PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0660 [new locus tag: NWMN_RS03740 ]
• pan locus tag^?: SAUPAN002575000
• symbol: NWMN_0660
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0660 [new locus tag: NWMN_RS03740 ]
• symbol: NWMN_0660
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 741608..742981
• length: 1374
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330375 NCBI
• RefSeq: YP_001331694 NCBI
• BioCyc:
• MicrobesOnline: 3706207 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  
  ATGGCAATTGCTGTGTTATTAAATCGAATGTTTCGAATGGAACACAATCCATTATTTGAA
  TATATTTATCAACAAAAAGAAGACATTGATGCATGTTATTTTATCATTCCGGAAGAGGAC
  ATGTCTTCAGCTTCTGATTTGAAAGCACAGTTTTATCGCGGTACTTTGCAGCGCTTTTAC
  CAATCGTTGCACGCAGAAAAGCTTACACCTTATGTTATGTCTTATGACGATATCATTTCA
  TTTTGTAAAGAAAACAATATCTCTGAAGTAGTGACTGCGGGTGATATTATGAGTTATCAT
  CTTGAAGAATATGATATTTTACATCAACGTTCTTTATTCAATGAAGCACGCATTGCCGTT
  ACTTTGATACGTGGGAATCATTACTTTAAAGCGAGTAAAACAATGAATCAACAAGGGGAG
  CCATACAATGTTTTTACTAGTTTCTATAAAAAATGGCGACCTTACTTGAGGCATAGAGAC
  GTATATCACTATGATTTAAAATCATTCGAAGACTTTGTCATTGCATCACCTGATGATTTA
  GTGTTTGATGACATAGCATTTGGATCCTCACAAATAATTGAACAGAATAAATGGCAACAT
  TTTTTAGATCAAGATATACAGAATTACGAAAGCGGAAGAGACTATTTACCTGAAGTATTA
  ACAAGTCAGCTAAGTGTTGCTTTAGCATATGGATTATTAGATATTATTGAAATTTTTAAT
  GATTTATTGGCGCGTTATGATGAAGATGAGGCAAACTATGAAGCATTTATACGTGAACTC
  ATTTTTAGAGAATTTTATTATGTGTTAATGACACAGTATCCTGAAACCTCATACCAAGCT
  TTCAAACCTAAATATCGACAGATAAAATGGTCGCAAAATGAAGCGGATTTTAATGCATGG
  TGCGAAGGGCAAACAGGATTTCCAATCATTGATGCAGCAATAATGGAATTGACACAAACT
  GGTTTTATGCATAATCGAATGAGAATGGTTGTGTCGCAATTTTTAACCAAAGATTTATTT
  ATAGATTGGACATGGGGAGAAAAATTCTTTAGAAAGCACCTTATTGACTATGATGCAGCA
  TCAAATATTCATGGATGGCAATGGTCTGCTTCTACAGGTACGGATGCAGTGCCGTATTTT
  AGAATGTTTAATCCAATAAGACAGAGTGAACGCTTTGATGCTAAAGCTTTGTATATCAAA
  ACATATCTTCCGATTTTTAATCAAATTGATGCAAAATATTTGCATGATACACAACGCAAT
  GAGTCCAACCTTTTTGAACAGGGGATTGAATTAGGTAGTCATTATCCAAGACAAATGGTA
  GATCATCAAGAAAAACGTACACAAGTTTTAGCTACATTTAAAGCGCTAGACTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1374
  

⊟Protein

⊟General

• locus tag: NWMN_0660 [new locus tag: NWMN_RS03740 ]
• symbol: NWMN_0660
• description: hypothetical protein
• length: 457
• theoretical pI: 4.9108
• theoretical MW: 54369.9
• GRAVY: -0.449015

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair deoxyribodipyrimidine photo-lyase, 8-HDF type (TIGR03556; EC 4.1.99.3; HMM-score: 249.3)
  and 4 more
  cryptochrome, plant family (TIGR02766; HMM-score: 149.8)
  Cellular processes Cellular processes Adaptations to atypical conditions cryptochrome, DASH family (TIGR02765; HMM-score: 149.2)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair deoxyribodipyrimidine photolyase (TIGR00591; EC 4.1.99.3; HMM-score: 17.7)
  Unknown function Enzymes of unknown specificity putative phosphoesterase (TIGR00024; EC 3.1.-.-; HMM-score: 13.9)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined FAD_binding_7; FAD binding domain of DNA photolyase (PF03441; HMM-score: 250.2)
  and 2 more
  HUP (CL0039) DNA_photolyase; DNA photolyase (PF00875; HMM-score: 44.2)
  Calcineurin (CL0163) Metallophos; Calcineurin-like phosphoesterase (PF00149; HMM-score: 14.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001389
  • TAT(Tat/SPI): 0.000095
  • LIPO(Sec/SPII): 0.000339
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220872 NCBI
• RefSeq: YP_001331694 NCBI
• UniProt: A0A0H3K7F8 UniProt

⊟Protein sequence

• MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSASDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVTAGDIMSYHLEEYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYNVFTSFYKKWRPYLRHRDVYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPEVLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPETSYQAFKPKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQFLTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERFDAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVLATFKALD

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications