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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1286 [new locus tag: SAUSA300_RS06990 ]
  • pan locus tag?: SAUPAN003806000
  • symbol: SAUSA300_1286
  • pan gene symbol?: lysC
  • synonym:
  • product: aspartate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1286 [new locus tag: SAUSA300_RS06990 ]
  • symbol: SAUSA300_1286
  • product: aspartate kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 1416804..1418009
  • length: 1206
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGTAACAAGAAGTGTGTTGAAATTTGGCGGATCATCCGTCAGTGATTTTACAAAAATA
    AAAAGGATCGCTGAAATGTTAAAGGAGCGAGTCAATCAAGATGAACAATTAATTGTCGTT
    GTAAGTGCTATGGGTAACACAACAGATCAATTAATGACGAATGTATCAACCTTGACTAAA
    GCACCAAAACAACAAGAACTGGCATTATTATTGACAACCGGAGAGCAACAAACTGTATCT
    TATTTATCAATGGTATTAAATGATATCGGTATGAATGCCAAAGCAATGACTGGCTATCAA
    GCGGGTATTAAAACCATTGGCCATCATTTAAAAAGTAAAATTGCTCAAATTAATCCTCAA
    ACATTTGAACAAGCCTTTCAAGAAAACGATATTTTAGTAGTTGCTGGATTTCAAGGCATC
    AATGAACATCAGGAATTAACAACTTTAGGCAGAGGTGGTTCTGATACGACCGCTGTGGCA
    CTTGCTGTTAGTAATCAAATACCTTGTGAAATTTATACCGACGTTGATGGTGTGTATGCC
    ACTGACCCAAGACTTTTACCAAAGGCTAAACGACTAGACATCGTCTCATATGAAGAAATG
    ATGGAAATGAGCGCTTTAGGTGCTGGTGTACTTGAAACAAGAAGTGTTGAATTAGCTAAA
    AACTATAATATCCCTTTATATTTAGGAAAAACTTTATCGAACGTGAAAGGAACATGGATT
    ATATCAAATGAAGAAATATTAGAGAAAAAAGCAGTTACTGGTGTGGCTTTGGATAAACAT
    ATGATGCATGTAACAATTAGTTATCCCCTACCTGACAATCAGCTACTTACCCAACTATTT
    ACGGAACTTGAAGAAGGTGCTGTAAATGTTGATATGATTTCACAAATCGTCAACTTGGAT
    GGGCTACAACTATCCTTCACGATTAAAGATAGTGATTTTCATCAAATTTCTATGATTCTT
    GAAACATTAAAGAATCAATATGAAGCATTAGCTTATAAAATCAATGAGCATTATGTCAAA
    ATTTCATTAATTGGCTCAGGCATGCGTGATATGTCAGGTGTGGCATCAAAAGCATTTTTG
    ACATTAATTGAAAATAATATACCTTTCTACCAAACAACAACATCTGAAATAAGTATTTCA
    TACGTCATTGATGATTTTAATGGGCAACAAGCGGTAGAAAAACTATATGACGCATTTAAC
    ATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1206

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1286 [new locus tag: SAUSA300_RS06990 ]
  • symbol: SAUSA300_1286
  • description: aspartate kinase
  • length: 401
  • theoretical pI: 4.70763
  • theoretical MW: 44356.5
  • GRAVY: -0.0526185

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.2.4?  ExPASy
    Aspartate kinase ATP + L-aspartate = ADP + 4-phospho-L-aspartate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aspartate family aspartate kinase (TIGR00657; EC 2.7.2.4; HMM-score: 354)
    Metabolism Amino acid biosynthesis Aspartate family aspartate kinase, monofunctional class (TIGR00656; EC 2.7.2.4; HMM-score: 331)
    and 4 more
    Metabolism Amino acid biosynthesis Aspartate family putative Pyrococcus aspartate kinase subunit (TIGR02078; EC 2.7.2.4; HMM-score: 105.8)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions UMP kinase (TIGR02075; EC 2.7.4.22; HMM-score: 36.5)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions putative uridylate kinase (TIGR02076; EC 2.7.4.-; HMM-score: 31.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine pyrophosphokinase (TIGR01378; EC 2.7.6.2; HMM-score: 16.1)
  • TheSEED  :
    • Aspartokinase (EC 2.7.2.4)
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Lysine Biosynthesis DAP Pathway  Aspartokinase (EC 2.7.2.4)
    and 1 more
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Threonine and Homoserine Biosynthesis  Aspartokinase (EC 2.7.2.4)
  • PFAM:
    no clan defined AA_kinase; Amino acid kinase family (PF00696; HMM-score: 148.1)
    and 3 more
    ACT (CL0070) ACT_7; ACT domain (PF13840; HMM-score: 27)
    no clan defined TPK_catalytic; Thiamin pyrophosphokinase, catalytic domain (PF04263; HMM-score: 20.3)
    E-set (CL0159) Alpha_adaptinC2; Adaptin C-terminal domain (PF02883; HMM-score: 14.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004219
    • TAT(Tat/SPI): 0.000769
    • LIPO(Sec/SPII): 0.000608
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAMGNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGMNAKAMTGYQAGIKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVALAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVELAKNYNIPLYLGKTLSNVKGTWIISNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLLTQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKINEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAVEKLYDAFNI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: L-box (transcription termination) regulon, CodY (repression) regulon
    L-box(RNA)important in Lysine biosynthesis; regulatory site identified based on RegPrecise data for N315 RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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