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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1645 [new locus tag: SAUSA300_RS08980 ]
  • pan locus tag?: SAUPAN004325000
  • symbol: pfkA
  • pan gene symbol?: pfkA
  • synonym:
  • product: 6-phosphofructokinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1645 [new locus tag: SAUSA300_RS08980 ]
  • symbol: pfkA
  • product: 6-phosphofructokinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1806542..1807465
  • length: 924
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAATGCTGCCGTAAGAGCAGTTGTTCGTACAGCAATTTACAATGAAATTGAAGTTTAT
    GGTGTGTATCATGGTTACCAAGGATTGTTAAATGATGATATTCATAAACTTGAATTAGGA
    TCAGTTGGGGATACGATTCAGCGTGGAGGTACATTCTTGTATTCAGCAAGATGTCCAGAG
    TTTAAGGAGCAAGAAGTACGTAAAGTTGCAATCGAAAACTTACGTAAAAGAGGGATTGAG
    GGCCTTGTAGTTATTGGTGGTGACGGTAGTTATCGCGGTGCACAACGCATCAGTGAGGAA
    TGTAAAGAAATTCAAACTATCGGTATTCCTGGTACGATTGACAATGATATCAATGGTACT
    GATTTTACAATTGGATTTGACACAGCATTAAATACGATTATTGGCTTAGTCGACAAAATT
    AGAGATACTGCGTCAAGTCACGCACGAACATTTATCATTGAAGCAATGGGCCGTGATTGT
    GGAGATCTAGCATTATGGGCTGGATTATCAGTTGGTGCTGAGACAATTGTAGTTCCAGAA
    GTGAAAACAGATATTAAAGAAATAGCTGATAAAATTGAACAAGGTATTAAACGTGGTAAG
    AAACACTCAATCGTTCTTGTAGCAGAAGGTTGTATGACTGCGCAAGATTGTCAAAAAGAA
    TTATCACAATACATCAATGTTGATAATAGAGTGTCTGTGTTAGGTCACGTTCAACGTGGT
    GGTAGCCCAACAGGTGCGGATAGAGTTTTAGCATCACGTTTAGGTGGATATGCGGTAGAC
    TTATTAATGCAAGGTGAAACAGCTAAGGGTGTTGGAATTAAGAACAATAAAATTGTAGCA
    ACATCTTTTGATGAAATTTTTGATGGTAAAGATCATAAATTTGATTATAGTCTATATGAA
    CTTGCTAACAAGTTATCTATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    924

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1645 [new locus tag: SAUSA300_RS08980 ]
  • symbol: PfkA
  • description: 6-phosphofructokinase
  • length: 307
  • theoretical pI: 5.45417
  • theoretical MW: 33396.7
  • GRAVY: -0.167752

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.11?  ExPASy
    6-phosphofructokinase ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate
  • TIGRFAM:
    Metabolism Energy metabolism Glycolysis/gluconeogenesis 6-phosphofructokinase (TIGR02482; EC 2.7.1.11; HMM-score: 396.5)
    and 3 more
    Metabolism Energy metabolism Glycolysis/gluconeogenesis phosphofructokinase (TIGR02483; HMM-score: 273.9)
    6-phosphofructokinase (TIGR02478; EC 2.7.1.11; HMM-score: 263.9)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis diphosphate--fructose-6-phosphate 1-phosphotransferase (TIGR02477; EC 2.7.1.90; HMM-score: 76.9)
  • TheSEED  :
    • 6-phosphofructokinase (EC 2.7.1.11)
    Carbohydrates Central carbohydrate metabolism Glycolysis and Gluconeogenesis  6-phosphofructokinase (EC 2.7.1.11)
    and 1 more
    Carbohydrates Monosaccharides D-Tagatose and Galactitol Utilization  6-phosphofructokinase (EC 2.7.1.11)
  • PFAM:
    PFK (CL0240) PFK; Phosphofructokinase (PF00365; HMM-score: 338.6)
    and 2 more
    NAD_kinase; ATP-NAD kinase (PF01513; HMM-score: 13)
    DAGK_cat; Diacylglycerol kinase catalytic domain (PF00781; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005914
    • TAT(Tat/SPI): 0.000308
    • LIPO(Sec/SPII): 0.000543
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNAAVRAVVRTAIYNEIEVYGVYHGYQGLLNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGGDGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTASSHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHSIVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDLLMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAUSA300_2142(asp23)alkaline shock protein 23  [1] (data from MRSA252)
    SAUSA300_0532(fusA)elongation factor G  [1] (data from MRSA252)
    SAUSA300_0996(lpdA)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    SAUSA300_2541(mqo)malate:quinone oxidoreductase  [1] (data from MRSA252)
    SAUSA300_0993(pdhA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    SAUSA300_0994(pdhB)pyruvate dehydrogenase E1 component, beta subunit  [1] (data from MRSA252)
    SAUSA300_1644(pyk)pyruvate kinase  [1] (data from MRSA252)
    SAUSA300_0523(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    SAUSA300_2201(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    SAUSA300_2197(rplP)50S ribosomal protein L16  [1] (data from MRSA252)
    SAUSA300_1134(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    SAUSA300_2187(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SAUSA300_1305(sucB)dihydrolipoamide succinyltransferase  [1] (data from MRSA252)
    SAUSA300_0533(tuf)elongation factor Tu  [1] (data from MRSA252)
    SAUSA300_0844hypothetical protein  [1] (data from MRSA252)
    SAUSA300_0995branched-chain alpha-keto acid dehydrogenase subunit E2  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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