PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0930 [new locus tag: NWMN_RS05205 ]
• pan locus tag^?: SAUPAN003270000
• symbol: qoxA
• pan gene symbol^?: qoxA
• synonym:
• product: quinol oxidase polypeptide II QoxA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0930 [new locus tag: NWMN_RS05205 ]
• symbol: qoxA
• product: quinol oxidase polypeptide II QoxA
• replicon: chromosome
• strand: -
• coordinates: 1032239..1033339
• length: 1101
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332327 NCBI
• RefSeq: YP_001331964 NCBI
• BioCyc:
• MicrobesOnline: 3706481 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  121
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  481
  541
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  661
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  1021
  1081
  
  GTGTCAAAATTTAAGTCTTTGCTTCTATTATTTGGCACACTAATTTTACTTAGTGGTTGT
  TCAAATATAGAAATTTTCAACGCAAAAGGGCCAGTAGCAAGTAGTCAGAAGTTCTTGATT
  CTTTATTCAATCGTCTTCATGCTTGTTATTTGTTTCGTTGTACTTGGCATGTTCGCCATT
  TTTATTTACAAGTATAGTTATAATAAGAATGCCGAATCTGGTAAGATGCACCATAATGCC
  ATCATTGAAACAATATGGTTTGTAATACCTATCATAATCGTTGCTGCTTTAGCTATTCCT
  ACAGTTAAAACTTTATACGATTACGAAAAACCACCGAAGAGTGAGAAAGATCCAATGGTT
  GTATATGCAGTAAGTGCAGGATACAAATGGTTCTTTGCTTATCCAGATGAACATATAGAG
  ACTGTTAATACATTAACAATCCCTAAAGATCGTCCTGTTGTATTTAAGCTTCAAGCTATG
  GATACAATGACAAGTTTCTGGATTCCACAATTAGGTGGTCAAAAATATGCCATGACTGGC
  ATGACAATGAATTGGACGTTAGAAGCATCTCAAACTGGTACGTTCAGAGGTCGTAACTCT
  AACTTCAATGGTGAAGGATTCTCACGTCAAACATTTAAAGTAAATGCCGTTAGTCAAAAA
  GACTACGACAAATGGGTGAAAGAAGTTAAAGGTAAGAAAACGTTAGATCAAGATACATTT
  GATAAACAATTATTACCAAGCACACCTAATAAAGCTTTAGAATTTAATGGTACACATATG
  GCGTTTGTTGATCCAGCAGCCGATCCAGAATACATCTTCTATGCATATAAACGTTTCAAT
  TTCGAATTGAAAGATCCAAACTTCACTAGTGAAGAAAATATGTTTAAAGATGTATCAGAC
  AAACCATTAATACCTGCTCGTAAAGCTCAAATTACAAATGCGAACTATAAACGACATGGT
  ATGAAGTTGATGATTCTTGGAAATGACGAACCATATAACAATGAGTTCAAGAAAGATGAA
  TCGAAAAATGCGAAAGAAATGAAGAAAATTTCAAAAGATGCGCAAGATCAAGACAATGAT
  GATCATGGAGGTGGACATTAA
  60
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  1101
  

⊟Protein

⊟General

• locus tag: NWMN_0930 [new locus tag: NWMN_RS05205 ]
• symbol: QoxA
• description: quinol oxidase polypeptide II QoxA
• length: 366
• theoretical pI: 9.00291
• theoretical MW: 41776.8
• GRAVY: -0.337158

⊟Function

• reaction:
  EC 1.10.3.-?  ExPASy
  2 a quinol + O₂ = 2 a quinone + 2 H₂O?
• TIGRFAM:
  Metabolism Energy metabolism Electron transport cytochrome aa3 quinol oxidase, subunit II (TIGR01432; EC 1.10.3.-; HMM-score: 430.2)
  and 2 more
  Metabolism Energy metabolism Electron transport ubiquinol oxidase, subunit II (TIGR01433; EC 1.10.3.-; HMM-score: 181.3)
  Metabolism Energy metabolism Electron transport cytochrome c oxidase, subunit II (TIGR02866; EC 1.9.3.1; HMM-score: 135.6)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined COX2_TM; Cytochrome C oxidase subunit II, transmembrane domain (PF02790; HMM-score: 44.7)
  and 9 more
  CU_oxidase (CL0026) COX2; Cytochrome C oxidase subunit II, periplasmic domain (PF00116; HMM-score: 23.8)
  LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 17.8)
  CU_oxidase (CL0026) Cupredoxin_1; Cupredoxin-like domain (PF13473; HMM-score: 13.4)
  no clan defined Bac_export_2; FlhB HrpN YscU SpaS Family (PF01312; HMM-score: 12.3)
  HRG; Haem-transporter, endosomal/lysosomal, haem-responsive gene (PF16954; HMM-score: 11.3)
  Bunya_G2; Bunyavirus glycoprotein G2 (PF03563; HMM-score: 9.9)
  DHHC; DHHC palmitoyltransferase (PF01529; HMM-score: 8.7)
  BPD_transp_1 (CL0404) FtsX; FtsX-like permease family (PF02687; HMM-score: 7.8)
  no clan defined RELT; Tumour necrosis factor receptor superfamily member 19 (PF12606; HMM-score: 7.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 3
• LocateP: Multi-transmembrane(Lipid modified N-termini)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: 0
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: ILLSGCS
• SignalP: Signal peptide LIPO(Sec/SPII) length 19 aa
  • SP(Sec/SPI): 0.000508
  • TAT(Tat/SPI): 0.000057
  • LIPO(Sec/SPII): 0.998769
  • Cleavage Site: CS pos: 19-20. LSG-CS. Pr: 0.9995
• predicted transmembrane helices (TMHMM): 3

⊟Accession numbers

• GI: 151221142 NCBI
• RefSeq: YP_001331964 NCBI
• UniProt: A0A0H3K811 UniProt

⊟Protein sequence

• MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSIVFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTVKTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAMDTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVSQKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAYKRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNNEFKKDESKNAKEMKKISKDAQDQDNDDHGGGH

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: qoxD < qoxC < qoxB < qoxA

⊟Regulation

• data available for NCTC8325

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications