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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2491 [new locus tag: SAUSA300_RS13840 ]
  • pan locus tag?: SAUPAN006217000
  • symbol: SAUSA300_2491
  • pan gene symbol?: rocA
  • synonym:
  • product: 1-pyrroline-5-carboxylate dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2491 [new locus tag: SAUSA300_RS13840 ]
  • symbol: SAUSA300_2491
  • product: 1-pyrroline-5-carboxylate dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 2693539..2695083
  • length: 1545
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGTAGTAGAATTTAAAAATGAACCTGGTTACGATTTTTCAGTCCAAGAAAATGTGGAT
    ATGTTTAAGAAAGCATTGAAAGACGTGGAAAAAGAATTAGGTCAAGACATACCATTAGTG
    ATTAATGGAGAGAAGATTTTCAAAGATGACAAAATTAAATCAATCAATCCTGCAGACACA
    TCACAAGTGATTGCAAATGCATCAAAGGCAACAAAACAAGATGTTGAAGATGCGTTTAAA
    GCAGCAAACGAAGCTTATAAATCTTGGAAAACATGGTCAGCAAATGATCGTGCAGAGCTT
    ATGTTACGTGTTTCGGCAATTATTCGTCGTCGAAAAGCGGAAATTGCAGCAATTATGGTT
    TATGAAGCAGGTAAGCCATGGGATGAAGCAGTTGGTGATGCAGCTGAAGGTATTGATTTT
    ATTGAATACTATGCACGTTCAATGATGGACTTGGCTCAAGGTAAGCCAGTACTTGATCGT
    GAAGGTGAACACAATAAATATTTCTATAAATCAATTGGTACAGGTGTTACAATTCCGCCG
    TGGAACTTCCCGTTTGCGATTATGGCTGGTACAACTTTAGCACCAGTTGTGGCAGGTAAT
    ACAGTGTTATTGAAACCTGCTGAGGATACACCTTATATCGCTTATAAATTAATGGAAATT
    TTAGAAGAAGCAGGATTACCAAAAGGCGTAGTTAACTTTGTTCCTGGTGATCCGAAAGAA
    ATTGGTGATTACTTAGTTGATCATAAAGATACGCATTTCGTTACATTTACAGGATCACGT
    GCGACAGGTACTAGAATTTATGAACGTAGTGCCGTTGTTCAAGAAGGTCAAAACTTCTTG
    AAACGTGTTATCGCTGAAATGGGTGGTAAAGATGCCATTGTAGTTGATGAAAATATTGAT
    ACAGATATGGCAGCAGAAGCTATTGTAACGTCAGCATTTGGTTTTTCTGGTCAAAAATGT
    TCAGCATGTTCACGTGCAATTGTGCATAAAGACGTTTATGACGAAGTATTAGAGAAATCA
    ATCAAATTAACAAAAGAATTAACGTTGGGCAATACTGTAGACAATACGTATATGGGCCCA
    GTTATTAATAAAAAACAATTTGATAAGATTAAAAATTATATTGAGATTGGTAAAGAAGAA
    GGTAAATTAGAACAAGGTGGCGGCACTGATGATTCAAAAGGTTACTTTGTAGAACCAACA
    ATCATTTCAGGACTTAAATCTAAAGATAGAATCATGCAAGAAGAAATTTTTGGTCCAGTT
    GTTGGCTTTGTGAAAGTTAACGATTTTGATGAAGCAATTGAGGTTGCAAATGATACAGAT
    TACGGTTTAACTGGTGCAGTGATTACAAATAATCGCGAGCATTGGATTAAAGCGGTAAAC
    GAATTTGATGTAGGCAATTTATACCTTAACAGAGGCTGTACTTCAGCAGTTGTTGGATAC
    CATCCATTTGGTGGTTTCAAAATGTCAGGTACGGACGCTAAAACAGGTAGTCCAGACTAC
    TTATTACACTTCTTAGAACAAAAGGTTGTTTCAGAAATGTTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1545

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2491 [new locus tag: SAUSA300_RS13840 ]
  • symbol: SAUSA300_2491
  • description: 1-pyrroline-5-carboxylate dehydrogenase
  • length: 514
  • theoretical pI: 4.723
  • theoretical MW: 56867.1
  • GRAVY: -0.290467

⊟Function[edit | edit source]

