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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0544 [new locus tag: NWMN_RS03125 ]
  • pan locus tag?: SAUPAN002359000
  • symbol: ung
  • pan gene symbol?: ung
  • synonym:
  • product: uracil-DNA glycosylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0544 [new locus tag: NWMN_RS03125 ]
  • symbol: ung
  • product: uracil-DNA glycosylase
  • replicon: chromosome
  • strand: +
  • coordinates: 628655..629311
  • length: 657
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGAATGGTCGCAAATTTTTCATGACATAACAACGAAACATGACTTTAAAGCTATGCAT
    GATTTTTTAGAAAAAGAATATTCGACTGCAATCGTATACCCTGATAGGGAAAATATATAT
    CAAGCGTTTGATTTAACACCGTTTGAAAATATCAAAGTTGTTATATTAGGACAAGACCCG
    TATCATGGTCCAAACCAAGCACATGGATTAGCATTTTCAGTGCAACCTAACGCAAAATTC
    CCTCCATCTTTACGTAATATGTATAAAGAATTAGCAGATGATATTGGATGCGTTAGACAA
    ACACCGCATTTACAAGATTGGGCAAGAGAAGGCGTCTTGTTATTGAATACAGTTTTAACC
    GTAAGACAGGGTGAAGCAAATTCTCATCGTGATATTGGTTGGGAAACATTTACTGATGAA
    ATTATTAAAGCAGTGTCTGATTATAAAGAACATGTTGTCTTTATTTTGTGGGGGAAACCT
    GCACAGCAAAAAATAAAGCTTATCGATACATCTAAACATTGTATTATAAAATCAGTGCAT
    CCTAGTCCACTGTCTGCATATAGAGGATTCTTTGGATCAAAACCGTATTCCAAAGCGAAT
    GCCTATTTAGAGTCAGTAGGAAAATCACCAATTAATTGGTGTGAAAGTGAGGCGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    657

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0544 [new locus tag: NWMN_RS03125 ]
  • symbol: Ung
  • description: uracil-DNA glycosylase
  • length: 218
  • theoretical pI: 6.6759
  • theoretical MW: 24937.1
  • GRAVY: -0.425229

⊟Function[edit | edit source]

  • reaction:
    EC 3.2.2.27?  ExPASy
    Uracil-DNA glycosylase Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair uracil-DNA glycosylase (TIGR00628; EC 3.2.2.27; HMM-score: 263.2)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined UDG; Uracil DNA glycosylase superfamily (PF03167; HMM-score: 92.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002012
    • TAT(Tat/SPI): 0.000174
    • LIPO(Sec/SPII): 0.000334
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFDLTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTPHLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKPAQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKSPINWCESEA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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