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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1230 [new locus tag: NWMN_RS06950 ]
  • pan locus tag?: SAUPAN003683000
  • symbol: NWMN_1230
  • pan gene symbol?: cls1
  • synonym:
  • product: phospholipase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1230 [new locus tag: NWMN_RS06950 ]
  • symbol: NWMN_1230
  • product: phospholipase
  • replicon: chromosome
  • strand: +
  • coordinates: 1353887..1355395
  • length: 1509
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    GTGCCTTTCTTTATAGGAGAAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACG
    TTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATT
    ATCTTTTTAGAAAGAAATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTT
    TTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCA
    CGCAAATTGAATAAAAACAATGGTAACGTGTTAACGGATTTCGATGGACTTTTAAAACAA
    CAAATAGAAAGCTTTGATAAAGGTAATTATGGTACTGATAACAAACAAGTTCAAAAACAT
    CATGATTTAGTACGTATGCTTTTGATGGATCAAGATGGTTTTTTAACTGAAAATAATAAA
    GTTGATCATTTCATTGATGGAAATGATTTATATGATCAAGTTTTAAAAGATATTAAAAAT
    GCAAAAGAATATATCCATTTAGAGTACTATACTTTCGCTTTAGATGGTTTAGGTAAAAGA
    ATTTTACATGCTTTAGAAGAAAAATTGAAACAAGGTCTAGAAGTAAAAATATTATATGAT
    GATGTTGGATCTAAAAATGTTAAGATGGCAAATTTTGATCATTTTAAATCGTTAGGTGGA
    GAAGTTGAAGCATTTTTTGCTTCAAAATTACCGTTATTGAATTTCAGAATGAATAATAGA
    AATCATAGAAAAATCATCGTAATCGATGGTCAACTAGGTTATGTCGGAGGATTTAACATT
    GGTGATGAATATCTAGGATTAGGAAAATTAGGATATTGGAGAGATACGCATTTACGTATA
    CAAGGGGATGCGGTTGATGCACTGCAGTTGCGATTTATTTTAGACTGGAATTCGCAAGCG
    CACCGTCCACAATTTGAATATGATGTTAAGTATTTCCCTAAAAAGAACGGACCATTGGGC
    AATTCACCAATTCAAATAGCTGCAAGTGGCCCGGCTAGTGACTGGCATCAAATTGAATAC
    GGTTATACAAAAATGATTATGAGTGCAAAGAAATCTGTATATTTACAATCACCATATTTC
    ATTCCGGATAATTCATATATAAATGCCATTAAAATTGCTGCTAAATCAGGTGTAGATGTA
    CATTTAATGATTCCATGTAAGCCAGATCATCCATTAGTATATTGGGCGACATTTTCAAAT
    GCCTCTGACTTATTATCAAGTGGTGTTAAAATTTATACGTATGAAAATGGATTTATACAT
    TCTAAAATGTGCTTAATTGATGATGAAATCGTATCAGTGGGCACAGCAAATATGGACTTT
    AGAAGTTTTGAATTAAATTTTGAAGTAAATGCCTTTGTATATGATGAAAATCTTGCTAAA
    GATTTAAGGGTGGCTTATGAACATGATATTACAAAATCAAAACAACTAACCAAAGAATCA
    TATGCCAATAGACCGCTGTCTGTTAAATTCAAAGAATCGTTAGCAAAATTAGTTTCGCCA
    ATTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1509

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1230 [new locus tag: NWMN_RS06950 ]
  • symbol: NWMN_1230
  • description: phospholipase
  • length: 502
  • theoretical pI: 7.81952
  • theoretical MW: 57416.6
  • GRAVY: -0.136653

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.8.-?  ExPASy
    2 Phosphatidylglycerol = diphosphatidylglycerol + glycerol?
  • TIGRFAM:
    cardiolipin synthase (TIGR04265; EC 2.7.8.-; HMM-score: 822.2)
  • TheSEED  :
    • Cardiolipin synthetase (EC 2.7.8.-)
    Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Cardiolipin synthetase (EC 2.7.8.-)
  • PFAM:
    PLD (CL0479) PLDc_2; PLD-like domain (PF13091; HMM-score: 154.2)
    and 10 more
    PLDc; Phospholipase D Active site motif (PF00614; HMM-score: 58.5)
    no clan defined PLDc_N; Phospholipase_D-nuclease N-terminal (PF13396; HMM-score: 45.4)
    Pox_A14; Poxvirus virion envelope protein A14 (PF05767; HMM-score: 14.7)
    MFS (CL0015) MFS_Mycoplasma; Mycoplasma MFS transporter (PF07672; HMM-score: 13.5)
    CopD_like (CL0430) UPF0093; Uncharacterised protein family (UPF0093) (PF03653; HMM-score: 13.3)
    no clan defined DUF805; Protein of unknown function (DUF805) (PF05656; HMM-score: 13.1)
    DUF2207_C; Predicted membrane protein (DUF2207) C-terminal domain (PF20990; HMM-score: 12.4)
    DUF3611; Protein of unknown function (DUF3611) (PF12263; HMM-score: 11.6)
    GPCR_A (CL0192) 7tm_3; 7 transmembrane sweet-taste receptor of 3 GCPR (PF00003; HMM-score: 8.7)
    TSPAN_4TM-like (CL0347) DUF4064; Protein of unknown function (DUF4064) (PF13273; HMM-score: 7.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helices: 2
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9987
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0013
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.000898
    • TAT(Tat/SPI): 0.000089
    • LIPO(Sec/SPII): 0.008617
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPFFIGEKTMRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASSTWAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYGTDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEYYTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFASKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAVDALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYTKMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNASDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLAKDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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