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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0553 [new locus tag: NWMN_RS03170 ]
  • pan locus tag?: SAUPAN002371000
  • symbol: mvaK1
  • pan gene symbol?: mvaK1
  • synonym:
  • product: mevalonate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0553 [new locus tag: NWMN_RS03170 ]
  • symbol: mvaK1
  • product: mevalonate kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 637050..637970
  • length: 921
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGACAAGAAAAGGATATGGGGAATCGACAGGTAAGATTATTTTAATAGGAGAACATGCT
    GTTACATTTGGAGAGCCTGCTATTGCAGTACCGTTTAACGCAGGTAAAATCAAAGTTTTA
    ATAGAAGCCTTAGAGAGCGGGAACTATTCGTCTATTAAAAGCGATGTTTACGATGGTATG
    TTATATGATGCGCCTGACCATCTTAAGTCTTTGGTGAACCGTTTTGTAGAATTAAATAAT
    ATTACAGAGCCGCTAGCAGTAACGATCCAAACGAATTTACCACCATCACGTGGATTAGGA
    TCGAGTGCAGCTGTCGCGGTTGCTTTTGTTCGTGCAAGTTATGATTTTTTAGGGAAATCA
    TTAACGAAAGAAGAACTCATTGAAAAGGCTAATTGGGCAGAGCAAATTGCACATGGTAAA
    CCAAGTGGTATTGATACGCAAACGATTGTATCAGGCAAACCAGTTTGGTTCCAAAAAGGT
    CATGCTGAAACGTTGAAAACGTTAAGTTTAGACGGCTATATGGTTGTTATAGATACTGGT
    GTGAAAGGTTCAACAAGACAAGCAGTAGAAGATGTTCATAAACTTTGTGAGGACCCTCAG
    TACATGTCACATGTAAAACATATCGGTAAGTTAGTTTTACGTGCGAGTGATGTGATTGAA
    CATCATAACTTTGAAGCCTTAGCGGATATTTTTAATGAATGTCATGCGGATTTAAAGGCG
    TTGACAGTTAGTCATGATAAAATAGAACAATTAATGAAAATTGGTAAAGAAAATGGTGCG
    ATTGCTGGAAAACTTACTGGCGCTGGTCGTGGTGGAAGTATGTTATTGCTTGCCAAAGAT
    TTACCAACAGCGAAAAATATTGTAAAAGCTGTAGAAAAAGCTGGTGCAGCACATACTTGG
    ATTGAGAATTTAGGAGGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    921

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0553 [new locus tag: NWMN_RS03170 ]
  • symbol: MvaK1
  • description: mevalonate kinase
  • length: 306
  • theoretical pI: 6.96804
  • theoretical MW: 32918.5
  • GRAVY: -0.0977124

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Central intermediary metabolism Other mevalonate kinase (TIGR00549; EC 2.7.1.36; HMM-score: 290.8)
    and 6 more
    Metabolism Amino acid biosynthesis Aspartate family homoserine kinase (TIGR00191; EC 2.7.1.39; HMM-score: 46.8)
    Metabolism Energy metabolism Sugars galactokinase (TIGR00131; EC 2.7.1.6; HMM-score: 43.9)
    Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate kinase (TIGR01920; EC 2.7.1.71; HMM-score: 36.2)
    Metabolism Central intermediary metabolism Other phosphomevalonate kinase (TIGR01220; EC 2.7.4.2; HMM-score: 25.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase (TIGR00154; EC 2.7.1.148; HMM-score: 20.3)
    Metabolism Central intermediary metabolism Other diphosphomevalonate decarboxylase (TIGR01240; EC 4.1.1.33; HMM-score: 14.3)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    S5 (CL0329) GHMP_kinases_N; GHMP kinases N terminal domain (PF00288; HMM-score: 55.9)
    no clan defined GHMP_kinases_C; GHMP kinases C terminal (PF08544; HMM-score: 45.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.028291
    • TAT(Tat/SPI): 0.00151
    • LIPO(Sec/SPII): 0.002549
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEALESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSSAAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKGHAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDVIEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLLLAKDLPTAKNIVKAVEKAGAAHTWIENLGG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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