PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1619 [new locus tag: SAUSA300_RS08825 ]
• pan locus tag^?: SAUPAN004287000
• symbol: hemA
• pan gene symbol^?: hemA
• synonym:
• product: glutamyl-tRNA reductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1619 [new locus tag: SAUSA300_RS08825 ]
• symbol: hemA
• product: glutamyl-tRNA reductase
• replicon: chromosome
• strand: -
• coordinates: 1772364..1773710
• length: 1347
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913554 NCBI
• RefSeq: YP_494314 NCBI
• BioCyc: see SAUSA300_RS08825
• MicrobesOnline: 1293134 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGCATTTTATTGCAATTAGTATAAATCATCGCACAGCTGATGTTGCACTAAGAGAGCAA
  GTTGCTTTTAGAGATGATGCCTTACGAATTGCCCATGAAGATTTATATGAAACTAAATCT
  ATTTTAGAAAATGTCATATTATCAACATGTAATCGAACTGAAGTATATGCTGTTGTTGAT
  CAAATTCACACAGGTCGTTACTATATTCAACGATTTCTAGCTCGTGCATTTGGATTTGAA
  GTAGATGATATTAAAGCAATGTCAGAAGTAAAAGTGGGGGACGAAGCAGTAGAACATTTA
  TTGCGTGTCACTTCTGGTTTAGATTCAATCGTACTTGGAGAAACTCAAATTTTAGGTCAA
  ATAAGAGATGCATTTTTCTTAGCGCAAAGCACAGGTACGACAGGAACAATTTTTAATCAT
  CTATTTAAACAGGCAATTACTTTTGCAAAAAGAGCACATAATGAAACAGATATAGCTGAT
  AATGCTGTAAGTGTGTCTTATGCTGCGGTCGAGTTGGCGAAAAAAGTATTTGGCAAATTG
  AAAAGTAAGCAAGCTATCATTATTGGTGCAGGGGAAATGAGTGAATTATCACTATTAAAT
  CTTCTTGGTTCTGGAATTACTGATATTACAGTAGTAAATAGAACAATTGAAAATGCTATG
  AAATTAGCAGCAAAGCATCAAGTGAAATATGATGAACTATCATCATTACCAAATTTACTT
  GAAAGTGCAGATATTGTGATTAGTTCAACGAGTGCACAATCTTATATCATTACAAATGAA
  ATGATAGAAAGAATTGCAGAAAATAGAAAGCAAGATTCACTAGTATTGATTGATATTGCA
  GTTCCTCGAGATATTGAACCTGGTATTAGTGCCATCACAAACATCTTTAATTATGATGTT
  GATGACTTAAAAGGTTTAGTTGATGCAAACTTACGTGAGCGACAATTAGCGGCTGCAACA
  ATTTCGGAACAAATTCCTGCAGAAATACATGCACACAATGAGTGGATTAGTATGTTGGGT
  GTTGTTCCAGTGATTAGAGCTTTACGTGAAAAAGCTATGGCAATTCAAGCAGAAACGATG
  GACAGTATTGATCGTAAATTGCCAGGGTTAAGCGAAAGAGAACGTAAAATTATATCTAAA
  CATACAAAAAGTATCATCAATCAAATGTTGAAAGATCCTATTAAACAGGCCAAAGAATTA
  AGTAGTGATAAGAAAAGTAATGAAAAATTAGAGCTATTTCAAAATATATTTGATATCGAA
  GCTGAATGTCCTCATGAGCAAGCGAAGCAACAAAAAGAAAGCAAGGTCAAGGAAATTTCA
  GCGCGACGTATCTTTAGTTTTGAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1619 [new locus tag: SAUSA300_RS08825 ]
• symbol: HemA
• description: glutamyl-tRNA reductase
• length: 448
• theoretical pI: 5.81316
• theoretical MW: 50099
• GRAVY: -0.148884

⊟Function

• reaction:
  EC 1.2.1.70?  ExPASy
  Glutamyl-tRNA reductase L-glutamate 1-semialdehyde + NADP⁺ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamyl-tRNA reductase (TIGR01035; EC 1.2.1.70; HMM-score: 449.5)
  and 5 more
  Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate dehydrogenase (TIGR00507; EC 1.1.1.25; HMM-score: 27.4)
  Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate-5-dehydrogenase (TIGR01809; EC 1.1.1.25; HMM-score: 24.4)
  ectoine utilization protein EutC (TIGR02992; HMM-score: 22.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin siroheme synthase, N-terminal domain (TIGR01470; HMM-score: 15.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis adenylyltransferase ThiF (TIGR02356; EC 2.7.7.73; HMM-score: 12.7)
• TheSEED  :
  • Glutamyl-tRNA reductase (EC 1.2.1.70)
  Cofactors, Vitamins, Prosthetic Groups, Pigments Tetrapyrroles CPO analysis  Glutamyl-tRNA reductase (EC 1.2.1.70)
• PFAM:
  NADP_Rossmann (CL0063) Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 153.1)
  no clan defined GlutR_N; Glutamyl-tRNAGlu reductase, N-terminal domain (PF05201; HMM-score: 144.6)
  and 9 more
  GlutR_dimer; Glutamyl-tRNAGlu reductase, dimerisation domain (PF00745; HMM-score: 72.4)
  NADP_Rossmann (CL0063) OCD_Mu_crystall; Ornithine cyclodeaminase/mu-crystallin family (PF02423; HMM-score: 22.9)
  F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 22.8)
  NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 19.6)
  THF_DHG_CYH_C; Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain (PF02882; HMM-score: 15.8)
  AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 13.1)
  IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 13.1)
  ThiF; ThiF family (PF00899; HMM-score: 12.6)
  PELOTA (CL0101) RbsD_FucU; RbsD / FucU transport protein family (PF05025; HMM-score: 10.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004119
  • TAT(Tat/SPI): 0.000347
  • LIPO(Sec/SPII): 0.001196
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160833 NCBI
• RefSeq: YP_494314 NCBI
• UniProt: Q2FG64 UniProt

⊟Protein sequence

• MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILENVILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLRVTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIADNAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIENAMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVLIDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNEWISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKDPIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: hemL < hemB < hemD < hemC < hemX < hemA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications