From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS03275 [old locus tag: SA0566 ]
  • pan locus tag?: SAUPAN002476000
  • symbol: SA_RS03275
  • pan gene symbol?: —
  • synonym:
  • product: ABC transporter substrate-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS03275 [old locus tag: SA0566 ]
  • symbol: SA_RS03275
  • product: ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 660134..660961
  • length: 828
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (660134..660961) NCBI
  • BioCyc: SA_RS03275 BioCyc
  • MicrobesOnline: see SA0566

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAAGATAATGATAAACAAGGTAGCGATGATAATGGCTCGTCTAAATCGCCGTACCAT
    AGAATTGTTTCGTTAATGCCTAGTAATACTGAAATTTTATATGAATTAGGATTAGGTAAA
    TACATAGTTGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAGAGGGTAAGAAA
    CAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATT
    CTTGCGCATGAGTCGCAAAAGGCAACTGCTAATAAAGTATTGTCATCATTAGAGAAACAA
    GGCATCAAAGTAGTGTATGTTAAAGATGCACAATCAATTGATGAAACATACAACACATTT
    AAGCAAATTGGGAAATTAACGCATCATGATAAGCAGGCTGAACAACTTGTTGAGGAAACT
    AAAGATAATATCGATAAAGTCATAGATTCAATTCCTGCTCATCATAAAAAATCAAAAGTA
    TTTATTGAGGTTTCATCAAAGCCTGAAATATATACAGCAGGGAAGCATACATTTTTTAAT
    GATATGTTAGAAAAATTAGAAGCCCAAAATGTTTATAGTGACATTAATGGTTGGAACCCT
    GTAACGAAGGAAAGTATTATTAAAAAGAACCCAGATATATTAATTTCGACGGAAGCTAAG
    ACAAGATCAGATTATATGGATATCATCAAAAAAAGGGGTGGATTCAATAAAATTAATGCT
    GTCAAGAATACACGTATTGAAGTTGTAAATGGTGATGAAGTATCAAGACCAGGTCCACGT
    ATTGATGAAGGATTAAAAGAATTAAGAGATGCAATTTATAGAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    828

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS03275 [old locus tag: SA0566 ]
  • symbol: SA_RS03275
  • description: ABC transporter substrate-binding protein
  • length: 275
  • theoretical pI: 8.60866
  • theoretical MW: 31090
  • GRAVY: -0.746909

⊟Function[edit | edit source]

  • TIGRFAM:
    putative ABC transporter PGF-CTERM-modified substrate-binding protein (TIGR04281; HMM-score: 125.7)
    and 2 more
    heme ABC transporter, heme-binding protein isdE (TIGR03659; HMM-score: 61.6)
    proposed F420-0 ABC transporter, periplasmic F420-0 binding protein (TIGR03868; HMM-score: 54.5)
  • TheSEED: see SA0566
  • PFAM:
    Chelatase (CL0043) Peripla_BP_2; Periplasmic binding protein (PF01497; HMM-score: 117.8)
    and 5 more
    HTH (CL0123) F-112; F-112 protein (PF09645; HMM-score: 17.7)
    no clan defined MMR1; Mitochondrial Myo2 receptor-related protein (PF08505; HMM-score: 15.1)
    Chelatase (CL0043) Oxidored_nitro; Nitrogenase component 1 type Oxidoreductase (PF00148; HMM-score: 14)
    SIS (CL0067) DUF2529; Domain of unknown function (DUF2529) (PF10740; HMM-score: 13.5)
    Periplas_BP (CL0144) Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 13.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006446
    • TAT(Tat/SPI): 0.000357
    • LIPO(Sec/SPII): 0.000845
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKDNDKQGSDDNGSSKSPYHRIVSLMPSNTEILYELGLGKYIVGVSTVDDYPKDVKEGKKQFDALNLNKEELLKAKPDLILAHESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEETKDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGWNPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSRPGPRIDEGLKELRDAIYRK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SA_RS03275&oldid=92683"