PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1948 [new locus tag: NWMN_RS11240 ]
• pan locus tag^?: SAUPAN005284000
• symbol: scrB
• pan gene symbol^?: scrB
• synonym:
• product: sucrose-6-phosphate hydrolase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1948 [new locus tag: NWMN_RS11240 ]
• symbol: scrB
• product: sucrose-6-phosphate hydrolase
• replicon: chromosome
• strand: -
• coordinates: 2155602..2157086
• length: 1485
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331202 NCBI
• RefSeq: YP_001332982 NCBI
• BioCyc:
• MicrobesOnline: 3707536 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACCGAATGGACTAGAGAAGAACGTTATCAACGAATCGAGGACGTTGATACTGAGTAT
  TTTAAAACATTAAAACAACAAGTTGATCAATCAAAATTTCGTCAACAATTTCATATACAA
  CCAGAAACAGGCTTATTAAATGACCCCAACGGACTTATTTTTTATAAAGGGAAGTATTAT
  GTTTCACATCAATGGTTCCCATTAGGCGCAGTACATGGCTTAAAGTATTGGTATAACTAC
  ACGAGTGATGACTTAATAAACTTTAAAGCTGAAGGGCCAATTTTAAATCCAGATACTAAA
  TATGACAGCCATGGTGTATATAGCGGTAGCGCTTTTGAATATAACGGGCATTTATATTAT
  ATGTACACAGGAAATCATCGAGATAATCATTGGCAACGACATGCGAGTCAGATGATCGCA
  CGATTGAAAGAAGACGGTTCAGTTGAAAAGTTTCCAAAGCCAGTAATTAGCCAACAACCA
  GAAGGATATACGAGTCATTTTAGAGATCCTAAAGTTTTTAAATATGATGAGAAATATTAT
  GCAATCATTGGTGCACAAAATAATGATCAGCAAGGTCGATTATTACTTTATAATACTGAA
  GATATAATTAATTGGCATTATTTAGGTGAAATAAATACAGAGTTGGATGATTTTGGATAT
  ATGTGGGAATGCCCAGATTACTTTAATGTAGATAATCAAGATGTCATACTTATTTGTCCA
  CAAGGTATTGAACCAAAAGGCGATCAGTTCAAAAATATTTATCAAAGTGGTTATATACTT
  GGAAAGTTTGATATTGAAAAGTTAACATATGAACATGAAAATTTTGTCGAGCTTGATAAT
  GGTTTTGATTTCTATGCACCTCAAACATTTTTAGATGAAAAAGGCCGACGAGTACTAATT
  GGATGGATGGGGTTACCGGAAATCGAATATCCTACTGATAATGAAGGATGGGCCCATTGC
  TTAACAATTCCTCGTGTATTAAATGTAGAAAATGGTCAACTTAAGCAACGTCCATATCCA
  GCGTTGGAAAAATTACGTCACAATAAAGAGACAGCATTAGGCTACGCAAATAAATTTACT
  CGAAAATTACATCCGTATGAAGGTAAACAGTATGAATTAATCATAGATATTTTGGATAAT
  GATGCTACCGAAGTGTACTTTGAATTACGTACATCTAAGACTTCTTCAACATTAATTGCT
  TATAACAAGCGTGAAAATAAAATAACATTAGATCGCAGCGACAGTGGTTTATTGCCGACA
  AATGTTGAAGGTACGACGCGTAGTACGATATTAGACACGCCATTAAAGCAATTACAAATT
  TTTGTTGATACATCTAGTATAGAAATTTTCTGTAATGATGGAGAACGTGTTTTGACATCT
  CGAATTTTCCCGACAGAAGACGCACTAGGTATTAAAACATCAACAGAATCAGGGCAAGTA
  TATTTACAATTTACTAAATATGATTTAAAGGATGAACATAAATGA
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⊟Protein

⊟General

• locus tag: NWMN_1948 [new locus tag: NWMN_RS11240 ]
• symbol: ScrB
• description: sucrose-6-phosphate hydrolase
• length: 494
• theoretical pI: 5.03855
• theoretical MW: 57927.2
• GRAVY: -0.743117

⊟Function

• reaction:
  EC 3.2.1.26?  ExPASy
  Beta-fructofuranosidase Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
• TIGRFAM:
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides sucrose-6-phosphate hydrolase (TIGR01322; EC 3.2.1.-; HMM-score: 613)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  B_Fructosidase (CL0143) Glyco_hydro_32N; Glycosyl hydrolases family 32 N-terminal domain (PF00251; HMM-score: 331.7)
  and 2 more
  Concanavalin (CL0004) Glyco_hydro_32C; Glycosyl hydrolases family 32 C terminal (PF08244; HMM-score: 82.6)
  B_Fructosidase (CL0143) Glyco_hydro_130; beta-1,4-mannooligosaccharide phosphorylase (PF04041; HMM-score: 17.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007677
  • TAT(Tat/SPI): 0.001512
  • LIPO(Sec/SPII): 0.00133
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222160 NCBI
• RefSeq: YP_001332982 NCBI
• UniProt: A0A0H3K9M9 UniProt

⊟Protein sequence

• MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETGLLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYDSHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQPEGYTSHFRDPKVFKYDEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDFGYMWECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFVELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQLKQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTSKTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIFCNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1947 < scrB

⊟Regulation

• regulator: ScrR (repression) regulon

  ScrR

  (TF)

  important in Sucrose utilization; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications