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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1618 [new locus tag: SAUSA300_RS08820 ]
  • pan locus tag?: SAUPAN004286000
  • symbol: hemX
  • pan gene symbol?: hemX
  • synonym:
  • product: hemA concentration negative effector hemX

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1618 [new locus tag: SAUSA300_RS08820 ]
  • symbol: hemX
  • product: hemA concentration negative effector hemX
  • replicon: chromosome
  • strand: -
  • coordinates: 1771527..1772342
  • length: 816
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGCAAGAAAACCTGTTTATTCGATTCAATGAAATTATATTATTAATATACTTAATCAGT
    ATCATTTGCTATTTTTATGATTTTGTACAAAAAAGTCATAAGATTAGAAGTTTAGGCATA
    TATTTATTGGGGATTGTTTGGGTTTTACAAACAATCTCTTTATCTATTTTTATTATACAA
    ACTAGACATATTCCATTAGGGTCTATTTCAGATGTATTCTATACTTTAAGTTGGCTGATT
    ATTTCAATCTCTTTAATTCTTAACCTTATCAAAGTTTTGAATTTCTCTGTGTTTTTCTTG
    AATTTAATAGGGTTAACACTTTTAGGGATGAATACATTTCAACCGACACATTATACTAAT
    AAGGTTCAGAAAATTGCTGTTGTAGACGAGTTACTGTTAGTCCATATCGGTCTAGCTGTG
    TTAAGTTATGTCTTCTTTGCCTTAGCTTTTGTCAATGCATTACTGTATATCATGCAATAC
    CGTAATTTGAAGGAAAAACGTTTTGATCAAAAATACTTTAGAATTGGTAGCGTTGCTACA
    CTTGAATCAATTGTGTTTTACTCAACGTTAAGTGGGTGGATAATTCTCATATTTAGTATT
    ATTTTAGGTACACAGTGGGGTGTAATTTCAGTTGGTGAACGTATATTCATTGACCCGAAG
    GTAATACTCTCTTCAATTATTACAGTATTATATGGAAGTTATATTTTACTTAGAATCAAC
    AAATGGTTGAATTCAAGATATTTAATATACTATAACATTATTTTATTTTGCTTAAATATG
    ATTAATTTATTTTTTGCAACACATTTTGTGAATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    816

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1618 [new locus tag: SAUSA300_RS08820 ]
  • symbol: HemX
  • description: hemA concentration negative effector hemX
  • length: 271
  • theoretical pI: 9.6883
  • theoretical MW: 31546.6
  • GRAVY: 0.934686

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair cytochrome c-type biogenesis protein CcsB (TIGR03144; HMM-score: 48.4)
    Metabolism Energy metabolism Electron transport cytochrome c-type biogenesis protein CcsB (TIGR03144; HMM-score: 48.4)
  • TheSEED  :
    • HemX protein, negative effector of steady-state concentration of glutamyl-tRNA reductase
    CBSS-196164.1.peg.461  HemX protein, negative effector of steady-state concentration of glutamyl-tRNA reductase
    and 1 more
    Experimental tye  HemX protein, negative effector of steady-state concentration of glutamyl-tRNA reductase
  • PFAM:
    2heme_cytochrom (CL0328) Cytochrom_C_asm; Cytochrome C assembly protein (PF01578; HMM-score: 69.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.000866
    • TAT(Tat/SPI): 0.000092
    • LIPO(Sec/SPII): 0.001471
  • predicted transmembrane helices (TMHMM): 8

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLGIVWVLQTISLSIFIIQTRHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNLIGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQYRNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFIDPKVILSSIITVLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]