PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0581 [new locus tag: NWMN_RS03325 ]
• pan locus tag^?: SAUPAN002476000
• symbol: NWMN_0581
• pan gene symbol^?: —
• synonym:
• product: iron compound ABC transporter iron compound-binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0581 [new locus tag: NWMN_RS03325 ]
• symbol: NWMN_0581
• product: iron compound ABC transporter iron compound-binding protein
• replicon: chromosome
• strand: +
• coordinates: 662595..663482
• length: 888
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330319 NCBI
• RefSeq: YP_001331615 NCBI
• BioCyc:
• MicrobesOnline: 3706128 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  GTGAAGAAATCGTTAATTGCTTTTATTTTGATTTTTATGCTTGTCCTGAGTGGCTGTGGT
  ATGAAAGATAATGATAAACAAGGTAGCAATGATAATGGCTCGTCTAAATCGCCGTACCAT
  AGAATTGTTTCGTTAATGCCTAGTAATACTGAAATTTTATATGAATTAGGATTAGGTAAA
  TACATAGTTGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAGAAA
  CAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATT
  CTTGCGCATGAGTCGCAAAAGGCAACTGCTAATAAAGTATTGTCATCATTAGAGAAACAA
  GGCATCAAAGTAGTGTATGTTAAAGATGCACAATCAATTGATGAAACTTACAACACATTT
  AAGCAAATTGGGAAATTAACGCATCATGATAAGCAGGCTGAACAACTTGTTGAGGAAACT
  AAAGATAATATCGATAAAGTCATAGATTCAATTCCTGCTCATCATAAAAAATCAAAAGTA
  TTTATTGAGGTTTCATCAAAGCCTGAAATATATACAGCAGGGAAGCATACATTTTTTAAT
  GATATGTTAGAAAAATTAGAAGCCCAAAATGTGTATAGTGACATTAATGGTTGGAACCCT
  GTAACGAAGGAAAGTATTATTAAAAAGAACCCAGATATATTAATTTCGACGGAAGCTAAG
  ACAAGATCAGATTATATGGATATCATCAAAAAAAGAGGTGGATTCAATAAAATTAATGCT
  GTCAAGAATACACGTATTGAAGTTGTAAATGGTGATGAAGTATCAAGACCAGGTCCACGT
  ATTGATGAAGGATTAAAAGAATTAAGAGATGCAATTTATAGAAAATAA
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⊟Protein

⊟General

• locus tag: NWMN_0581 [new locus tag: NWMN_RS03325 ]
• symbol: NWMN_0581
• description: iron compound ABC transporter iron compound-binding protein
• length: 295
• theoretical pI: 9.53333
• theoretical MW: 33254.9
• GRAVY: -0.574237

⊟Function

• TIGRFAM:
  putative ABC transporter PGF-CTERM-modified substrate-binding protein (TIGR04281; HMM-score: 125)
  and 2 more
  heme ABC transporter, heme-binding protein isdE (TIGR03659; HMM-score: 75.4)
  proposed F420-0 ABC transporter, periplasmic F420-0 binding protein (TIGR03868; HMM-score: 54)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Chelatase (CL0043) Peripla_BP_2; Periplasmic binding protein (PF01497; HMM-score: 117.3)
  and 7 more
  HTH (CL0123) F-112; F-112 protein (PF09645; HMM-score: 17.6)
  no clan defined MMR1; Mitochondrial Myo2 receptor-related protein (PF08505; HMM-score: 14.8)
  ETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 14)
  LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 13.7)
  Chelatase (CL0043) Oxidored_nitro; Nitrogenase component 1 type Oxidoreductase (PF00148; HMM-score: 13.5)
  Periplas_BP (CL0144) Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 13.2)
  SIS (CL0067) DUF2529; Domain of unknown function (DUF2529) (PF10740; HMM-score: 12.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Lipid anchored
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: 0.33
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: LVLSGCG
• SignalP: Signal peptide LIPO(Sec/SPII) length 18 aa
  • SP(Sec/SPI): 0.000399
  • TAT(Tat/SPI): 0.00006
  • LIPO(Sec/SPII): 0.999338
  • Cleavage Site: CS pos: 18-19. LSG-CG. Pr: 0.9999
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220793 NCBI
• RefSeq: YP_001331615 NCBI
• UniProt: A0A0H3K6J6 UniProt

⊟Protein sequence

• MKKSLIAFILIFMLVLSGCGMKDNDKQGSNDNGSSKSPYHRIVSLMPSNTEILYELGLGKYIVGVSTVDDYPKDVKKGKKQFDALNLNKEELLKAKPDLILAHESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEETKDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGWNPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSRPGPRIDEGLKELRDAIYRK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0581 > NWMN_0582 > NWMN_0583 > NWMN_0584 > NWMN_0585

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications