PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1290 [new locus tag: SAUSA300_RS07010 ]
• pan locus tag^?: SAUPAN003810000
• symbol: dapD
• pan gene symbol^?: dapD
• synonym:
• product: tetrahydrodipicolinate acetyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1290 [new locus tag: SAUSA300_RS07010 ]
• symbol: dapD
• product: tetrahydrodipicolinate acetyltransferase
• replicon: chromosome
• strand: +
• coordinates: 1420697..1421416
• length: 720
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915377 NCBI
• RefSeq: YP_493987 NCBI
• BioCyc: see SAUSA300_RS07010
• MicrobesOnline: 1292805 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGTACAACATTTAACAGCTGAAGAAATTATTCAATATATAAGTGATGCTAAAAAGTCT
  ACACCAATAAAAGTATATTTAAATGGTAATTTTGAAGGCATCACATATCCAGAAAGTTTT
  AAAGTATTTGGTTCAGAACAATCTAAAGTAATCTTTTGTGAAGCGGATGATTGGAAACCT
  TTTTACGAAGCATATGGTAGTCAATTCGAAGATATAGAAATTGAAATGGATCGTCGCAAT
  TCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTT
  ATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAAT
  ATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGT
  GCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAA
  CCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTT
  ATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCTATTGTTGCAGCTGGCGCGATTGTGACA
  CAAGATGTACCAGCTGGTGCAGTTGTTGCTGGTACACCTGCAAAAGTGATTAAGCAAGCT
  TCTGAAGTACAAGATACTAAAAAAGAGATTGTAGCAGCATTAAGAAAACTGAATGACTAG
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⊟Protein

⊟General

• locus tag: SAUSA300_1290 [new locus tag: SAUSA300_RS07010 ]
• symbol: DapD
• description: tetrahydrodipicolinate acetyltransferase
• length: 239
• theoretical pI: 4.43859
• theoretical MW: 25257.7
• GRAVY: 0.10795

⊟Function

• reaction:
  EC 2.3.1.89?  ExPASy
  Tetrahydrodipicolinate N-acetyltransferase Acetyl-CoA + (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + H₂O = CoA + L-2-acetamido-6-oxoheptanedioate
  EC 2.3.1.117?  ExPASy
  2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase Succinyl-CoA + (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + H₂O = CoA + N-succinyl-L-2-amino-6-oxoheptanedioate
• TIGRFAM:
  2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (TIGR03532; EC 2.3.1.89; HMM-score: 376.9)
  and 16 more
  Metabolism Amino acid biosynthesis Aspartate family 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (TIGR00965; EC 2.3.1.117; HMM-score: 95.9)
  sugar O-acyltransferase, sialic acid O-acetyltransferase NeuD family (TIGR03570; HMM-score: 91.6)
  phosphonate metabolim protein, transferase hexapeptide repeat family (TIGR03308; HMM-score: 57.4)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 50.8)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 50.8)
  Metabolism Central intermediary metabolism Amino sugars UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 50.8)
  Metabolism Energy metabolism Other phenylacetic acid degradation protein PaaY (TIGR02287; HMM-score: 43.2)
  Metabolism Amino acid biosynthesis Serine family serine O-acetyltransferase (TIGR01172; EC 2.3.1.30; HMM-score: 42.4)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase LpxD (TIGR01853; EC 2.3.1.191; HMM-score: 41.9)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase (TIGR01852; EC 2.3.1.129; HMM-score: 32.9)
  UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR03992; EC 2.3.1.157,2.7.7.23; HMM-score: 27.1)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR03991; EC 2.3.1.157,2.7.7.23; HMM-score: 26.8)
  Metabolism Central intermediary metabolism Amino sugars UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR03991; EC 2.3.1.157,2.7.7.23; HMM-score: 26.8)
  non-ribosomal peptide synthetase terminal domain of unknown function (TIGR02353; HMM-score: 24.3)
  colanic acid biosynthesis acetyltransferase WcaB (TIGR04016; EC 2.3.1.-; HMM-score: 22.8)
  colanic acid biosynthesis acetyltransferase WcaF (TIGR04008; EC 2.3.1.-; HMM-score: 22.1)
• TheSEED  :
  • 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (EC 2.3.1.89)
  Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Lysine Biosynthesis DAP Pathway  2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (EC 2.3.1.89)
• PFAM:
  no clan defined DapH_N; Tetrahydrodipicolinate succinyltransferase N-terminal (PF08503; HMM-score: 108.3)
  and 2 more
  HEXAPEP (CL0536) Hexapep; Bacterial transferase hexapeptide (six repeats) (PF00132; HMM-score: 67.1)
  Hexapep_2; Hexapeptide repeat of succinyl-transferase (PF14602; HMM-score: 50)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.01292
  • TAT(Tat/SPI): 0.000394
  • LIPO(Sec/SPII): 0.002679
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162006 NCBI
• RefSeq: YP_493987 NCBI
• UniProt: Q2FH41 UniProt

⊟Protein sequence

• MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFGSEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIREQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIEPPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIKQASEVQDTKKEIVAALRKLND

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1286 > asd > dapA > dapB > dapD

⊟Regulation

• regulators: L-box (transcription termination) regulon, CodY (repression) regulon

  L-box	(RNA)	important in Lysine biosynthesis; transcription unit transferred from N315 data RegPrecise
  CodY	(TF)	important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications