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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02269
  • pan locus tag?: SAUPAN005285000
  • symbol: SAOUHSC_02269
  • pan gene symbol?: scrR
  • synonym:
  • product: sucrose operon repressor

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02269
  • symbol: SAOUHSC_02269
  • product: sucrose operon repressor
  • replicon: chromosome
  • strand: -
  • coordinates: 2099588..2100538
  • length: 951
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAAATATATCTGATATTGCCAAATTGGCAGGCGTTTCAAAAAGTACAGTATCTAGA
    TTTTTAAATAATGGATCTGTCAGTAAAAAAACAAGTGAAAAATTAACAAGAATTATAGCA
    GAACATGACTATCAACCGAATCAATTTGCTCAAAGTTTAAGAGCGAGACAAACACACCTT
    ATTGGTGCCATTATACCTAGAATGAATTCATATGCAGTAGATGAAACAATCAAAGGATTG
    GCAAAACAATGCCAAAAATATGAATCACAATTAATTTTAAATTACACAGGTTTAAATATC
    GAAGCAGAAATACAAGCGCTTGAAACATTAGCACGCAGTAAAGTAGATGGTATTGTTTTA
    ATGGCTACAGACATAACAGAGAGACATATTGAAGTCATTAATAAAATGAATGTACCAATC
    GTTATTGTTGGTCAACAACATGAACAACTTCATAGTATTGTGCATGATGATTATAAAGCA
    GGTCAAATTATAGGCGAATGGATTGGTCAACAGGGATATCAACAAGTTGAAGTGTTTAGT
    GTAAGTGAAAAAGATATTGCAGTTGGTATACATAGAAAACGTGGTTTACTTGACCAGTTA
    GCTAAATACCAAATTAAACCTAATATACATGAAACAAATTTTACTTATGTGGAAGCACAA
    AAAGATGTTGCAAATGTTTTGGAAAATGTGGAGCAAGTAGATGCGGTTGTTGGAGCAACT
    GATACGATTGCATTAGCTGCCTATAAATATTATTCTGATAAAAAAGATGTTATGAAACCA
    CATCAAATATATGGTTTTGGTGGTGACCCAATGACACAATTAGTGTCTCCATCGATAAAA
    ACAATTCATTATAATTATTTTGAAGCTGGCCAATGCGCGATGGAAGAGATACAACAGATG
    CTTAAAAAGCAAGATATGCCATATAGCGTCACAGTAGATGTTAATATTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    951

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02269
  • symbol: SAOUHSC_02269
  • description: sucrose operon repressor
  • length: 316
  • theoretical pI: 6.6818
  • theoretical MW: 35499.3
  • GRAVY: -0.297785

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02405; HMM-score: 150.5)
    and 3 more
    Signal transduction Regulatory functions DNA interactions catabolite control protein A (TIGR01481; HMM-score: 119.5)
    Signal transduction Regulatory functions DNA interactions D-fructose-responsive transcription factor (TIGR02417; HMM-score: 114.2)
    CRISPR-associated endonuclease Cas1 (TIGR00287; EC 3.1.-.-; HMM-score: 15.9)
  • TheSEED  :
    • Sucrose operon repressor ScrR, LacI family
    Carbohydrates Di- and oligosaccharides Sucrose utilization  Sucrose operon repressor ScrR, LacI family
  • PFAM:
    HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 64.3)
    Periplas_BP (CL0144) Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 63.1)
    and 22 more
    Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 46.7)
    Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 38.9)
    HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 20.8)
    HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 16.8)
    HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 16.7)
    no clan defined Vasohibin; Vasohibin (PF14822; HMM-score: 15.4)
    Cas_Cas1; CRISPR associated protein Cas1 (PF01867; HMM-score: 15.1)
    HTH (CL0123) HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 14.6)
    HTH_6; Helix-turn-helix domain, rpiR family (PF01418; HMM-score: 14.5)
    DUF3853; Protein of unknown function (DUF3853) (PF12964; HMM-score: 14.3)
    HTH_17; Helix-turn-helix domain (PF12728; HMM-score: 14)
    HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 13.9)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 13.7)
    HTH_10; HTH DNA binding domain (PF04967; HMM-score: 13.3)
    TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 12.9)
    6PGD_C (CL0106) NAD_binding_11; NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase (PF14833; HMM-score: 12.9)
    HTH (CL0123) HTH_29; Winged helix-turn helix (PF13551; HMM-score: 12.8)
    HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 12.7)
    MarR; MarR family (PF01047; HMM-score: 12.4)
    HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 12.4)
    MarR_2; MarR family (PF12802; HMM-score: 12)
    HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effector: Sucrose-6-phosphate
  • genes regulated by ScrR*, TF important in Sucrose utilizationRegPrecise
    repression
    fruC* < scrB*
    transcription unit transferred from N315 data RegPrecise

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010468
    • TAT(Tat/SPI): 0.001683
    • LIPO(Sec/SPII): 0.001749
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDYQPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEAEIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKAGQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVEAQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVSPSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA* regulon
    CcpA*(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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