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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1615 [new locus tag: SAUSA300_RS08805 ]
  • pan locus tag?: SAUPAN004283000
  • symbol: hemB
  • pan gene symbol?: hemB
  • synonym:
  • product: delta-aminolevulinic acid dehydratase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1615 [new locus tag: SAUSA300_RS08805 ]
  • symbol: hemB
  • product: delta-aminolevulinic acid dehydratase
  • replicon: chromosome
  • strand: -
  • coordinates: 1768892..1769866
  • length: 975
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAATTTGATAGACATAGAAGATTGAGATCATCAGCGACAATGAGAGATATGGTTAGA
    GAGAATCATGTAAGAAAAGAAGATTTAATATATCCAATTTTTGTAGTTGAAAAAGACGAT
    GTGAAAAAAGAAATTAAGTCATTGCCAGGTGTATACCAAATCAGTTTGAATTTACTTGAA
    AGTGAATTAAAAGAAGCTTATGACTTAGGCATACGTGCCATTATGTTTTTCGGTGTTCCA
    AACTCAAAAGATGATATAGGTACTGGTGCATACATTCACGATGGTGTTATTCAACAGGCA
    ACACGTATTGCTAAAAAAATGTATGATGACTTATTAATTGTTGCAGACACTTGTTTATGT
    GAATATACTGATCATGGTCATTGTGGCGTGATTGATGACCATACACATGACGTTGACAAT
    GATAAATCATTGCCACTACTTGTTAAAACAGCAATTTCTCAAGTGGAAGCTGGTGCTGAT
    ATTATTGCGCCAAGTAATATGATGGATGGTTTTGTTGCTGAAATTCGTCGTGGATTAGAT
    GAAGCCGGCTATTACAATATTCCTATAATGAGTTATGGTGTCAAGTATGCATCAAGTTTC
    TTTGGACCTTTTAGAGATGCAGCAGATTCAGCGCCATCATTTGGGGATAGAAAAACGTAT
    CAGATGGACCCTGCTAACCGTTTGGAAGCACTTCGTGAATTAGAAAGTGATCTTAAAGAA
    GGGTGCGACATGATGATTGTTAAACCTGCTCTAAGTTATTTAGATATAGTTCGAGATGTT
    AAAAATCATACGAATGTTCCAGTTGTTGCATATAATGTGAGTGGAGAATATAGTATGACT
    AAAGCAGCGGCACAAAATGGTTGGATAGATGAAGAACGTGTCGTTATGGAACAAATGGTT
    TCAATGAAACGTGCAGGTGCTGATATGATTATTACGTATTTTGCAAAGGACATTTGTCGC
    TATTTAGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    975

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1615 [new locus tag: SAUSA300_RS08805 ]
  • symbol: HemB
  • description: delta-aminolevulinic acid dehydratase
  • length: 324
  • theoretical pI: 5.03483
  • theoretical MW: 36582.6
  • GRAVY: -0.283025

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.1.24?  ExPASy
    Porphobilinogen synthase 2 5-aminolevulinate = porphobilinogen + 2 H2O
  • TIGRFAM:
  • TheSEED  :
    • Porphobilinogen synthase (EC 4.2.1.24)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Tetrapyrroles CPO analysis  Porphobilinogen synthase (EC 4.2.1.24)
  • PFAM:
    TIM_barrel (CL0036) ALAD; Delta-aminolevulinic acid dehydratase (PF00490; HMM-score: 484.7)
    and 1 more
    no clan defined HMA; Heavy-metal-associated domain (PF00403; HMM-score: 14.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001992
    • TAT(Tat/SPI): 0.000419
    • LIPO(Sec/SPII): 0.00056
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKEIKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATRIAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGADIIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRKTYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGEYSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.000 1.001 1.002 1.003 1.004 1.005 1.006 1.007 1.008 1.009 1.010 1.011 1.012 1.013 1.014 1.015 1.016 1.017 1.018 1.019 1.020 1.021 1.022 1.023 1.024 1.025 1.026 1.027 1.028 1.029 1.030 1.031 1.032 1.033 1.034 1.035 1.036 1.037 1.038 1.039 1.040 1.041 1.042 1.043 1.044 1.045 1.046 1.047 1.048 1.049 1.050 1.051 1.052 1.053 1.054 1.055 1.056 1.057 1.058 1.059 1.060 1.061 1.062 1.063 1.064 1.065 1.066 1.067 1.068 1.069 1.070 1.071 1.072 1.073 1.074 1.075 1.076 1.077 1.078 1.079 1.080 1.081 1.082 1.083 1.084 1.085 1.086 1.087 1.088 1.089 1.090 1.091 1.092 1.093 1.094 1.095 1.096 1.097 1.098 1.099 1.100 1.101 1.102 1.103 1.104 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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