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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01832
  • pan locus tag?: SAUPAN004378000
  • symbol: SAOUHSC_01832
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01832
  • symbol: SAOUHSC_01832
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1736778..1737938
  • length: 1161
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCTGATGCCATT
    ATGAGAGAAATTCAAGCACCTATGGTTGGTCATCGTTCTAAAGATTTTGAAGACATCGCA
    CAACAAGCATTTCAAGGTCTAAAGCCAATATTTGGGAGTCAAAATGATGTACTTATTTTA
    ACATCTAGCGGTACAAGCGTCTTGGAGGCTAGTATGTTGAACATTGTAAACCCTGAAGAT
    CACTTCGTTGTCATTGTTTCAGGTGCCTTTGGTAACCGATTTAAACAAATTGCACAAACT
    TATTACAAAAATGTGCATATTTATGACGTAACATGGGGAGAAGCTGTAGATGTCAAAGAT
    TTCATCAATTTCCTTTCAACTTTAAATGTTGAAGTTAAAGCAGTATTTAGTCAATATTGC
    GAAACATCTACGACAGTGCTACACCCTATTCACGAGTTAGGAAATGCCATTAATCAATTT
    AATAGTAATATTTATTTTGTAGTTGACGGCGTAAGTTGCATTGGTGCTGTTGATGTTGAC
    ATTAACAAAGATAAAATTGATGTACTTGTTTCTGGTAGTCAAAAAGCAATTATGTTACCT
    CCAGGATTAGCTTTTGTAGCTTATAGCCACCGTGCAAAAGAACATTTCAAAGAAGTAACT
    ACGCCAAAATTTTATCTAGACTTAAATAAATACATTTCGTCACAAGCTGACAATTCTACA
    CCGTTCACACCAAATGTGTCTTTATTTAGAGGTGTAAATGCATACGTTGAAACCGTAAAA
    GCAGAAGGTTTCAATCACGTAATAGCACGACACTATGCAATTAGAAATGCATTAAGAAGC
    GCCTTAAAAGCATTAGATTTAACTTTATTAGTCAATGATAAAGATGCATCTCCAACGGTT
    ACAGCATTCAAACCTAATACAAATGATGAAGTGAAAATAATCAAAGATGAACTTAAAAAT
    CGCTTTAAAATAACAATTGCTGGTGGTCAAGGCCATCTTAAAGGTCAAATTTTAAGAATT
    GGTCATATGGGGAAAATTAGTCCTTTCGATATTTTATCGGTAGTATCTGCTTTAGAAATT
    ATTTTAACTGAACACCGTAAAGTTAACTATATCGGTAAAGGTATATCAAAATATATGGAG
    GTTATTCATGAAGCAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1161

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01832
  • symbol: SAOUHSC_01832
  • description: hypothetical protein
  • length: 386
  • theoretical pI: 7.24118
  • theoretical MW: 42849.9
  • GRAVY: -0.0124352

⊟Function[edit | edit source]

  • TIGRFAM:
    2-aminoethylphosphonate aminotransferase (TIGR03301; EC 2.6.1.-; HMM-score: 171.9)
    and 10 more
    Metabolism Central intermediary metabolism Phosphorus compounds 2-aminoethylphosphonate--pyruvate transaminase (TIGR02326; EC 2.6.1.37; HMM-score: 123.9)
    Metabolism Amino acid biosynthesis Serine family phosphoserine transaminase (TIGR01364; EC 2.6.1.52; HMM-score: 37.7)
    cysteine desulfurase NifS (TIGR03402; EC 2.8.1.7; HMM-score: 32.3)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01977; HMM-score: 28)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs ergothioneine biosynthesis PLP-dependent enzyme EgtE (TIGR04343; EC 4.4.-.-; HMM-score: 25.5)
    Genetic information processing DNA metabolism Restriction/modification cysteine desulfurase DndA (TIGR03235; EC 2.8.1.7; HMM-score: 22)
    cysteine desulfurase, NifS family (TIGR03403; EC 2.8.1.7; HMM-score: 17.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, SufS family (TIGR01979; HMM-score: 16.7)
    Genetic information processing Protein synthesis tRNA and rRNA base modification cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 13.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 13.6)
  • TheSEED  :
    • Phosphoserine aminotransferase, putative (EC 2.6.1.52)
    Amino Acids and Derivatives Alanine, serine, and glycine Serine Biosynthesis  Phosphoserine aminotransferase, putative (EC 2.6.1.52)
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 137.5)
    and 1 more
    Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.015789
    • TAT(Tat/SPI): 0.001061
    • LIPO(Sec/SPII): 0.000712
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAFQGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYYKNVHIYDVTWGEAVDVKDFINFLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQFNSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKEHFKEVTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRNALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLKGQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY* (repression) regulon
    CodY*(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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