223096

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides amidohydrolase (TIGR01891; HMM-score: 385.9)
    and 6 more
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 38)
    Metabolism Amino acid biosynthesis Glutamate family acetylornithine deacetylase (ArgE) (TIGR01892; EC 3.5.1.16; HMM-score: 30.7)
    amidase, hydantoinase/carbamoylase family (TIGR01879; EC 3.5.-.-; HMM-score: 26.5)
    Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01246; EC 3.5.1.18; HMM-score: 24.2)
    Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01900; EC 3.5.1.18; HMM-score: 21.6)
    Metabolism Central intermediary metabolism Nitrogen metabolism allantoate amidohydrolase (TIGR03176; EC 3.5.3.9; HMM-score: 17.3)
  • TheSEED:  
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Lysine Biosynthesis DAP Pathway  N-acetyl-L,L-diaminopimelate deacetylase (EC 3.5.1.47)
  • PFAM:
    Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 114.2)
    and 2 more
    no clan defined M20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 38.3)
    Peptidase_MH (CL0035) Peptidase_M28; Peptidase family M28 (PF04389; HMM-score: 13.9)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004016
    • TAT(Tat/SPI): 0.000159
    • LIPO(Sec/SPII): 0.000508
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MIRVLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLSQLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHACGHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIYGTHLWSGYPTGTIHSRAGAIMASPDEFSVTFKGRGGHGAKPHETIDPIVIMAEFILSAQKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKLLQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSHYLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

  • NWMN_0511 no polycistronic organisation predicted

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]