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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1566 [new locus tag: NWMN_RS08780 ]
  • pan locus tag?: SAUPAN004287000
  • symbol: hemA
  • pan gene symbol?: hemA
  • synonym:
  • product: glutamyl-tRNA reductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1566 [new locus tag: NWMN_RS08780 ]
  • symbol: hemA
  • product: glutamyl-tRNA reductase
  • replicon: chromosome
  • strand: -
  • coordinates: 1732366..1733712
  • length: 1347
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCATTTTATTGCAATTAGTATAAATCATCGCACAGCTGATGTTGCACTAAGAGAGCAA
    GTTGCTTTTAGAGATGATGCCTTACGAATTGCCCATGAAGATTTATATGAAACTAAATCT
    ATTTTAGAAAATGTCATATTATCAACATGTAATCGAACTGAAGTATATGCTGTTGTTGAT
    CAAATTCACACAGGTCGTTACTATATTCAACGATTTCTAGCTCGTGCATTTGGATTTGAA
    GTAGATGATATTAAAGCAATGTCAGAAGTAAAAGTGGGGGACGAAGCAGTAGAACATTTA
    TTGCGTGTCACTTCTGGTTTAGATTCAATCGTACTTGGAGAAACTCAAATTTTAGGTCAA
    ATAAGAGATGCATTTTTCTTAGCGCAAAGCACAGGTACGACAGGAACAATTTTTAATCAT
    CTATTTAAACAGGCAATTACTTTTGCAAAAAGAGCACATAATGAAACAGATATAGCTGAT
    AATGCTGTAAGTGTGTCTTATGCTGCGGTCGAGTTGGCGAAAAAAGTATTTGGCAAATTG
    AAAAGTAAGCAAGCTATCATTATTGGTGCAGGGGAAATGAGTGAATTATCACTATTAAAT
    CTTCTTGGTTCTGGAATTACTGATATTACAGTAGTAAATAGAACAATTGAAAATGCTATG
    AAATTAGCAGCAAAGCATCAAGTGAAATATGATGAACTATCATCATTACCAAATTTACTT
    GAAAGTGCAGATATTGTGATTAGTTCAACGAGTGCACAATCTTATATCATTACAAATGAA
    ATGATAGAAAGAATTGCAGAAAATAGAAAGCAAGATTCACTAGTATTGATTGATATTGCA
    GTTCCTCGAGATATTGAACCTGGTATTAGTGCCATCACAAACATCTTTAATTATGATGTT
    GATGACTTAAAAGGTTTAGTTGATGCAAACTTACGTGAGCGACAATTAGCGGCTGCAACA
    ATTTCGGAACAAATTCCTGCAGAAATACATGCACACAATGAGTGGATTAGTATGTTGGGT
    GTTGTTCCAGTGATTAGAGCTTTACGTGAAAAAGCTATGGCAATTCAAGCAGAAACGATG
    GACAGTATTGATCGTAAATTGCCAGGGTTAAGCGAAAGAGAACGTAAAATTATATCTAAA
    CATACAAAAAGTATCATCAATCAAATGTTGAAAGATCCTATTAAACAGGCCAAAGAATTA
    AGTAGTGATAAGAAAAGTAATGAAAAATTAGAGCTATTTCAAAATATATTTGATATCGAA
    GCTGAATGTCCTCATGAGCAAGCGAAGCAACAAAAAGAAAGCAAGGTCAAGGAAATTTCA
    GCGCGACGTATCTTTAGTTTTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1347

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1566 [new locus tag: NWMN_RS08780 ]
  • symbol: HemA
  • description: glutamyl-tRNA reductase
  • length: 448
  • theoretical pI: 5.81316
  • theoretical MW: 50099
  • GRAVY: -0.148884

⊟Function[edit | edit source]

  • reaction:
    EC 1.2.1.70?  ExPASy
    Glutamyl-tRNA reductase L-glutamate 1-semialdehyde + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamyl-tRNA reductase (TIGR01035; EC 1.2.1.70; HMM-score: 449.5)
    and 5 more
    Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate dehydrogenase (TIGR00507; EC 1.1.1.25; HMM-score: 27.4)
    Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate-5-dehydrogenase (TIGR01809; EC 1.1.1.25; HMM-score: 24.4)
    ectoine utilization protein EutC (TIGR02992; HMM-score: 22.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin siroheme synthase, N-terminal domain (TIGR01470; HMM-score: 15.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis adenylyltransferase ThiF (TIGR02356; EC 2.7.7.73; HMM-score: 12.7)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 153.1)
    no clan defined GlutR_N; Glutamyl-tRNAGlu reductase, N-terminal domain (PF05201; HMM-score: 144.6)
    and 9 more
    GlutR_dimer; Glutamyl-tRNAGlu reductase, dimerisation domain (PF00745; HMM-score: 72.4)
    NADP_Rossmann (CL0063) OCD_Mu_crystall; Ornithine cyclodeaminase/mu-crystallin family (PF02423; HMM-score: 22.9)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 22.8)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 19.6)
    THF_DHG_CYH_C; Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain (PF02882; HMM-score: 15.8)
    AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 13.1)
    IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 13.1)
    ThiF; ThiF family (PF00899; HMM-score: 12.6)
    PELOTA (CL0101) RbsD_FucU; RbsD / FucU transport protein family (PF05025; HMM-score: 10.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004119
    • TAT(Tat/SPI): 0.000347
    • LIPO(Sec/SPII): 0.001196
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILENVILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLRVTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIADNAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIENAMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVLIDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNEWISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKDPIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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