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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0566 [new locus tag: SA_RS03275 ]
  • pan locus tag?: SAUPAN002476000
  • symbol: SA0566
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA0566 [new locus tag: SA_RS03275 ]
  • symbol: SA0566
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 660074..660961
  • length: 888
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    GTGAAGAAATCGTTAATTGCTTTTATTTTGATTTTTATGCTTGTCCTGAGTGGCTGTGGT
    ATGAAAGATAATGATAAACAAGGTAGCGATGATAATGGCTCGTCTAAATCGCCGTACCAT
    AGAATTGTTTCGTTAATGCCTAGTAATACTGAAATTTTATATGAATTAGGATTAGGTAAA
    TACATAGTTGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAGAGGGTAAGAAA
    CAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATT
    CTTGCGCATGAGTCGCAAAAGGCAACTGCTAATAAAGTATTGTCATCATTAGAGAAACAA
    GGCATCAAAGTAGTGTATGTTAAAGATGCACAATCAATTGATGAAACATACAACACATTT
    AAGCAAATTGGGAAATTAACGCATCATGATAAGCAGGCTGAACAACTTGTTGAGGAAACT
    AAAGATAATATCGATAAAGTCATAGATTCAATTCCTGCTCATCATAAAAAATCAAAAGTA
    TTTATTGAGGTTTCATCAAAGCCTGAAATATATACAGCAGGGAAGCATACATTTTTTAAT
    GATATGTTAGAAAAATTAGAAGCCCAAAATGTTTATAGTGACATTAATGGTTGGAACCCT
    GTAACGAAGGAAAGTATTATTAAAAAGAACCCAGATATATTAATTTCGACGGAAGCTAAG
    ACAAGATCAGATTATATGGATATCATCAAAAAAAGGGGTGGATTCAATAAAATTAATGCT
    GTCAAGAATACACGTATTGAAGTTGTAAATGGTGATGAAGTATCAAGACCAGGTCCACGT
    ATTGATGAAGGATTAAAAGAATTAAGAGATGCAATTTATAGAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    888

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA0566 [new locus tag: SA_RS03275 ]
  • symbol: SA0566
  • description: hypothetical protein
  • length: 295
  • theoretical pI: 9.04982
  • theoretical MW: 33256.8
  • GRAVY: -0.572881

Function[edit | edit source]

  • TIGRFAM:
    putative ABC transporter PGF-CTERM-modified substrate-binding protein (TIGR04281; HMM-score: 125.4)
    and 2 more
    heme ABC transporter, heme-binding protein isdE (TIGR03659; HMM-score: 75)
    proposed F420-0 ABC transporter, periplasmic F420-0 binding protein (TIGR03868; HMM-score: 54.1)
  • TheSEED  :
    • Vitamin B12 ABC transporter, B12-binding component BtuF
  • PFAM:
    Chelatase (CL0043) Peripla_BP_2; Periplasmic binding protein (PF01497; HMM-score: 117.4)
    and 7 more
    HTH (CL0123) F-112; F-112 protein (PF09645; HMM-score: 17.5)
    no clan defined MMR1; Mitochondrial Myo2 receptor-related protein (PF08505; HMM-score: 14.9)
    LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 13.7)
    Chelatase (CL0043) Oxidored_nitro; Nitrogenase component 1 type Oxidoreductase (PF00148; HMM-score: 13.6)
    no clan defined ETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 13.5)
    SIS (CL0067) DUF2529; Domain of unknown function (DUF2529) (PF10740; HMM-score: 13.4)
    Periplas_BP (CL0144) Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 13.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: 0.33
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: LVLSGCG
  • SignalP: Signal peptide LIPO(Sec/SPII) length 18 aa
    • SP(Sec/SPI): 0.000402
    • TAT(Tat/SPI): 0.00006
    • LIPO(Sec/SPII): 0.999333
    • Cleavage Site: CS pos: 18-19. LSG-CG. Pr: 0.9999
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKKSLIAFILIFMLVLSGCGMKDNDKQGSDDNGSSKSPYHRIVSLMPSNTEILYELGLGKYIVGVSTVDDYPKDVKEGKKQFDALNLNKEELLKAKPDLILAHESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEETKDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGWNPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSRPGPRIDEGLKELRDAIYRK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]