  • reaction:
    EC 1.2.1.88?  ExPASy
    L-glutamate gamma-semialdehyde dehydrogenase L-glutamate 5-semialdehyde + NAD+ + H2O = L-glutamate + NADH
    EC 1.5.1.12?  ExPASy
    Transferred entry: 1.2.1.88
  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines putative delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01237; EC 1.2.1.88; HMM-score: 747.3)
    and 14 more
    Metabolism Energy metabolism Amino acids and amines 1-pyrroline-5-carboxylate dehydrogenase (TIGR01236; EC 1.2.1.88; HMM-score: 403)
    Cellular processes Cellular processes Adaptations to atypical conditions betaine-aldehyde dehydrogenase (TIGR01804; EC 1.2.1.8; HMM-score: 338)
    5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (TIGR02299; EC 1.2.1.60; HMM-score: 327.6)
    Metabolism Energy metabolism Amino acids and amines delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01238; EC 1.2.1.88; HMM-score: 308.5)
    Metabolism Central intermediary metabolism Other succinate-semialdehyde dehydrogenase (TIGR01780; EC 1.2.1.-; HMM-score: 306.8)
    Unknown function Enzymes of unknown specificity aldehyde dehydrogenase, Rv0768 family (TIGR04284; EC 1.2.1.-; HMM-score: 262.2)
    Metabolism Energy metabolism Other 2-hydroxymuconic semialdehyde dehydrogenase (TIGR03216; EC 1.2.1.-; HMM-score: 258.1)
    Metabolism Energy metabolism Amino acids and amines methylmalonate-semialdehyde dehydrogenase (acylating) (TIGR01722; EC 1.2.1.27; HMM-score: 254.5)
    1-pyrroline dehydrogenase (TIGR03374; EC 1.2.1.19; HMM-score: 241.4)
    putative phosphonoacetaldehyde dehydrogenase (TIGR03250; EC 1.2.1.-; HMM-score: 241.2)
    Metabolism Energy metabolism Amino acids and amines succinylglutamate-semialdehyde dehydrogenase (TIGR03240; EC 1.2.1.71; HMM-score: 239.5)
    phenylacetic acid degradation protein paaN (TIGR02278; HMM-score: 121.6)
    phenylacetic acid degradation protein paaN (TIGR02288; HMM-score: 51.6)
    Metabolism Amino acid biosynthesis Glutamate family glutamate-5-semialdehyde dehydrogenase (TIGR00407; EC 1.2.1.41; HMM-score: 20.5)
  • TheSEED  :
    • L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88)
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine and Ornithine Degradation  Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)
    and 1 more
    Amino Acids and Derivatives Proline and 4-hydroxyproline Proline, 4-hydroxyproline uptake and utilization  Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)
  • PFAM:
    ALDH-like (CL0099) Aldedh; Aldehyde dehydrogenase family (PF00171; HMM-score: 520.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002866
    • TAT(Tat/SPI): 0.000603
    • LIPO(Sec/SPII): 0.000552
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGEKIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELMLRVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDREGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLMEILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEGQNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYDEVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDSKGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITNNREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQKVVSEMF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAUSA300_1080(ftsZ)cell division protein FtsZ  [1] (data from MRSA252)
    SAUSA300_1362(hup)DNA-binding protein HU  [1] (data from MRSA252)
    SAUSA300_0996(lpdA)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    SAUSA300_0993(pdhA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    SAUSA300_0994(pdhB)pyruvate dehydrogenase E1 component, beta subunit  [1] (data from MRSA252)
    SAUSA300_0908(ppnK)inorganic polyphosphate/ATP-NAD kinase  [1] (data from MRSA252)
    SAUSA300_1644(pyk)pyruvate kinase  [1] (data from MRSA252)
    SAUSA300_0523(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    SAUSA300_2201(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    SAUSA300_2204(rplC)50S ribosomal protein L3  [1] (data from MRSA252)
    SAUSA300_2185(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    SAUSA300_1134(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    SAUSA300_1603(rplU)50S ribosomal protein L21  [1] (data from MRSA252)
    SAUSA300_2199(rplV)50S ribosomal protein L22  [1] (data from MRSA252)
    SAUSA300_1149(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    SAUSA300_2198(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    SAUSA300_2187(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SAUSA300_1306(sucA)2-oxoglutarate dehydrogenase E1 component  [1] (data from MRSA252)
    SAUSA300_1305(sucB)dihydrolipoamide succinyltransferase  [1] (data from MRSA252)
    SAUSA300_0533(tuf)elongation factor Tu  [1] (data from MRSA252)
    SAUSA300_0658LysR family transcriptional regulator  [1] (data from MRSA252)
    SAUSA300_0844hypothetical protein  [1] (data from MRSA252)
    SAUSA300_0995branched-chain alpha-keto acid dehydrogenase subunit E2  [1] (data from MRSA252)
    SAUSA300_1656universal stress protein  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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