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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0615 [new locus tag: NWMN_RS03500 ]
  • pan locus tag?: SAUPAN002524000
  • symbol: NWMN_0615
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0615 [new locus tag: NWMN_RS03500 ]
  • symbol: NWMN_0615
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 697374..698207
  • length: 834
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    GTGAATAAAACGGTTAAAGATTTAATACTAGTTGTCTTAGGTTCATTTATCTTTGCTGCA
    GGTGTAAATGCATTTATTATTTCTGGTAACTTAGGTGAAGGCGGGGTTACAGGTTTAGCA
    ATTATTTTATATTATGCGTTTCATATTTCACCAGCCATCACTAACTTCTTGGTCAACGCA
    GTATTGATTGCCATAGGTTATAAATTTTTGAGTAAGAGAAGTATGTACTTAACTATTCTT
    GTAACAATTCTTATTTCAATATTTTTGAGTTTAACAGAATCATGGCAAGTAGAAACTGGA
    AACAGCATTGTGAATGCCATTTTTGGTGGTGTAAGCGTTGGACTAGGAATCGGAGTAATT
    ATCCTTGCAGGCGGTACAACAGCAGGTACAACAATTTTGGCGAGAATTGCAACGAAATAC
    CTCGATGTAAGCACGCCATATGCTTTGCTTTTCTTCGATATGATCGTTGTTGCAATTTCA
    CTTACAGTTATTCCACTTGATAAAGTATTAGTAACAGTAATATCACTTTATATAGGAACA
    AAAGTGATGGAATATGTCATAGAAGGTTTAAACACTAAAAAAGCTATGACGATTATTTCA
    ACTAATCCCGACAAACTTGCCAAAGCAATAGACGAGCAAATTGGAAGAGGTTTAACCATT
    TTAAACGGACATGGCTATTATACGCGTGAAGAAAAAGATGTCTTATACGTTGTTATTTCT
    AAAACACAAGTTTCAAAAGCAAAGCGATTAATTAAACAAATCGATAAAGATGCATTCCTC
    GTAATTCATGATGTAAGAGATGTCTATGGTAATGGCTTTCTTGCAGATGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    834

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0615 [new locus tag: NWMN_RS03500 ]
  • symbol: NWMN_0615
  • description: hypothetical protein
  • length: 277
  • theoretical pI: 9.04665
  • theoretical MW: 29917.1
  • GRAVY: 0.708664

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined YitT_membrane; Uncharacterised 5xTM membrane BCR, YitT family COG1284 (PF02588; HMM-score: 198.6)
    and 3 more
    GlnB-like (CL0089) DUF2179; Uncharacterized protein conserved in bacteria (DUF2179) (PF10035; HMM-score: 77.3)
    no clan defined ODV-E18; Occlusion-derived virus envelope protein ODV-E18 (PF10717; HMM-score: 6.7)
    DUF2070; Predicted membrane protein (DUF2070) (PF09843; HMM-score: 5.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 5
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.13398
    • TAT(Tat/SPI): 0.001159
    • LIPO(Sec/SPII): 0.095849
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILYYAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNSIVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAISLTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGLTILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLADE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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