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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_1564 [new locus tag: NWMN_RS08770 ]
- pan locus tag?: SAUPAN004285000
- symbol: hemC
- pan gene symbol?: hemC
- synonym:
- product: porphobilinogen deaminase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_1564 [new locus tag: NWMN_RS08770 ]
- symbol: hemC
- product: porphobilinogen deaminase
- replicon: chromosome
- strand: -
- coordinates: 1730561..1731487
- length: 927
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5331988 NCBI
- RefSeq: YP_001332598 NCBI
- BioCyc:
- MicrobesOnline: 3707116 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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901ATGCGTAAATTAGTCGTTGGCTCCAGAAGAAGTAAATTAGCTTTAACACAAAGCCAGCAA
TTTATTGATAAATTAAAAGCTGTCGAGCCAAATCTAGAAATTGAAATTAAAGAAATTGTC
ACGAAAGGCGATCGTATAGTAGATAAACAATTGTCTAAAGTCGGAGGCAAAGGCTTATTT
GTTAAAGAAATACAACATGAACTTTTTGAAAAAAATATCGATATGGCAATACACTCGCTT
AAAGACGTACCAAGTGTAATTCCGGAAGGTTTAACATTAGGTTGTATCCCTGATAGAGAA
TTACCTTTTGATGCGTATATTTCTAAAACACATACACCACTATCCCAATTGCCAGAAGGC
AGTATTATTGGTACTAGTTCATTACGTCGTGGTGCACAAATATTATCTAAGTATCCTAAT
TTAGAGATTAAATGGATTAGAGGTAATATAGATACACGATTAGAAAAGTTACAAACTGAA
GATTATGATGCGATTATTTTAGCTGCAGCTGGTTTAAGAAGAATGGGCTGGTCAGATGAT
ATTGTAACATCTTATCTTGATAGAGATACATTGTTACCTGCAATCGGACAAGGTGCTTTA
GGGATAGAATGTCGTAGTGACGATGAAGAACTATTAACATTATTAAGCAAAGTACATAAT
GATGAGGTTGCAAAATGTGTGACTGCTGAACGAACGTTTTTAGCAGAAATGGATGGTAGT
TGTCAGGTGCCAATTGCAGGATATGCTACAATCTCAGATCAAAACGAAATCGAATTTACA
GGTTTAATTATGACCCCAGATGGTAAAGAACGATTTGAATATACAATGAACGGAACAGAT
CCGGTTGAGTTAGGCAAAACAGTGAGTAACAAATTAAAAGAGCAAGGTGCTTATGAAATT
ATAAAACGCTTAAATGAACAACATTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_1564 [new locus tag: NWMN_RS08770 ]
- symbol: HemC
- description: porphobilinogen deaminase
- length: 308
- theoretical pI: 5.04703
- theoretical MW: 34354.1
- GRAVY: -0.313312
⊟Function[edit | edit source]
- reaction: EC 2.5.1.61? ExPASyHydroxymethylbilane synthase 4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3
- TIGRFAM: Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin hydroxymethylbilane synthase (TIGR00212; EC 2.5.1.61; HMM-score: 352.8)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: PBP (CL0177) Porphobil_deam; Porphobilinogen deaminase, dipyromethane cofactor binding domain (PF01379; HMM-score: 248.8)and 1 moreno clan defined Porphobil_deamC; Porphobilinogen deaminase, C-terminal domain (PF03900; HMM-score: 83.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: dipyrromethane
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.005228
- TAT(Tat/SPI): 0.000756
- LIPO(Sec/SPII): 0.000883
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGDRIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELPFDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTEDYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKVHNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQNEIEFTGLIMTPDGKERFEYTMNGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
