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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0754 [new locus tag: NWMN_RS04270 ]
  • pan locus tag?: SAUPAN002777000
  • symbol: NWMN_0754
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0754 [new locus tag: NWMN_RS04270 ]
  • symbol: NWMN_0754
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 848304..848825
  • length: 522
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGATTAACATTATTTCAGCTATAGGATCTATTGGAACATTTATTATGGCTTTATTTTAT
    TTTGTATCAGTTTCAGTTCAACTTTATCAAATGAAAATTAGCTTTCTGCCAGCTTTAGGT
    TTTAACCAAATTTTATTAGAAAGGGAGGAGGATCAACTTAATATAATGAATTCGGCAACA
    GAAGAGCATCATCATAAAGATTATATTAAACTATATAATTTAGGTGGCGGTGCTGCTAAA
    AAAATTGCAATAGAGGTTTTATTGGGGAAGGATAAAGTCATTCAGAAAAAATACGTGCAT
    ATTTTACCTAGTAAAGAAGGGTACATGTTACCAATTAATAAAAATGTGTACGAAGAATTA
    GAAAGAACGATTGAGAACAATGGTCATGAAGCTGATTTGAATGTACGTATGACTTATTAT
    CATAATGTAAGTCGCAAACAACAGGAAGTTATATTAAAAGGTCAAATCGACCGTTTTAAT
    ACTTATAATAATAAAGAAATTTATGATTTGCAGTTTATCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    522

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0754 [new locus tag: NWMN_RS04270 ]
  • symbol: NWMN_0754
  • description: hypothetical protein
  • length: 173
  • theoretical pI: 7.17939
  • theoretical MW: 20088.1
  • GRAVY: -0.218497

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    E-set (CL0159) CARDB; CARDB (PF07705; HMM-score: 17.4)
    and 1 more
    RAMPS-Cas5-like (CL0362) Cas_Cas6; CRISPR associated protein Cas6 (PF01881; HMM-score: 13.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 7
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.021373
    • TAT(Tat/SPI): 0.000409
    • LIPO(Sec/SPII): 0.004302
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MINIISAIGSIGTFIMALFYFVSVSVQLYQMKISFLPALGFNQILLEREEDQLNIMNSATEEHHHKDYIKLYNLGGGAAKKIAIEVLLGKDKVIQKKYVHILPSKEGYMLPINKNVYEELERTIENNGHEADLNVRMTYYHNVSRKQQEVILKGQIDRFNTYNNKEIYDLQFI

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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