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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_1230 [new locus tag: NWMN_RS06950 ]
- pan locus tag?: SAUPAN003683000
- symbol: NWMN_1230
- pan gene symbol?: cls1
- synonym:
- product: phospholipase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_1230 [new locus tag: NWMN_RS06950 ]
- symbol: NWMN_1230
- product: phospholipase
- replicon: chromosome
- strand: +
- coordinates: 1353887..1355395
- length: 1509
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5330732 NCBI
- RefSeq: YP_001332264 NCBI
- BioCyc:
- MicrobesOnline: 3706782 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1501GTGCCTTTCTTTATAGGAGAAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACG
TTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATT
ATCTTTTTAGAAAGAAATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTT
TTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCA
CGCAAATTGAATAAAAACAATGGTAACGTGTTAACGGATTTCGATGGACTTTTAAAACAA
CAAATAGAAAGCTTTGATAAAGGTAATTATGGTACTGATAACAAACAAGTTCAAAAACAT
CATGATTTAGTACGTATGCTTTTGATGGATCAAGATGGTTTTTTAACTGAAAATAATAAA
GTTGATCATTTCATTGATGGAAATGATTTATATGATCAAGTTTTAAAAGATATTAAAAAT
GCAAAAGAATATATCCATTTAGAGTACTATACTTTCGCTTTAGATGGTTTAGGTAAAAGA
ATTTTACATGCTTTAGAAGAAAAATTGAAACAAGGTCTAGAAGTAAAAATATTATATGAT
GATGTTGGATCTAAAAATGTTAAGATGGCAAATTTTGATCATTTTAAATCGTTAGGTGGA
GAAGTTGAAGCATTTTTTGCTTCAAAATTACCGTTATTGAATTTCAGAATGAATAATAGA
AATCATAGAAAAATCATCGTAATCGATGGTCAACTAGGTTATGTCGGAGGATTTAACATT
GGTGATGAATATCTAGGATTAGGAAAATTAGGATATTGGAGAGATACGCATTTACGTATA
CAAGGGGATGCGGTTGATGCACTGCAGTTGCGATTTATTTTAGACTGGAATTCGCAAGCG
CACCGTCCACAATTTGAATATGATGTTAAGTATTTCCCTAAAAAGAACGGACCATTGGGC
AATTCACCAATTCAAATAGCTGCAAGTGGCCCGGCTAGTGACTGGCATCAAATTGAATAC
GGTTATACAAAAATGATTATGAGTGCAAAGAAATCTGTATATTTACAATCACCATATTTC
ATTCCGGATAATTCATATATAAATGCCATTAAAATTGCTGCTAAATCAGGTGTAGATGTA
CATTTAATGATTCCATGTAAGCCAGATCATCCATTAGTATATTGGGCGACATTTTCAAAT
GCCTCTGACTTATTATCAAGTGGTGTTAAAATTTATACGTATGAAAATGGATTTATACAT
TCTAAAATGTGCTTAATTGATGATGAAATCGTATCAGTGGGCACAGCAAATATGGACTTT
AGAAGTTTTGAATTAAATTTTGAAGTAAATGCCTTTGTATATGATGAAAATCTTGCTAAA
GATTTAAGGGTGGCTTATGAACATGATATTACAAAATCAAAACAACTAACCAAAGAATCA
TATGCCAATAGACCGCTGTCTGTTAAATTCAAAGAATCGTTAGCAAAATTAGTTTCGCCA
ATTTTATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_1230 [new locus tag: NWMN_RS06950 ]
- symbol: NWMN_1230
- description: phospholipase
- length: 502
- theoretical pI: 7.81952
- theoretical MW: 57416.6
- GRAVY: -0.136653
⊟Function[edit | edit source]
- reaction: EC 2.7.8.-? ExPASy2 Phosphatidylglycerol = diphosphatidylglycerol + glycerol?
- TIGRFAM: cardiolipin synthase (TIGR04265; EC 2.7.8.-; HMM-score: 822.2)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: PLD (CL0479) PLDc_2; PLD-like domain (PF13091; HMM-score: 145.6)and 7 morePLDc; Phospholipase D Active site motif (PF00614; HMM-score: 51)no clan defined PLDc_N; Phospholipase_D-nuclease N-terminal (PF13396; HMM-score: 45)CopD_like (CL0430) UPF0093; Uncharacterised protein family (UPF0093) (PF03653; HMM-score: 15.5)no clan defined Pox_A14; Poxvirus virion envelope protein A14 (PF05767; HMM-score: 14.7)DUF3611; Protein of unknown function (DUF3611) (PF12263; HMM-score: 13.6)MFS (CL0015) MFS_Mycoplasma; Mycoplasma MFS transporter (PF07672; HMM-score: 13.2)no clan defined DUF2207; Predicted membrane protein (DUF2207) (PF09972; HMM-score: 12.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0.17
- Cytoplasmic Membrane Score: 9.51
- Cellwall Score: 0.16
- Extracellular Score: 0.15
- Internal Helices: 2
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.000898
- TAT(Tat/SPI): 0.000089
- LIPO(Sec/SPII): 0.008617
- predicted transmembrane helices (TMHMM): 2
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MPFFIGEKTMRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASSTWAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYGTDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEYYTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFASKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAVDALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYTKMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNASDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLAKDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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