PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1350 [new locus tag: NWMN_RS07615 ]
• pan locus tag^?: SAUPAN003894000
• symbol: NWMN_1350
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Additional information (user-provided)

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1350 [new locus tag: NWMN_RS07615 ]
• symbol: NWMN_1350
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1513570..1514448
• length: 879
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331727 NCBI
• RefSeq: YP_001332384 NCBI
• BioCyc:
• MicrobesOnline: 3706902 MicrobesOnline

⊟Phenotype

⊟Additional information (user-provided)

⊟DNA sequence

• 1
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  481
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  661
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  841
  
  ATGCCTAATAAAATATTACTTGTAGATGGTATGGCGCTATTATTTAGACATTTCTATGCT
  ACAAGTCTTCATAAACAATTTATGTACAATTCACAAGGTGAACCTACAAATGGAATACAA
  GGATTTGTGCGTCATATCTTTTCGGCAATACATGAAATACGCCCTACACATGTAGCTGTA
  TGTTGGGATATGGGACAATCAACTTTTAGAAATGATATGTTTGATGGTTATAAGCAAAAT
  CGTTCTGCACCACCAGAAGAATTGATACCACAATTTGATTATGTTAAAGAAATTTCAGAG
  CAATTTGGCTTTGTAAATATTGGCGTTAAAAACTATGAAGCGGATGATGTTATAGGTACA
  TTAGCACAACAATATTCAACTGATAACGATGTCTATATTATTACGGGCGACAAAGATTTA
  CTGCAATGTATTAATGACAATGTTGAAGTTTGGCTAATTAAAAAAGGTTTTAACATTTAT
  AATAGATATACATTACATCGTTTTAACGAAGAATATGCCCTTGAACCACAACAACTAATC
  GATATTAAAGCGTTTATGGGTGATACAGCAGATGGATATGCGGGTGTTAAAGGTATTGGC
  GAGAAAACGGCTATTAAGTTAATTCAGCAATATCAAAGCGTTGAAAATGTGGTTGAAAAC
  ATTGATGCATTATCAGCTGGGCAACGTAATAAGATTAATGACAATTTAGATGAACTATAT
  TTATCGAAGCGACTGGCTGAAATTCACACACAAGTACCAATTGATAGTGAAGCGCTTTTT
  GAGAAAATGTCATTTGCAACAACGCTTAATCATATTTTATCAATTTGTAACGAACATGAA
  TTACATGTTTCAGGAAAATATATTTCATCCCATTTTTAA
  60
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⊟Protein

⊟General

• locus tag: NWMN_1350 [new locus tag: NWMN_RS07615 ]
• symbol: NWMN_1350
• description: hypothetical protein
• length: 292
• theoretical pI: 5.04103
• theoretical MW: 33401.5
• GRAVY: -0.321918

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase I (TIGR00593; EC 2.7.7.7; HMM-score: 266.7)
  and 2 more
  flap structure-specific endonuclease (TIGR03674; EC 3.1.-.-; HMM-score: 32)
  Metabolism Amino acid biosynthesis Pyruvate family threonine dehydratase (TIGR02079; EC 4.3.1.19; HMM-score: 14.8)
• TheSEED  :
  • DNA polymerase I (EC 2.7.7.7)
  DNA Metabolism DNA repair DNA Repair Base Excision  DNA polymerase I (EC 2.7.7.7)
  and 1 more
  DNA Metabolism DNA replication DNA-replication  DNA polymerase I (EC 2.7.7.7)
• PFAM:
  PIN (CL0280) 5_3_exonuc_N; 5'-3' exonuclease, N-terminal resolvase-like domain (PF02739; HMM-score: 178.8)
  and 5 more
  5_3_exonuc_C (CL0464) 5_3_exonuc; 5'-3' exonuclease, C-terminal SAM fold (PF01367; HMM-score: 96.5)
  HHH (CL0198) HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 16.9)
  PIN (CL0280) NYN_YacP; YacP-like NYN domain (PF05991; HMM-score: 15.2)
  HHH (CL0198) HHH_2; Helix-hairpin-helix motif (PF12826; HMM-score: 13.5)
  DNA_pol_lambd_f; Fingers domain of DNA polymerase lambda (PF10391; HMM-score: 13)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9856
  • Cytoplasmic Membrane Score: 0.0072
  • Cell wall & surface Score: 0.0007
  • Extracellular Score: 0.0065
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 0.83
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.016391
  • TAT(Tat/SPI): 0.001089
  • LIPO(Sec/SPII): 0.003369
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221562 NCBI
• RefSeq: YP_001332384 NCBI
• UniProt: A0A0H3K8J5 UniProt

⊟Additional information (user-provided)

⊟Protein sequence

• MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGEPTNGIQGFVRHIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQFGFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIYNRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVVENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSICNEHELHVSGKYISSHF

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1350 < NWMN_1351

⊟Regulation

• regulator:

⊟Additional information (user-provided)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Additional information (user-provided)

⊟Other information (user-provided)

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications