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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1287 [new locus tag: SAUSA300_RS06995 ]
  • pan locus tag?: SAUPAN003807000
  • symbol: asd
  • pan gene symbol?: asd
  • synonym:
  • product: aspartate semialdehyde dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1287 [new locus tag: SAUSA300_RS06995 ]
  • symbol: asd
  • product: aspartate semialdehyde dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 1418073..1419062
  • length: 990
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGACAAAGTTAGCAGTTGTGGGTGCAACAGGATTAGTAGGTACAAAAATGTTGGAGACA
    TTAAATCGTAAAAATATTCCTTTCGATGAATTAGTATTATTTTCATCAGCACGTTCTGCA
    GGGCAAGAAGTTGAATTTCAAGGAAAAACATATACAGTTCAAGAATTAACTGATGCTCGT
    GCAAGTGAACATTTCGATTATGTATTAATGAGTGCTGGTGGCGGTACAAGCGAACACTTT
    GCCCCACTTTTTGAAAAAGCTGGTGCAATCGTTATAGACAATTCAAGTCAATGGCGTATG
    GCAGAAGATATTGATTTAATCGTTCCGGAAGTCAATGAACCTACATTTACAAGAGGTATC
    ATTGCCAATCCAAACTGCTCTACGATTCAATCTGTTGTACCTCTAAAAGTATTGCAAGAT
    GCTTATGGTTTAAAACGAGTGGCATATACAACATATCAAGCTGTATCAGGTTCAGGGATG
    AAAGGTAAGAAAGATTTAGCTGAAGGTGTAAATGGTAAAGCACCAGAAGCATATCCACAT
    CCAATTTATAATAATGTGTTACCGCATATTGATGTGTTTTTAGAAAACGGATATACAAAA
    GAAGAACAAAAAATGATTGATGAGACGAGAAAAATTTTAAATGCGCCAGACTTAAAAGTA
    ACAGCAACATGCGCACGTGTGCCTGTTCAAGATAGTCATAGTGTTGAAATTGATGTAACG
    CTTGACAAAGAAACAACAGCAGAAGATATTAAAGCGTTATTTGATCAAGATGACCGCGTT
    GTTTTAGTAGACAATCCAGAGAACAATGAATATCCAATGGCAATCAATTCTACTAATAAA
    GATGAAGTGTTTGTTGGCCGTATACGTAGAGATGATTCATTAGAAAATACTTTCCATGTA
    TGGTGTACATCAGACAATTTATTAAAAGGTGCTGCATTAAATGCTGTACAAGTATTGGAA
    CAAGTTATGCGTTTAAAAGGAGCGAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    990

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1287 [new locus tag: SAUSA300_RS06995 ]
  • symbol: Asd
  • description: aspartate semialdehyde dehydrogenase
  • length: 329
  • theoretical pI: 4.64802
  • theoretical MW: 36282.7
  • GRAVY: -0.293009

⊟Function[edit | edit source]

  • reaction:
    EC 1.2.1.11?  ExPASy
    Aspartate-semialdehyde dehydrogenase L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aspartate family aspartate-semialdehyde dehydrogenase (TIGR01296; EC 1.2.1.11; HMM-score: 379.1)
    and 9 more
    Metabolism Amino acid biosynthesis Aspartate family aspartate-semialdehyde dehydrogenase (TIGR00978; EC 1.2.1.11; HMM-score: 126.2)
    Metabolism Amino acid biosynthesis Aspartate family aspartate-semialdehyde dehydrogenase (TIGR01745; EC 1.2.1.11; HMM-score: 83.2)
    Metabolism Amino acid biosynthesis Glutamate family N-acetyl-gamma-glutamyl-phosphate reductase (TIGR01850; EC 1.2.1.38; HMM-score: 58.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine erythrose-4-phosphate dehydrogenase (TIGR01532; EC 1.2.1.72; HMM-score: 30)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis glyceraldehyde-3-phosphate dehydrogenase, type I (TIGR01534; EC 1.2.1.-; HMM-score: 27.6)
    Metabolism Amino acid biosynthesis Glutamate family N-acetyl-gamma-glutamyl-phosphate reductase (TIGR01851; EC 1.2.1.38; HMM-score: 27.6)
    glyceraldehyde-3-phosphate dehydrogenase, type II (TIGR01546; EC 1.2.1.59; HMM-score: 16.4)
    Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 14.3)
    Genetic information processing Mobile and extrachromosomal element functions Plasmid functions integrating conjugative element protein, PFL_4703 family (TIGR03746; HMM-score: 13.6)
  • TheSEED  :
    • Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11)
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Lysine Biosynthesis DAP Pathway  Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11)
    and 1 more
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Threonine and Homoserine Biosynthesis  Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11)
  • PFAM:
    GADPH_aa-bio_dh (CL0139) Semialdhyde_dhC; Semialdehyde dehydrogenase, dimerisation domain (PF02774; HMM-score: 154.4)
    and 6 more
    NADP_Rossmann (CL0063) Semialdhyde_dh; Semialdehyde dehydrogenase, NAD binding domain (PF01118; HMM-score: 100)
    GADPH_aa-bio_dh (CL0139) Gp_dh_C; Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain (PF02800; HMM-score: 30.2)
    NADP_Rossmann (CL0063) Ldh_1_N; lactate/malate dehydrogenase, NAD binding domain (PF00056; HMM-score: 16.1)
    Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 14.7)
    no clan defined TGT_C1; C1 domain of tRNA-guanine transglycosylase dimerisation (PF14809; HMM-score: 13.2)
    DUF2895; Protein of unknown function (DUF2895) (PF11444; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.033175
    • TAT(Tat/SPI): 0.000893
    • LIPO(Sec/SPII): 0.004506
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEVEFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAEDIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGMKGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDLKVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAINSTNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: L-box (transcription termination) regulon, CodY (repression) regulon
    L-box(RNA)important in Lysine biosynthesis; transcription unit transferred from N315 data RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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