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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02268
  • pan locus tag?: SAUPAN005284000
  • symbol: SAOUHSC_02268
  • pan gene symbol?: scrB
  • synonym:
  • product: sucrose-6-phosphate hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02268
  • symbol: SAOUHSC_02268
  • product: sucrose-6-phosphate hydrolase
  • replicon: chromosome
  • strand: -
  • coordinates: 2097955..2099439
  • length: 1485
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACCGAATGGACTAGAGAAGAACGTTATCAACGAATCGAGGACGTTGATACTGAGTAT
    TTTAAAACATTAAAACAACAAGTTGATCAATCAAAATTTCGTCAACAATTTCATATACAA
    CCAGAAACAGGCTTATTAAATGACCCCAACGGACTTATTTTTTATAAAGGGAAGTATTAT
    GTTTCACATCAATGGTTCCCATTAGGCGCAGTACATGGCTTAAAGTATTGGTATAACTAC
    ACGAGTGATGACTTAATAAACTTTAAAGCTGAAGGGCCAATTTTAAATCCAGATACTAAA
    TATGACAGCCATGGTGTATATAGCGGTAGCGCTTTTGAATATAACGGGCATTTATATTAT
    ATGTACACAGGAAATCATCGAGATAATCATTGGCAACGACATGCGAGTCAGATGATCGCA
    CGATTGAAAGAAGACGGTTCAGTTGAAAAGTTTCCAAAGCCAGTAATTAGCCAACAACCA
    GAAGGATATACGAGTCATTTTAGAGATCCTAAAGTTTTTAAATATGATGAGAAATATTAT
    GCAATCATTGGTGCACAAAATAATGATCAGCAAGGTCGATTATTACTTTATAATACTGAA
    GATATAATTAATTGGCATTATTTAGGTGAAATAAATACAGAGTTGGATGATTTTGGATAT
    ATGTGGGAATGCCCAGATTACTTTAATGTAGATAATCAAGATGTCATACTTATTTGTCCA
    CAAGGTATTGAACCAAAAGGCGATCAGTTCAAAAATATTTATCAAAGTGGTTATATACTT
    GGAAAGTTTGATATTGAAAAGTTAACATATGAACATGAAAATTTTGTCGAGCTTGATAAT
    GGTTTTGATTTCTATGCACCTCAAACATTTTTAGATGAAAAAGGCCGACGAGTACTAATT
    GGATGGATGGGGTTACCGGAAATCGAATATCCTACTGATAATGAAGGATGGGCCCATTGC
    TTAACAATTCCTCGTGTATTAAATGTAGAAAATGGTCAACTTAAGCAACGTCCATATCCA
    GCGTTGGAAAAATTACGTCACAATAAAGAGACAGCATTAGGCTACGCAAATAAATTTACT
    CGAAAATTACATCCGTATGAAGGTAAACAGTATGAATTAATCATAGATATTTTGGATAAT
    GATGCTACCGAAGTGTACTTTGAATTACGTACATCTAAGACTTCTTCAACATTAATTGCT
    TATAACAAGCGTGAAAATAAAATAACATTAGATCGCAGCGACAGTGGTTTATTGCCGACA
    AATGTTGAAGGTACGACGCGTAGTACGATATTAGACACGCCATTAAAGCAATTACAAATT
    TTTGTTGATACATCTAGTATAGAAATTTTCTGTAATGATGGAGAACGTGTTTTGACATCT
    CGAATTTTCCCGACAGAAGACGCACTAGGTATTAAAACATCAACAGAATCAGGGCAAGTA
    TATTTACAATTTACTAAATATGATTTAAAGGATGAACATAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1485

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02268
  • symbol: SAOUHSC_02268
  • description: sucrose-6-phosphate hydrolase
  • length: 494
  • theoretical pI: 5.03855
  • theoretical MW: 57927.2
  • GRAVY: -0.743117

⊟Function[edit | edit source]

  • reaction:
    EC 3.2.1.26?  ExPASy
    Beta-fructofuranosidase Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
  • TIGRFAM:
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides sucrose-6-phosphate hydrolase (TIGR01322; EC 3.2.1.-; HMM-score: 613)
  • TheSEED  :
    • Sucrose-6-phosphate hydrolase (EC 3.2.1.B3)
    Carbohydrates Di- and oligosaccharides Sucrose utilization  Sucrose-6-phosphate hydrolase (EC 3.2.1.B3)
  • PFAM:
    B_Fructosidase (CL0143) Glyco_hydro_32N; Glycosyl hydrolases family 32 N-terminal domain (PF00251; HMM-score: 331.7)
    and 2 more
    Concanavalin (CL0004) Glyco_hydro_32C; Glycosyl hydrolases family 32 C terminal (PF08244; HMM-score: 82.6)
    B_Fructosidase (CL0143) Glyco_hydro_130; beta-1,4-mannooligosaccharide phosphorylase (PF04041; HMM-score: 17.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007677
    • TAT(Tat/SPI): 0.001512
    • LIPO(Sec/SPII): 0.00133
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETGLLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYDSHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQPEGYTSHFRDPKVFKYDEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDFGYMWECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFVELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQLKQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTSKTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIFCNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: ScrR* (repression) regulon
    ScrR*(TF)important in Sucrose utilization; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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