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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1929 [new locus tag: NWMN_RS11130 ]
  • pan locus tag?: SAUPAN005193000
  • symbol: NWMN_1929
  • pan gene symbol?:
  • synonym:
  • product: succinyl-diaminopimelate desuccinylase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1929 [new locus tag: NWMN_RS11130 ]
  • symbol: NWMN_1929
  • product: succinyl-diaminopimelate desuccinylase
  • replicon: chromosome
  • strand: +
  • coordinates: 2135805..2137091
  • length: 1287
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTTTAAGAATAAAAATTATGTTTATATAAATATATATACTACTTTGAAAGGTGTGAGC
    TTAATGACAACTTTTAGTGAAAAAGAAAAAATTCAATTACTAGCAGATATTGTTGAACTA
    CAAACTGAAAATAATAATGAAATAGACGTTTGTAATTATTTAACAGATTTATTCGACAAG
    TACGATATTAAATCTGAAATTTTGAAAGTTAATGAACACCGCGCCAATATCGTTGCAGAA
    ATCGGTAACGGCTCACCTATACTCGCATTGAGTGGTCATATGGATGTTGTTGATGCAGGA
    AATCAAGATAATTGGTCATATCCCCCTTTTCAACTGACAGAAAAAGATGGCAAATTATAT
    GGCCGAGGCACTACAGATATGAAAGGCGGTTTAATGGCTTTGGTCGTATCTCTAATCGAA
    TTAAAAGAACAAAATGAATTGCCTCATGGAACGATTAGATTACTGGCTACTGCTGGCGAA
    GAGAAAGAACAAGAAGGTGCCAAATTATTAGCTGATAAAGGCTATTTAGACGATGTCGAT
    GGCTTAATTATTGCTGAACCAACTGGATCTGGAATTTATTATGCACATAAGGGGTCTATG
    TCATGTAAAGTAACTGCAACTGGTAAAGCTGTCCATAGCTCAGTTCCATTTATTGGTGAC
    AATGCAATTGATACACTGCTTGAATTTTATAATCTATTTAAAGAAAAATATTCAGAGCTT
    AAACAACAAGATACTAAACATGAATTAGATGTTGCGCCTATGTTCAAATCATTGATTGGA
    AAAGAAATTTCTGAAGAGGATGCAAATTATGCATCTGGTCTTACAGCTGTATGTTCGATT
    ATAAATGGCGGCAAACAATTTAACTCTGTACCAGATGAAGCTTCACTTGAATTTAACGTA
    AGACCAGTTCCTGAGTATGATAACGACTTTATAGAATCGTTTTTCCAAAATATCATTAAT
    GATGTGGATAGCAATAAGCTTTCACTCGATATTCCAAGCAATCACCGACCTGTAACAAGC
    GATAAAAATAGCAAATTAATTACTACGATTAAAGATGTAGCTTCTAGTTATGTAGAACAA
    GACGAAATATTTGTTTCAGCGCTTGTAGGTGCAACAGATGCCTCTAGTTTCTTAGGAGAT
    AATAAGGACAATGTTGATTTAGCCATTTTTGGACCAGGTAATCCATTAATGGCACATCAA
    ATCGATGAATATATTGAAAAAGATATGTATCTGAAATATATTGATATTTTTAAAGAGGCT
    TCCATTCAATATTTAAAAGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1287

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_1929 [new locus tag: NWMN_RS11130 ]
  • symbol: NWMN_1929
  • description: succinyl-diaminopimelate desuccinylase
  • length: 428
  • theoretical pI: 4.3647
  • theoretical MW: 47589.3
  • GRAVY: -0.311449

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 300.4)
    and 11 more
    Metabolism Amino acid biosynthesis Glutamate family acetylornithine deacetylase (ArgE) (TIGR01892; EC 3.5.1.16; HMM-score: 167.6)
    Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01246; EC 3.5.1.18; HMM-score: 134.1)
    Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01900; EC 3.5.1.18; HMM-score: 121.7)
    putative selenium metabolism hydrolase (TIGR03526; HMM-score: 114.7)
    N-acetyl-ornithine/N-acetyl-lysine deacetylase (TIGR01902; HMM-score: 100.4)
    putative dipeptidase (TIGR01887; EC 3.4.13.-; HMM-score: 95)
    N-acyl-L-amino-acid amidohydrolase (TIGR01880; EC 3.5.1.14; HMM-score: 78.2)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides amidohydrolase (TIGR01891; HMM-score: 75.3)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides dipeptidase PepV (TIGR01886; EC 3.4.13.-; HMM-score: 51.5)
    peptidase T-like protein (TIGR01883; HMM-score: 31.1)
    Xaa-His dipeptidase (TIGR01893; EC 3.4.13.20; HMM-score: 29)
  • TheSEED:  
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Biosynthesis extended  Acetylornithine deacetylase (EC 3.5.1.16)
  • PFAM:
    Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 137.3)
    and 2 more
    no clan defined M20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 81.3)
    Peptidase_MH (CL0035) Peptidase_M28; Peptidase family M28 (PF04389; HMM-score: 21.5)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.050778
    • TAT(Tat/SPI): 0.00081
    • LIPO(Sec/SPII): 0.004013
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MFKNKNYVYINIYTTLKGVSLMTTFSEKEKIQLLADIVELQTENNNEIDVCNYLTDLFDKYDIKSEILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWSYPPFQLTEKDGKLYGRGTTDMKGGLMALVVSLIELKEQNELPHGTIRLLATAGEEKEQEGAKLLADKGYLDDVDGLIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNLFKEKYSELKQQDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLEFNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVASSYVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKYIDIFKEASIQYLKEK

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

  • NWMN_1929 no polycistronic organisation predicted

Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation [1]   other strains

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

Relevant publications[edit | edit source]