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NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_01775
- pan locus tag?: SAUPAN004286000
- symbol: SAOUHSC_01775
- pan gene symbol?: hemX
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_01775
- symbol: SAOUHSC_01775
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 1674730..1675545
- length: 816
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3919693 NCBI
- RefSeq: YP_500280 NCBI
- BioCyc: G1I0R-1649 BioCyc
- MicrobesOnline: 1290194 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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781ATGCAAGAAAACCTGTTTATTCGATTCAATGAAATTATATTATTAATATACTTAATCAGT
ATCATTTGCTATTTTTATGATTTTGTACAAAAAAGTCATAAGATTAGAAGTTTAGGCATA
TATTTATTGGGGATTGTTTGGGTTTTACAAACAATCTCTTTATCTATTTTTATTATACAA
ACTAGACATATTCCATTAGGGTCTATTTCAGATGTATTCTATACTTTAAGTTGGCTGATT
ATTTCAATCTCTTTAATTCTTAACCTTATCAAAGTTTTGAATTTCTCTGTGTTTTTCTTG
AATTTAATAGGGTTAACACTTTTAGGGATGAATACATTTCAACCGACACATTATACTAAT
AAGGTTCAGAAAATTGCTGTTGTAGACGAGTTACTGTTAGTCCATATCGGTCTAGCTGTG
TTAAGTTATGTCTTCTTTGCCTTAGCTTTTGTCAATGCATTACTGTATATCATGCAATAC
CGTAATTTGAAGGAAAAACGTTTTGATCAAAAATACTTTAGAATTGGTAGCGTTGCTACA
CTTGAATCAATTGTGTTTTACTCAACGTTAAGTGGGTGGATAATTCTCATATTTAGTATT
ATTTTAGGTACACAGTGGGGTGTAATTTCAGTTGGTGAACGTATATTCATTGACCCGAAG
GTAATACTCTCTTCAATTATTACAGTATTATATGGAAGTTATATTTTACTTAGAATCAAC
AAATGGTTGAATTCAAGATATTTAATATACTATAACATTATTTTATTTTGCTTAAATATG
ATTAATTTATTTTTTGCAACACATTTTGTGAATTGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_01775
- symbol: SAOUHSC_01775
- description: hypothetical protein
- length: 271
- theoretical pI: 9.6883
- theoretical MW: 31546.6
- GRAVY: 0.934686
⊟Function[edit | edit source]
- TIGRFAM: Protein fate Protein modification and repair cytochrome c-type biogenesis protein CcsB (TIGR03144; HMM-score: 48.4)Energy metabolism Electron transport cytochrome c-type biogenesis protein CcsB (TIGR03144; HMM-score: 48.4)
- TheSEED :
- HemX protein, negative effector of steady-state concentration of glutamyl-tRNA reductase
CBSS-196164.1.peg.461 HemX protein, negative effector of steady-state concentration of glutamyl-tRNA reductaseand 1 more - PFAM: 2heme_cytochrom (CL0328) Cytochrom_C_asm; Cytochrome C assembly protein (PF01578; HMM-score: 69.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 8
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.000866
- TAT(Tat/SPI): 0.000092
- LIPO(Sec/SPII): 0.001471
- predicted transmembrane helices (TMHMM): 8
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLGIVWVLQTISLSIFIIQTRHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNLIGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQYRNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFIDPKVILSSIITVLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SAOUHSC_01771 < SAOUHSC_01772 < SAOUHSC_01773 < hemC < SAOUHSC_01775 < hemApredicted SigA promoter [1] : SAOUHSC_01771 < SAOUHSC_01772 < SAOUHSC_01773 < hemC < SAOUHSC_01775 < hemApredicted SigA promoter [1] : SAOUHSC_01771 < SAOUHSC_01772 < SAOUHSC_01773 < hemC < SAOUHSC_01775 < hemA < S701 < engB < S702
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [1] Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 1.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)