NWMN_1605 (ackA) acetate kinase [1] (data from MRSA252) NWMN_1142 (acpP) acyl carrier protein [1] (data from MRSA252) NWMN_0577 (adh1) alcohol dehydrogenase [1] (data from MRSA252) NWMN_2131 (adk) adenylate kinase [1] (data from MRSA252) NWMN_0372 (ahpC) alkyl hydroperoxide reductase subunit C [1] (data from MRSA252) NWMN_0113 (aldA) aldehyde dehydrogenase-like protein [1] (data from MRSA252) NWMN_2534 (arcA) arginine deiminase [1] (data from MRSA252) NWMN_2533 (arcB) ornithine carbamoyltransferase [1] (data from MRSA252) NWMN_0579 (argS) arginyl-tRNA synthetase [1] (data from MRSA252) NWMN_1365 (asnC) asparaginyl-tRNA synthetase [1] (data from MRSA252) NWMN_1587 (citC) isocitrate dehydrogenase [1] (data from MRSA252) NWMN_1588 (citZ) citrate synthase [1] (data from MRSA252) NWMN_1313 (cspA) major cold-shock protein CspA [1] (data from MRSA252) NWMN_1987 (ddl) D-alanyl-alanine synthetase A [1] (data from MRSA252) NWMN_2042 (deoD) purine nucleoside phosphorylase [1] (data from MRSA252) NWMN_1483 (dnaK) molecular chaperone DnaK [1] (data from MRSA252) NWMN_0002 (dnaN) DNA polymerase III subunit beta [1] (data from MRSA252) NWMN_0082 (dra) deoxyribose-phosphate aldolase [1] (data from MRSA252) NWMN_1433 (efp) elongation factor P [1] (data from MRSA252) NWMN_0745 (eno) phosphopyruvate hydratase [1] (data from MRSA252) NWMN_0551 (eutD) phosphotransacetylase [1] (data from MRSA252) NWMN_1141 (fabG) 3-oxoacyl-[acyl-carrier protein] reductase [1] (data from MRSA252) NWMN_2029 (fbaA) fructose-bisphosphate aldolase [1] (data from MRSA252) NWMN_1625 (fhs) formate--tetrahydrofolate ligase [1] (data from MRSA252) NWMN_0932 (folD) bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase [1] (data from MRSA252) NWMN_1169 (frr) ribosome recycling factor [1] (data from MRSA252) NWMN_1096 (ftsZ) cell division protein FtsZ [1] (data from MRSA252) NWMN_0509 (fus) elongation factor G [1] (data from MRSA252) NWMN_0741 (gapA) glyceraldehyde 3-phosphate dehydrogenase 1 [1] (data from MRSA252) NWMN_1580 (gapB) glyceraldehyde 3-phosphate dehydrogenase 2 [1] (data from MRSA252) NWMN_1838 (gatA) aspartyl/glutamyl-tRNA amidotransferase subunit A [1] (data from MRSA252) NWMN_1837 (gatB) aspartyl/glutamyl-tRNA amidotransferase subunit B [1] (data from MRSA252) NWMN_1440 (gcvPA) glycine dehydrogenase subunit 1 [1] (data from MRSA252) NWMN_1441 (gcvT) glycine cleavage system aminomethyltransferase T [1] (data from MRSA252) NWMN_2056 (glmS) glucosamine--fructose-6-phosphate aminotransferase [1] (data from MRSA252) NWMN_1217 (glnA) glutamine synthetase [1] (data from MRSA252) NWMN_1417 (gnd) 6-phosphogluconate dehydrogenase [1] (data from MRSA252) NWMN_2315 (gpmA) phosphoglyceromutase [1] (data from MRSA252) NWMN_0381 (guaA) GMP synthase [1] (data from MRSA252) NWMN_0380 (guaB) inosine-5'-monophosphate dehydrogenase [1] (data from MRSA252) NWMN_0828 (gudB) NAD-specific glutamate dehydrogenase [1] (data from MRSA252) NWMN_1756 (hemL) glutamate-1-semialdehyde aminotransferase [1] (data from MRSA252) NWMN_0516 (ilvE) branched-chain amino acid aminotransferase [1] (data from MRSA252) NWMN_1246 (katA) catalase [1] (data from MRSA252) NWMN_2499 (ldh) L-lactate dehydrogenase 2 [1] (data from MRSA252) NWMN_0176 (ldh1) L-lactate dehydrogenase [1] (data from MRSA252) NWMN_0479 (lysS) lysyl-tRNA synthetase [1] (data from MRSA252) NWMN_1334 (msrB) methionine sulfoxide reductase B [1] (data from MRSA252) NWMN_2028 (murZ) UDP-N-acetylglucosamine 1-carboxyvinyltransferase [1] (data from MRSA252) NWMN_2446 (mvaS) 3-hydroxy-3-methylglutaryl coenzyme A synthase [1] (data from MRSA252) NWMN_0701 (nrdF) ribonucleotide-diphosphate reductase subunit beta [1] (data from MRSA252) NWMN_1681 (pckA) phosphoenolpyruvate carboxykinase [1] (data from MRSA252) NWMN_0961 (pdhC) branched-chain alpha-keto acid dehydrogenase subunit E2 [1] (data from MRSA252) NWMN_0962 (pdhD) dihydrolipoamide dehydrogenase [1] (data from MRSA252) NWMN_2040 (pdp) pyrimidine-nucleoside phosphorylase [1] (data from MRSA252) NWMN_1593 (pfk) 6-phosphofructokinase [1] (data from MRSA252) NWMN_0162 (pflB) formate acetyltransferase [1] (data from MRSA252) NWMN_0833 (pgi) glucose-6-phosphate isomerase [1] (data from MRSA252) NWMN_0742 (pgk) phosphoglycerate kinase [1] (data from MRSA252) NWMN_0744 (pgm) phosphoglyceromutase [1] (data from MRSA252) NWMN_0959 (phdA) pyruvate dehydrogenase E1 component, alpha subunit [1] (data from MRSA252) NWMN_1592 (pykA) pyruvate kinase [1] (data from MRSA252) NWMN_1112 (pyrC) dihydroorotase [1] (data from MRSA252) NWMN_0500 (rplA) 50S ribosomal protein L1 [1] (data from MRSA252) NWMN_2149 (rplB) 50S ribosomal protein L2 [1] (data from MRSA252) NWMN_2152 (rplC) 50S ribosomal protein L3 [1] (data from MRSA252) NWMN_2151 (rplD) 50S ribosomal protein L4 [1] (data from MRSA252) NWMN_2140 (rplE) 50S ribosomal protein L5 [1] (data from MRSA252) NWMN_2137 (rplF) 50S ribosomal protein L6 [1] (data from MRSA252) NWMN_0501 (rplJ) 50S ribosomal protein L10 [1] (data from MRSA252) NWMN_0499 (rplK) 50S ribosomal protein L11 [1] (data from MRSA252) NWMN_0502 (rplL) 50S ribosomal protein L7/L12 [1] (data from MRSA252) NWMN_2120 (rplM) 50S ribosomal protein L13 [1] (data from MRSA252) NWMN_2133 (rplO) 50S ribosomal protein L15 [1] (data from MRSA252) NWMN_2125 (rplQ) 50S ribosomal protein L17 [1] (data from MRSA252) NWMN_2136 (rplR) 50S ribosomal protein L18 [1] (data from MRSA252) NWMN_1151 (rplS) 50S ribosomal protein L19 [1] (data from MRSA252) NWMN_1572 (rplT) 50S ribosomal protein L20 [1] (data from MRSA252) NWMN_1549 (rplU) 50S ribosomal protein L21 [1] (data from MRSA252) NWMN_2147 (rplV) 50S ribosomal protein L22 [1] (data from MRSA252) NWMN_2150 (rplW) 50S ribosomal protein L23 [1] (data from MRSA252) NWMN_2141 (rplX) 50S ribosomal protein L24 [1] (data from MRSA252) NWMN_0464 (rplY) 50S ribosomal protein L25/general stress protein Ctc [1] (data from MRSA252) NWMN_1547 (rpmA) 50S ribosomal protein L27 [1] (data from MRSA252) NWMN_2024 (rpmE2) 50S ribosomal protein L31 type B [1] (data from MRSA252) NWMN_1573 (rpmI) 50S ribosomal protein L35 [1] (data from MRSA252) NWMN_0504 (rpoB) DNA-directed RNA polymerase subunit beta [1] (data from