PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1435 [new locus tag: NWMN_RS08100 ]
• pan locus tag^?: SAUPAN004108000
• symbol: NWMN_1435
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1435 [new locus tag: NWMN_RS08100 ]
• symbol: NWMN_1435
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1606169..1606750
• length: 582
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330851 NCBI
• RefSeq: YP_001332469 NCBI
• BioCyc:
• MicrobesOnline: 3706987 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGAAAAAATTGGTTTCAATTGTTGGCGCAACATTATTGTTAGCTGGATGTGGATCACAA
  AATTTAGCACCATTAGAAGAAAAAACAACAGATTTAAGAGAAGATAATCATCAACTCAAA
  CTAGATATTCAAGAACTTAATCAACAAATTAGTGATTCTAAATCTAAAATTAAAGGGCTT
  GAAAAGGATAAAGAAAACAGTAAAAAAACTGCATCTAATAATACGAAAATTAAATTGATG
  AATGTTACATCAACATACTACGACAAAGTTGCTAAAGCTTTGAAATCCTATAACGATATT
  GAGAAAGATGTAAGTAAAAACAAAGGCGATAAGAATGTTCAATCGAAATTAAATCAAATT
  TCTAATGATATTCAAAGTGCTCACACTTCATACAAAGATGCTATCGATGGTTTATCACTT
  AGTGATGATGATAAAAAAACGTCTAAAAATATCGATAAATTAAACTCTGATTTGAATCAT
  GCATTTGATGATATTAAAAATGGCTATCAAAATAAAGATAAAAAACAACTTACAAAAGGA
  CAACAAGCGTTGTCAAAATTAAACTTAAATGCAAAATCATGA
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  582
  

⊟Protein

⊟General

• locus tag: NWMN_1435 [new locus tag: NWMN_RS08100 ]
• symbol: NWMN_1435
• description: hypothetical protein
• length: 193
• theoretical pI: 9.8465
• theoretical MW: 21472
• GRAVY: -0.999482

⊟Function

• TIGRFAM:
  SH3 domain protein (TIGR04211; HMM-score: 14.2)
  and 6 more
  Cellular processes Cellular processes Pathogenesis type III secretion apparatus needle protein (TIGR02105; HMM-score: 11.3)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type III secretion apparatus needle protein (TIGR02105; HMM-score: 11.3)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 11.2)
  Genetic information processing Mobile and extrachromosomal element functions Plasmid functions integrating conjugative element protein, PFL_4705 family (TIGR03752; HMM-score: 7.3)
  phage lysis regulatory protein, LysB family (TIGR03495; HMM-score: 5.8)
  Metabolism Energy metabolism Photosynthesis photosystem II protein PsbQ (TIGR03042; HMM-score: 5.1)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  OML_zippers (CL0590) LPP; Lipoprotein leucine-zipper (PF04728; HMM-score: 16.3)
  no clan defined Lzipper-MIP1; Leucine-zipper of ternary complex factor MIP1 (PF14389; HMM-score: 14.8)
  IncA; IncA protein (PF04156; HMM-score: 14.2)
  Syntaxin-6_N; Syntaxin 6, N-terminal (PF09177; HMM-score: 13.9)
  DUF4763; Domain of unknown function (DUF4763) (PF15960; HMM-score: 13.4)
  LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 13.2)
  and 18 more
  HTH (CL0123) Mnd1; Mnd1 family (PF03962; HMM-score: 12.5)
  no clan defined DUF5344; Family of unknown function (DUF5344) (PF17279; HMM-score: 12.3)
  TMCO5; TMCO5 family (PF14992; HMM-score: 11.8)
  YabB; Initiation control protein YabA (PF06156; HMM-score: 11.6)
  PRKG1_interact; cGMP-dependent protein kinase interacting domain (PF15898; HMM-score: 11.1)
  LppaM (CL0421) LPAM_2; Prokaryotic lipoprotein-attachment site (PF13627; HMM-score: 10.8)
  no clan defined DUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 10.7)
  BCLiA (CL0551) APG6; Autophagy protein Apg6 (PF04111; HMM-score: 9.8)
  Vps51 (CL0295) COG2; COG (conserved oligomeric Golgi) complex component, COG2 (PF06148; HMM-score: 9.1)
  no clan defined Cnn_1N; Centrosomin N-terminal motif 1 (PF07989; HMM-score: 9.1)
  Jnk-SapK_ap_N; JNK_SAPK-associated protein-1 (PF09744; HMM-score: 8.1)
  FlaC_arch; Flagella accessory protein C (FlaC) (PF05377; HMM-score: 7.9)
  SNARE-fusion (CL0445) Syntaxin_2; Syntaxin-like protein (PF14523; HMM-score: 7.6)
  bZIP (CL0018) bZIP_1; bZIP transcription factor (PF00170; HMM-score: 7.4)
  FKBP (CL0487) Rotamase; PPIC-type PPIASE domain (PF00639; HMM-score: 7.4)
  no clan defined Ax_dynein_light; Axonemal dynein light chain (PF10211; HMM-score: 7.2)
  GrpE; GrpE (PF01025; HMM-score: 6.5)
  Spc7; Spc7 kinetochore protein (PF08317; HMM-score: 5.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helices: 0
• LocateP: Lipid anchored
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: 0
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: LLAGCGS
• SignalP: Signal peptide LIPO(Sec/SPII) length 16 aa
  • SP(Sec/SPI): 0.000578
  • TAT(Tat/SPI): 0.000055
  • LIPO(Sec/SPII): 0.999143
  • Cleavage Site: CS pos: 16-17. LAG-CG. Pr: 0.9997
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221647 NCBI
• RefSeq: YP_001332469 NCBI
• UniProt: A0A0H3K8Q2 UniProt

⊟Protein sequence

• MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEKDVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNHAFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1435 > NWMN_1436

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications