PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0511 [new locus tag: NWMN_RS02970 ]
• pan locus tag^?: SAUPAN002321000
• symbol: NWMN_0511
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0511 [new locus tag: NWMN_RS02970 ]
• symbol: NWMN_0511
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 588713..589897
• length: 1185
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332574 NCBI
• RefSeq: YP_001331545 NCBI
• BioCyc:
• MicrobesOnline: 3706058 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  
  GTGATAAGGGTGTTAGATTGGTTCCAACTAGCAAATAATAAAGAAAATAAAACAATTCAA
  CTCAGACGTTATTTACACCAATATCCTGAGTTATCTTTTGAAGAATTTCAAACACATGAT
  TATATTGTTAACCAATTAAGCCAGTTATCTTGTGATATTGAAACTCCTATCGGCCGCAAT
  GGTATCAAAGCCACATTTAAAGGCTTGGGCACAGGACCTACCATTGCATTAAGAGCTGAC
  TTTGATGCGTTACCTGTCGAGGAATTAAACGATGTGCCTTATAAGTCTAAAAATCCAGGA
  TGTATGCATGCGTGTGGGCATGATGGTCATACAGCAATTTTACTTACTGTAGCAGAGATT
  TTAGATGAACATAAACATTTATTAGAAGGTAATGTTGTATTAATATTCCAGTACGGTGAA
  GAAATCATGCCAGGTGGTTCTCAAGAAATGATAGATGCTGGATGTCTGGAAAATGTAGAT
  AGAATTTACGGTACACATTTATGGAGTGGTTATCCTACTGGAACTATTCATTCACGTGCG
  GGGGCAATCATGGCCTCTCCAGATGAATTTAGCGTCACATTTAAAGGTCGTGGTGGTCAT
  GGTGCCAAACCTCATGAAACCATTGACCCAATAGTAATCATGGCAGAGTTCATTTTAAGT
  GCACAAAAAATAATTTCACGTACGATCGATCCAGTTAAACAAGCTGTACTTTCATTTGGA
  ATGATTCAAGCAGGCACCACAGATAGCGTTATTCCTGATCAAGCATTTTGTAAAGGTACA
  GTTAGAACTTTCGATTCAGATATACAGAATCATGTAATGGACAAAATGGATAAGCTTCTA
  CAAGGTTTAGCAATAGCAAATGATATTAACTATGATTTGAATTATATTAAAGGGTATTTG
  CCTGTCCATAATAACGAGAAAGCATATCAAGTTATTAAAGAAGCAACAAATGACTTACAT
  GTACGCTTTAATGAATCAGATTTAATGATGATTGGAGAAGATTTTTCACATTATTTGAAA
  GTACGACCAGGTGCATTCTTTCTGACTGGTTGTGGTAATGAAAGTAAAGGTATTACAGCG
  CCACACCATAATCCCAAATTTGATATTGATGAAAAATCATTAAAATACGCTGTAGCTGTA
  TTTTTAAAAATCATAGAATTAGAGCAAGTATTTAAAACAAATTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1185
  

⊟Protein

⊟General

• locus tag: NWMN_0511 [new locus tag: NWMN_RS02970 ]
• symbol: NWMN_0511
• description: hypothetical protein
• length: 394
• theoretical pI: 5.79675
• theoretical MW: 44025.9
• GRAVY: -0.223096

⊟Function

• TIGRFAM:
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides amidohydrolase (TIGR01891; HMM-score: 385.9)
  and 6 more
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 38)
  Metabolism Amino acid biosynthesis Glutamate family acetylornithine deacetylase (ArgE) (TIGR01892; EC 3.5.1.16; HMM-score: 30.7)
  amidase, hydantoinase/carbamoylase family (TIGR01879; EC 3.5.-.-; HMM-score: 26.5)
  Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01246; EC 3.5.1.18; HMM-score: 24.2)
  Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01900; EC 3.5.1.18; HMM-score: 21.6)
  Metabolism Central intermediary metabolism Nitrogen metabolism allantoate amidohydrolase (TIGR03176; EC 3.5.3.9; HMM-score: 17.3)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 114.2)
  and 2 more
  no clan defined M20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 38.3)
  Peptidase_MH (CL0035) Peptidase_M28; Peptidase family M28 (PF04389; HMM-score: 13.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004016
  • TAT(Tat/SPI): 0.000159
  • LIPO(Sec/SPII): 0.000508
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220723 NCBI
• RefSeq: YP_001331545 NCBI
• UniProt: A0A0H3K762 UniProt

⊟Protein sequence

• MIRVLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLSQLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHACGHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIYGTHLWSGYPTGTIHSRAGAIMASPDEFSVTFKGRGGHGAKPHETIDPIVIMAEFILSAQKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKLLQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSHYLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications