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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1308 [new locus tag: NWMN_RS07365 ]
  • pan locus tag?: SAUPAN003810000
  • symbol: dapD
  • pan gene symbol?: dapD
  • synonym:
  • product: tetrahydrodipicolinate acetyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1308 [new locus tag: NWMN_RS07365 ]
  • symbol: dapD
  • product: tetrahydrodipicolinate acetyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1440676..1441395
  • length: 720
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGTACAACATTTAACAGCTGAAGAAATTATTCAATATATAAGTGATGCTAAAAAGTCT
    ACACCAATAAAAGTATATTTAAATGGTAATTTTGAAGGCATCACATATCCAGAAAGTTTT
    AAAGTATTTGGTTCAGAACAATCTAAAGTAATCTTTTGTGAAGCGGATGATTGGAAACCT
    TTTTACGAAGCATATGGTAGTCAATTCGAAGATATAGAAATTGAAATGGATCGTCGCAAT
    TCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTT
    ATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAAT
    ATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGT
    GCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAA
    CCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTT
    ATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCTATTGTTGCAGCTGGCGCGATTGTGACA
    CAAGATGTACCAGCTGGTGCAGTTGTTGCTGGTACACCCTGCAAAGTGATTAAAGCAGCT
    TCTGAAGTACAAGATACTAAAAAAGAGATTGTAGCAGCATTAAGAAAACTGAATGACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1308 [new locus tag: NWMN_RS07365 ]
  • symbol: DapD
  • description: tetrahydrodipicolinate acetyltransferase
  • length: 239
  • theoretical pI: 4.43859
  • theoretical MW: 25232.7
  • GRAVY: 0.133054

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.1.89?  ExPASy
    Tetrahydrodipicolinate N-acetyltransferase Acetyl-CoA + (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + H2O = CoA + L-2-acetamido-6-oxoheptanedioate
  • TIGRFAM:
    2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (TIGR03532; EC 2.3.1.89; HMM-score: 373.5)
    and 16 more
    Metabolism Amino acid biosynthesis Aspartate family 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (TIGR00965; EC 2.3.1.117; HMM-score: 96)
    sugar O-acyltransferase, sialic acid O-acetyltransferase NeuD family (TIGR03570; HMM-score: 89.5)
    phosphonate metabolim protein, transferase hexapeptide repeat family (TIGR03308; HMM-score: 54.1)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 49.4)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 49.4)
    Metabolism Central intermediary metabolism Amino sugars UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 49.4)
    Metabolism Amino acid biosynthesis Serine family serine O-acetyltransferase (TIGR01172; EC 2.3.1.30; HMM-score: 41.3)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase LpxD (TIGR01853; EC 2.3.1.191; HMM-score: 41.1)
    Metabolism Energy metabolism Other phenylacetic acid degradation protein PaaY (TIGR02287; HMM-score: 39.9)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase (TIGR01852; EC 2.3.1.129; HMM-score: 31.7)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR03991; EC 2.3.1.157,2.7.7.23; HMM-score: 27.1)
    Metabolism Central intermediary metabolism Amino sugars UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR03991; EC 2.3.1.157,2.7.7.23; HMM-score: 27.1)
    UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR03992; EC 2.3.1.157,2.7.7.23; HMM-score: 27.1)
    non-ribosomal peptide synthetase terminal domain of unknown function (TIGR02353; HMM-score: 22.3)
    colanic acid biosynthesis acetyltransferase WcaB (TIGR04016; EC 2.3.1.-; HMM-score: 21.2)
    colanic acid biosynthesis acetyltransferase WcaF (TIGR04008; EC 2.3.1.-; HMM-score: 18.6)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined DapH_N; Tetrahydrodipicolinate succinyltransferase N-terminal (PF08503; HMM-score: 108.2)
    and 2 more
    HEXAPEP (CL0536) Hexapep; Bacterial transferase hexapeptide (six repeats) (PF00132; HMM-score: 67.1)
    Hexapep_2; Hexapeptide repeat of succinyl-transferase (PF14602; HMM-score: 50)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.01292
    • TAT(Tat/SPI): 0.000394
    • LIPO(Sec/SPII): 0.002679
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFGSEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIREQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIEPPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPCKVIKAASEVQDTKKEIVAALRKLND

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: L-box (transcription termination) regulon, CodY (repression) regulon
    L-box(RNA)important in Lysine biosynthesis; transcription unit transferred from N315 data RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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