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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0585 [new locus tag: NWMN_RS03345 ]
  • pan locus tag?: SAUPAN002480000
  • symbol: NWMN_0585
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0585 [new locus tag: NWMN_RS03345 ]
  • symbol: NWMN_0585
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 666274..666780
  • length: 507
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAAAAAGCTACTAACGGCAAGTATAATTGCATGTTCTGTTGTAATGGGAGTAGGCTTA
    GTGAACACTAGTGCCGAAGCAGCAAGTGGCAACTCTATTGATACTGTTAAACAATTAATT
    AAGGGTGATCAGTCATTAGAAAATGTGAAAATTGGCGAATCTATTAAAGATGTTTTAACT
    AAGTACAAAAATCCGATGTATTCTTACAATGAAGATGGAACTGAACATTATTACGAATTC
    CATACTAAAAAAGGTATGTTATTAGTAACTACTGATGGTAAGAAAAACAATGGTAAAGTA
    ACTCATATTTCAATGATGTATAATGATGCTAATGGTCCAACATATCAAGCTGTTAAAAAT
    TATGTTGGCAAAGCAGTAACACATACGGAATATAGCAAAGTTGCTGGTAATTTTGGATAT
    ATTGAAAAAGGCAAAACGACTTATCAATTTGCCTCAGCACCAAAAGATAAAAACATAAAA
    TTATATCGTATTGATTTGGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    507

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_0585 [new locus tag: NWMN_RS03345 ]
  • symbol: NWMN_0585
  • description: hypothetical protein
  • length: 168
  • theoretical pI: 9.59726
  • theoretical MW: 18594.1
  • GRAVY: -0.497024

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    NTF2 (CL0051) DUF5104; Domain of unknown function (DUF5104) (PF17117; HMM-score: 11)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helix: 1
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.33
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: TSAEAASG
  • SignalP: Signal peptide SP(Sec/SPI) length 27 aa
    • SP(Sec/SPI): 0.997919
    • TAT(Tat/SPI): 0.000501
    • LIPO(Sec/SPII): 0.001324
    • Cleavage Site: CS pos: 27-28. AEA-AS. Pr: 0.9493
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKKLLTASIIACSVVMGVGLVNTSAEAASGNSIDTVKQLIKGDQSLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTHISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIKLYRIDLEK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]