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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0597 [new locus tag: NWMN_RS03405 ]
  • pan locus tag?: SAUPAN002498000
  • symbol: mnhE
  • pan gene symbol?: mnhE2
  • synonym:
  • product: putative monovalent cation/H+ antiporter subunit E

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0597 [new locus tag: NWMN_RS03405 ]
  • symbol: mnhE
  • product: putative monovalent cation/H+ antiporter subunit E
  • replicon: chromosome
  • strand: +
  • coordinates: 677136..677618
  • length: 483
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAATCAAATAGTTTTAAATATTATCATTGCATTCTTATGGGTATTATTTCAAGATGAA
    GATCATTTTAAATTCTCGACTTTCTTTTCTGGATATCTAATTGGTTTAATTGTCATTTAT
    ATATTACACAGGTTTTTCAGCGATGATTTTTATGTTAGAAAAATATGGGTAGCTATTAAA
    TTTTTAGGTGTTTATTTATATCAATTAATAACATCTAGCATTAGCACGATTAATTATATT
    CTTTTTAAAACAAAAGATATGAACCCTGGATTACTTTCATATGAAACAAGACTAACAAGT
    GATTGGTCAATAACATTTTTAACAATTTTAATTATTATAACTCCAGGGTCTACAGTAATA
    CGAATTTCTCAAGACTCTAAAAAGTTTTTTATTCATAGTATCGACGTGTCAGAAAAAGAA
    AAAGATAGTTTGTTAAGAAGTATTAAGCATTATGAAGACTTAATATTGGAGGTGTCGCGA
    TGA
    60
    120
    180
    240
    300
    360
    420
    480
    483

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0597 [new locus tag: NWMN_RS03405 ]
  • symbol: MnhE
  • description: putative monovalent cation/H+ antiporter subunit E
  • length: 160
  • theoretical pI: 7.80816
  • theoretical MW: 18850.9
  • GRAVY: 0.42125

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds multicomponent Na+:H+ antiporter (TIGR00942; HMM-score: 79.2)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined MNHE; Na+/H+ ion antiporter subunit (PF01899; HMM-score: 110.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002095
    • TAT(Tat/SPI): 0.000061
    • LIPO(Sec/SPII): 0.014667
  • predicted transmembrane helices (TMHMM): 3

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHRFFSDDFYVRKIWVAIKFLGVYLYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDWSITFLTILIIITPGSTVIRISQDSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1] [2]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  2. ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J Bacteriol: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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