MRSA252) NWMN_0505 (rpoC) DNA-directed RNA polymerase subunit beta' [1] (data from MRSA252) NWMN_2032 (rpoE) DNA-directed RNA polymerase subunit delta [1] (data from MRSA252) NWMN_1385 (rpsA) 30S ribosomal protein S1 [1] (data from MRSA252) NWMN_1166 (rpsB) 30S ribosomal protein S2 [1] (data from MRSA252) NWMN_2146 (rpsC) 30S ribosomal protein S3 [1] (data from MRSA252) NWMN_1613 (rpsD) 30S ribosomal protein S4 [1] (data from MRSA252) NWMN_2135 (rpsE) 30S ribosomal protein S5 [1] (data from MRSA252) NWMN_0357 (rpsF) 30S ribosomal protein S6 [1] (data from MRSA252) NWMN_0508 (rpsG) 30S ribosomal protein S7 [1] (data from MRSA252) NWMN_2138 (rpsH) 30S ribosomal protein S8 [1] (data from MRSA252) NWMN_2119 (rpsI) 30S ribosomal protein S9 [1] (data from MRSA252) NWMN_2153 (rpsJ) 30S ribosomal protein S10 [1] (data from MRSA252) NWMN_2127 (rpsK) 30S ribosomal protein S11 [1] (data from MRSA252) NWMN_2128 (rpsM) 30S ribosomal protein S13 [1] (data from MRSA252) NWMN_1182 (rpsO) 30S ribosomal protein S15 [1] (data from MRSA252) NWMN_1148 (rpsP) 30S ribosomal protein S16 [1] (data from MRSA252) NWMN_2143 (rpsQ) 30S ribosomal protein S17 [1] (data from MRSA252) NWMN_2148 (rpsS) 30S ribosomal protein S19 [1] (data from MRSA252) NWMN_1972 (rsbV) anti-sigma B factor antagonist [1] (data from MRSA252) NWMN_1456 (sodA) superoxide dismutase Mn/Fe family protein [1] (data from MRSA252) NWMN_0077 (sodM) superoxide dismutase [1] (data from MRSA252) NWMN_0055 (spa) immunoglobulin G binding protein A precursor (protein A) [1] (data from MRSA252) NWMN_0461 (spoVG) regulatory protein SpoVG [1] (data from MRSA252) NWMN_1325 (sucB) dihydrolipoamide succinyltransferase [1] (data from MRSA252) NWMN_1155 (sucC) succinyl-CoA synthetase subunit beta [1] (data from MRSA252) NWMN_0785 (sufC) FeS assembly ATPase SufC [1] (data from MRSA252) NWMN_0786 (sufD) FeS assembly protein SufD [1] (data from MRSA252) NWMN_0543 (thiD) phosphomethylpyrimidine kinase [1] (data from MRSA252) NWMN_1576 (thrS) threonyl-tRNA synthetase [1] (data from MRSA252) NWMN_1569 (tig) trigger factor [1] (data from MRSA252) NWMN_1254 (tkt) transketolase [1] (data from MRSA252) NWMN_0743 (tpiA) triosephosphate isomerase [1] (data from MRSA252) NWMN_1057 (trxA) thioredoxin [1] (data from MRSA252) NWMN_0732 (trxB) thioredoxin reductase [1] (data from MRSA252) NWMN_1167 (tsf) elongation factor Ts [1] (data from MRSA252) NWMN_0510 (tufA) elongation factor Tu [1] (data from MRSA252) NWMN_2016 (upp) uracil phosphoribosyltransferase [1] (data from MRSA252) NWMN_0355 GTP-dependent nucleic acid-binding protein EngD [1] (data from MRSA252) NWMN_0428 ABC transporter substrate-binding protein [1] (data from MRSA252) NWMN_0443 hypothetical protein [1] (data from MRSA252) NWMN_0475 cysteine synthase-like protein [1] (data from MRSA252) NWMN_0481 pyridoxal biosynthesis lyase PdxS [1] (data from MRSA252) NWMN_0482 glutamine amidotransferase subunit PdxT [1] (data from MRSA252) NWMN_0512 2-amino-3-ketobutyrate coenzyme A ligase [1] (data from MRSA252) NWMN_0533 hypothetical protein [1] (data from MRSA252) NWMN_0638 hypothetical protein [1] (data from MRSA252) NWMN_0721 sigma 54 modulation protein [1] (data from MRSA252) NWMN_0774 hypothetical protein [1] (data from MRSA252) NWMN_0776 glycine cleavage system protein H [1] (data from MRSA252) NWMN_0811 hypothetical protein [1] (data from MRSA252) NWMN_0839 fumarylacetoacetate hydrolase family protein [1] (data from MRSA252) NWMN_0854 3-oxoacyl-(acyl-carrier-protein) synthase II [1] (data from MRSA252) NWMN_0870 oligoendopeptidase F [1] (data from MRSA252) NWMN_0881 enoyl-(acyl carrier protein) reductase [1] (data from MRSA252) NWMN_0885 hypothetical protein [1] (data from MRSA252) NWMN_0949 phosphocarrier protein HPr [1] (data from MRSA252) NWMN_1086 hypothetical protein [1] (data from MRSA252) NWMN_1263 aconitate hydratase [1] (data from MRSA252) NWMN_1275 4-oxalocrotonate tautomerase [1] (data from MRSA252) NWMN_1303 ABC transporter ATP-binding protein [1] (data from MRSA252) NWMN_1333 phosphotransferase system glucose-specific IIA component [1] (data from MRSA252) NWMN_1382 DNA-binding protein HU [1] (data from MRSA252) NWMN_1600 universal stress protein family protein [1] (data from MRSA252) NWMN_1604 universal stress protein family protein [1] (data from MRSA252) NWMN_1643 D-alanine aminotransferase [1] (data from MRSA252) NWMN_1644 dipeptidase PepV [1] (data from MRSA252) NWMN_1672 putative translaldolase [1] (data from MRSA252) NWMN_1831 ferritin [1] (data from MRSA252) NWMN_1857 putative manganese-dependent inorganic pyrophosphatase [1] (data from MRSA252) NWMN_2026 aldehyde dehydrogenase family protein [1] (data from MRSA252) NWMN_2043 hypothetical protein [1] (data from MRSA252) NWMN_2074 hypothetical protein [1] (data from MRSA252) NWMN_2086 alkaline shock protein 23 [1] (data from MRSA252) NWMN_2454 1-pyrroline-5-carboxylate dehydrogenase [1] (data from MRSA252) NWMN_2503 fructose-1,6-bisphosphate aldolase [1] (data from MRSA252) NWMN_2504 malate:quinone oxidoreductase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.000 1.001 1.002 1.003 1.004 1.005 1.006 1.007 1.008 1.009 1.010 1.011 1.012 1.013 1.014 1.015 1.016 1.017 1.018 1.019 1.020 1.021 1.022 1.023 1.024 1.025 1.026 1.027 1.028 1.029 1.030 1.031 1.032 1.033 1.034 1.035 1.036 1.037 1.038 1.039 1.040 1.041 1.042 1.043 1.044 1.045 1.046 1.047 1.048 1.049 1.050 1.051 1.052 1.053 1.054 1.055 1.056 1.057 1.058 1.059 1.060 1.061 1.062 1.063 1.064 1.065 1.066 1.067 1.068 1.069 1.070 1.071 1.072 1.073 1.074 1.075 1.076 1.077 1.078 1.079 1.080 1.081 1.082 1.083 1.084 1.085 1.086 1.087 1.088 1.089 1.090 1.091 1.092 1.093 1.094 1.095 1.096 1.097 1.098 1.099 1.100 1.101 1.102 1.103 1.104 1.105 1.106 1.107 1.108 1.109 1.110 1.111 1.112 1.113 1.114 1.115 1.116 1.117 1.118 1.119 1.120 1.121 1.122 1.123 1.124 1.125 1.126 1.127 1.128 1.129 1.130 1.131 1.132 1.133 1.134 1.135 1.136 1.137 1.138 1.139 1.140 1.141 1.142 1.143 1.144 1.145 1.146 1.147 1.148 1.149 1.150 1.151 1.152 1.153 1.154 1.155 1.156 1.157 1.158 1.159 1.160 1.161 1.162 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)