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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1616 [new locus tag: NWMN_RS09065 ]
  • pan locus tag?: SAUPAN004378000
  • symbol: NWMN_1616
  • pan gene symbol?: —
  • synonym:
  • product: aminotransferase, class V

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1616 [new locus tag: NWMN_RS09065 ]
  • symbol: NWMN_1616
  • product: aminotransferase, class V
  • replicon: chromosome
  • strand: +
  • coordinates: 1793578..1794738
  • length: 1161
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCTGATGCCATT
    ATGAGAGAAATTCAAGCACCTATGGTTGGTCATCGTTCTAAAGATTTTGAAGACATCGCA
    CAACAAGCATTTCAAGGTCTAAAGCCAATATTTGGGAGTCAAAATGATGTACTTATTTTA
    ACATCTAGCGGTACAAGCGTCTTGGAGGCTAGTATGTTGAACATTGTAAACCCTGAAGAT
    CACTTCGTTGTCATTGTTTCAGGTGCCTTTGGTAACCGATTTAAACAAATTGCACAAACT
    TATTACAAAAATGTGCATATTTATGACGTAACATGGGGAGAAGCTGTAGATGTCAAAGAT
    TTCATCAATTTCCTTTCAACTTTAAATGTTGAAGTTAAAGCAGTATTTAGTCAATATTGC
    GAAACATCTACGACAGTGCTACACCCTATTCACGAGTTAGGAAATGCCATTAATCAATTT
    AATAGTAATATTTATTTTGTAGTTGACGGCGTAAGTTGCATTGGTGCTGTTGATGTTGAC
    ATTAACAAAGATAAAATTGATGTACTTGTTTCTGGTAGTCAAAAAGCAATTATGTTACCT
    CCAGGATTAGCTTTTGTAGCTTATAGCCACCGTGCAAAAGAACATTTCAAAGAAGTAACT
    ACGCCAAAATTTTATCTAGACTTAAATAAATACATTTCGTCACAAGCTGACAATTCTACA
    CCGTTCACACCAAATGTGTCTTTATTTAGAGGTGTAAATGCATACGTTGAAACCGTAAAA
    GCAGAAGGTTTCAATCACGTAATAGCACGACACTATGCAATTAGAAATGCATTAAGAAGC
    GCCTTAAAAGCATTAGATTTAACTTTATTAGTCAATGATAAAGATGCATCTCCAACGGTT
    ACAGCATTCAAACCTAATACAAATGATGAAGTGAAAATAATCAAAGATGAACTTAAAAAT
    CGCTTTAAAATAACAATTGCTGGTGGTCAAGGCCATCTTAAAGGTCAAATTTTAAGAATT
    GGTCATATGGGGAAAATTAGTCCTTTCGATATTTTATCGGTAGTATCTGCTTTAGAAATT
    ATTTTAACTGAACACCGTAAAGTTAACTATATCGGTAAAGGTATATCAAAATATATGGAG
    GTTATTCATGAAGCAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1161

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1616 [new locus tag: NWMN_RS09065 ]
  • symbol: NWMN_1616
  • description: aminotransferase, class V
  • length: 386
  • theoretical pI: 7.24118
  • theoretical MW: 42849.9
  • GRAVY: -0.0124352

⊟Function[edit | edit source]

  • TIGRFAM:
    2-aminoethylphosphonate aminotransferase (TIGR03301; EC 2.6.1.-; HMM-score: 171.9)
    and 10 more
    Metabolism Central intermediary metabolism Phosphorus compounds 2-aminoethylphosphonate--pyruvate transaminase (TIGR02326; EC 2.6.1.37; HMM-score: 123.9)
    Metabolism Amino acid biosynthesis Serine family phosphoserine transaminase (TIGR01364; EC 2.6.1.52; HMM-score: 37.7)
    cysteine desulfurase NifS (TIGR03402; EC 2.8.1.7; HMM-score: 32.3)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01977; HMM-score: 28)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs ergothioneine biosynthesis PLP-dependent enzyme EgtE (TIGR04343; EC 4.4.-.-; HMM-score: 25.5)
    Genetic information processing DNA metabolism Restriction/modification cysteine desulfurase DndA (TIGR03235; EC 2.8.1.7; HMM-score: 22)
    cysteine desulfurase, NifS family (TIGR03403; EC 2.8.1.7; HMM-score: 17.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, SufS family (TIGR01979; HMM-score: 16.7)
    Genetic information processing Protein synthesis tRNA and rRNA base modification cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 13.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 13.6)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 137.5)
    and 1 more
    Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.015789
    • TAT(Tat/SPI): 0.001061
    • LIPO(Sec/SPII): 0.000712
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAFQGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYYKNVHIYDVTWGEAVDVKDFINFLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQFNSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKEHFKEVTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRNALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLKGQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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