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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01310
  • pan locus tag?: SAUPAN003683000
  • symbol: SAOUHSC_01310
  • pan gene symbol?: cls1
  • synonym:
  • product: cardiolipin synthetase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01310
  • symbol: SAOUHSC_01310
  • product: cardiolipin synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 1253958..1255439
  • length: 1482
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGCGATTTACATTTTCAAACGATTTAGGAACGTTATTTACTATTATTTTAGCCATTGGA
    TTCATCATTAATTTAGTATTGGCTTTTATTATTATCTTTTTAGAAAGAAATAGGCGTACA
    GCGAGTTCAACTTGGGCATGGCTATTTGTACTTTTTGTCTTACCATTGATTGGTTTTATT
    CTTTACTTGTTTTTTGGTAGAACCGTTTCGGCACGCAAATTGAATAAAAACAATGGTAAC
    GTGTTAACGGATTTCGATGGACTTTTAAAACAACAAATAGAAAGCTTTGATAAAGGTAAT
    TATGGTACTGATAACAAACAAGTTCAAAAACATCATGATTTAGTACGTATGCTTTTGATG
    GATCAAGATGGTTTTTTAACTGAAAATAATAAAGTTGATCATTTCATTGATGGAAATGAT
    TTATATGATCAAGTTTTAAAAGATATTAAAAATGCAAAAGAATATATCCATTTAGAGTAC
    TATACTTTCGCTTTAGATGGTTTAGGTAAAAGAATTTTACATGCTTTAGAAGAAAAATTG
    AAACAAGGTCTAGAAGTAAAAATATTATATGATGATGTTGGATCTAAAAATGTTAAGATG
    GCAAATTTTGATCATTTTAAATCGTTAGGTGGAGAAGTTGAAGCATTTTTTGCTTCAAAA
    TTACCGTTATTGAATTTCAGAATGAATAATAGAAATCATAGAAAAATCATCGTAATCGAT
    GGTCAACTAGGTTATGTCGGAGGATTTAACATTGGTGATGAATATCTAGGATTAGGAAAA
    TTAGGATATTGGAGAGATACGCATTTACGTATACAAGGGGATGCGGTTGATGCACTGCAG
    TTGCGATTTATTTTAGACTGGAATTCGCAAGCGCACCGTCCACAATTTGAATATGATGTT
    AAGTATTTCCCTAAAAAGAACGGACCATTGGGCAATTCACCAATTCAAATAGCTGCAAGT
    GGCCCGGCTAGTGACTGGCATCAAATTGAATACGGTTATACAAAAATGATTATGAGTGCA
    AAGAAATCTGTATATTTACAATCACCATATTTCATTCCGGATAATTCATATATAAATGCC
    ATTAAAATTGCTGCTAAATCAGGTGTAGATGTACATTTAATGATTCCATGTAAGCCAGAT
    CATCCATTAGTATATTGGGCGACATTTTCAAATGCCTCTGACTTATTATCAAGTGGTGTT
    AAAATTTATACGTATGAAAATGGATTTATACATTCTAAAATGTGCTTAATTGATGATGAA
    ATCGTATCAGTGGGCACAGCAAATATGGACTTTAGAAGTTTTGAATTAAATTTTGAAGTA
    AATGCCTTTGTATATGATGAAAATCTTGCTAAAGATTTAAGGGTGGCTTATGAACATGAT
    ATTACAAAATCAAAACAACTAACCAAAGAATCATATGCCAATAGACCGCTGTCTGTTAAA
    TTCAAAGAATCGTTAGCAAAATTAGTTTCGCCAATTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1482

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01310
  • symbol: SAOUHSC_01310
  • description: cardiolipin synthetase
  • length: 493
  • theoretical pI: 7.81981
  • theoretical MW: 56365.4
  • GRAVY: -0.143002

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.8.-?  ExPASy
    2 Phosphatidylglycerol = diphosphatidylglycerol + glycerol?
  • TIGRFAM:
    cardiolipin synthase (TIGR04265; EC 2.7.8.-; HMM-score: 822.3)
  • TheSEED  :
    • Cardiolipin synthetase (EC 2.7.8.-)
    Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Cardiolipin synthetase (EC 2.7.8.-)
  • PFAM:
    PLD (CL0479) PLDc_2; PLD-like domain (PF13091; HMM-score: 145.7)
    and 7 more
    PLDc; Phospholipase D Active site motif (PF00614; HMM-score: 51)
    no clan defined PLDc_N; Phospholipase_D-nuclease N-terminal (PF13396; HMM-score: 45)
    CopD_like (CL0430) UPF0093; Uncharacterised protein family (UPF0093) (PF03653; HMM-score: 15)
    no clan defined Pox_A14; Poxvirus virion envelope protein A14 (PF05767; HMM-score: 14.9)
    MFS (CL0015) MFS_Mycoplasma; Mycoplasma MFS transporter (PF07672; HMM-score: 13.2)
    no clan defined DUF3611; Protein of unknown function (DUF3611) (PF12263; HMM-score: 12.9)
    DUF2207; Predicted membrane protein (DUF2207) (PF09972; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003217
    • TAT(Tat/SPI): 0.000183
    • LIPO(Sec/SPII): 0.029245
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASSTWAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYGTDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEYYTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFASKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAVDALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYTKMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNASDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLAKDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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