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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1332 [new locus tag: SAUSA300_RS07260 ]
  • pan locus tag?: SAUPAN003894000
  • symbol: SAUSA300_1332
  • pan gene symbol?: —
  • synonym:
  • product: putative 5'-3' exonuclease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1332 [new locus tag: SAUSA300_RS07260 ]
  • symbol: SAUSA300_1332
  • product: putative 5'-3' exonuclease
  • replicon: chromosome
  • strand: -
  • coordinates: 1494103..1494981
  • length: 879
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGCCTAATAAAATATTACTTGTAGATGGTATGGCGCTATTATTTAGACATTTCTATGCT
    ACAAGTCTTCATAAACAATTTATGTACAATTCACAAGGTGAACCTACAAATGGAATACAA
    GGATTTGTGCGTCATATCTTTTCGGCAATACATGAAATACGCCCTACACATGTAGCTGTA
    TGTTGGGATATGGGACAATCAACTTTTAGAAATGATATGTTTGATGGTTATAAGCAAAAT
    CGTTCTGCACCACCAGAAGAATTGATACCACAATTTGATTATGTTAAAGAAATTTCAGAG
    CAATTTGGCTTTGTAAATATTGGCGTTAAAAACTATGAAGCGGATGATGTTATAGGTACA
    TTAGCACAACAATATTCAACTGATAACGATGTCTATATTATTACGGGCGACAAAGATTTA
    CTGCAATGTATTAATGACAATGTTGAAGTTTGGCTAATTAAAAAAGGTTTTAACATTTAT
    AATAGATATACATTACATCGTTTTAACGAAGAATATGCCCTTGAACCACAACAACTAATC
    GATATTAAAGCGTTTATGGGTGATACAGCAGATGGATATGCGGGTGTTAAAGGTATTGGC
    GAGAAAACGGCTATTAAGTTAATTCAGCAATATCAAAGCGTTGAAAATGTGGTTGAAAAC
    ATTGATGCATTATCAGCTGGGCAACGTAATAAGATTAATGACAATTTAGATGAACTATAT
    TTATCGAAGCGACTGGCTGAAATTCACACACAAGTACCAATTGATAGTGAAGCGCTTTTT
    GAGAAAATGTCATTTGCAACAACGCTTAATCATATTTTATCAATTTGTAACGAACATGAA
    TTACATGTTTCAGGAAAATATATTTCATCCCATTTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    879

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1332 [new locus tag: SAUSA300_RS07260 ]
  • symbol: SAUSA300_1332
  • description: putative 5'-3' exonuclease
  • length: 292
  • theoretical pI: 5.04103
  • theoretical MW: 33401.5
  • GRAVY: -0.321918

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase I (TIGR00593; EC 2.7.7.7; HMM-score: 266.7)
    and 2 more
    flap structure-specific endonuclease (TIGR03674; EC 3.1.-.-; HMM-score: 32)
    Metabolism Amino acid biosynthesis Pyruvate family threonine dehydratase (TIGR02079; EC 4.3.1.19; HMM-score: 14.8)
  • TheSEED  :
    • DNA polymerase I (EC 2.7.7.7)
    DNA Metabolism DNA repair DNA Repair Base Excision  DNA polymerase I (EC 2.7.7.7)
    and 1 more
    DNA Metabolism DNA replication DNA-replication  DNA polymerase I (EC 2.7.7.7)
  • PFAM:
    PIN (CL0280) 5_3_exonuc_N; 5'-3' exonuclease, N-terminal resolvase-like domain (PF02739; HMM-score: 176.5)
    and 3 more
    5_3_exonuc_C (CL0464) 5_3_exonuc; 5'-3' exonuclease, C-terminal SAM fold (PF01367; HMM-score: 94)
    HHH (CL0198) HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 16.3)
    HHH_2; Helix-hairpin-helix motif (PF12826; HMM-score: 15.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016391
    • TAT(Tat/SPI): 0.001089
    • LIPO(Sec/SPII): 0.003369
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGEPTNGIQGFVRHIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQFGFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIYNRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVVENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSICNEHELHVSGKYISSHF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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