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NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01775
  • pan locus tag?: SAUPAN004286000
  • symbol: SAOUHSC_01775
  • pan gene symbol?: hemX
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01775
  • symbol: SAOUHSC_01775
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1674730..1675545
  • length: 816
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGCAAGAAAACCTGTTTATTCGATTCAATGAAATTATATTATTAATATACTTAATCAGT
    ATCATTTGCTATTTTTATGATTTTGTACAAAAAAGTCATAAGATTAGAAGTTTAGGCATA
    TATTTATTGGGGATTGTTTGGGTTTTACAAACAATCTCTTTATCTATTTTTATTATACAA
    ACTAGACATATTCCATTAGGGTCTATTTCAGATGTATTCTATACTTTAAGTTGGCTGATT
    ATTTCAATCTCTTTAATTCTTAACCTTATCAAAGTTTTGAATTTCTCTGTGTTTTTCTTG
    AATTTAATAGGGTTAACACTTTTAGGGATGAATACATTTCAACCGACACATTATACTAAT
    AAGGTTCAGAAAATTGCTGTTGTAGACGAGTTACTGTTAGTCCATATCGGTCTAGCTGTG
    TTAAGTTATGTCTTCTTTGCCTTAGCTTTTGTCAATGCATTACTGTATATCATGCAATAC
    CGTAATTTGAAGGAAAAACGTTTTGATCAAAAATACTTTAGAATTGGTAGCGTTGCTACA
    CTTGAATCAATTGTGTTTTACTCAACGTTAAGTGGGTGGATAATTCTCATATTTAGTATT
    ATTTTAGGTACACAGTGGGGTGTAATTTCAGTTGGTGAACGTATATTCATTGACCCGAAG
    GTAATACTCTCTTCAATTATTACAGTATTATATGGAAGTTATATTTTACTTAGAATCAAC
    AAATGGTTGAATTCAAGATATTTAATATACTATAACATTATTTTATTTTGCTTAAATATG
    ATTAATTTATTTTTTGCAACACATTTTGTGAATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    816

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_01775
  • symbol: SAOUHSC_01775
  • description: hypothetical protein
  • length: 271
  • theoretical pI: 9.6883
  • theoretical MW: 31546.6
  • GRAVY: 0.934686

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair cytochrome c-type biogenesis protein CcsB (TIGR03144; HMM-score: 48.4)
    Metabolism Energy metabolism Electron transport cytochrome c-type biogenesis protein CcsB (TIGR03144; HMM-score: 48.4)
  • TheSEED  :
    • HemX protein, negative effector of steady-state concentration of glutamyl-tRNA reductase
    CBSS-196164.1.peg.461  HemX protein, negative effector of steady-state concentration of glutamyl-tRNA reductase
    and 1 more
    Experimental tye  HemX protein, negative effector of steady-state concentration of glutamyl-tRNA reductase
  • PFAM:
    2heme_cytochrom (CL0328) Cytochrom_C_asm; Cytochrome C assembly protein (PF01578; HMM-score: 69.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.000866
    • TAT(Tat/SPI): 0.000092
    • LIPO(Sec/SPII): 0.001471
  • predicted transmembrane helices (TMHMM): 8

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLGIVWVLQTISLSIFIIQTRHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNLIGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQYRNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFIDPKVILSSIITVLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. 1.0 1.1 1.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]