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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2030 [new locus tag: SACOL_RS10600 ]
  • pan locus tag?: SAUPAN005285000
  • symbol: scrR
  • pan gene symbol?: scrR
  • synonym:
  • product: sucrose operon repressor

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2030 [new locus tag: SACOL_RS10600 ]
  • symbol: scrR
  • product: sucrose operon repressor
  • replicon: chromosome
  • strand: -
  • coordinates: 2088852..2089802
  • length: 951
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAAATATATCTGATATTGCCAAATTGGCAGGCGTTTCAAAAAGTACAGTATCTAGA
    TTTTTAAATAATGGATCTGTCAGTAAAAAAACAAGTGAAAAATTAACAAGAATTATAGCA
    GAACATGACTATCAACCGAATCAATTTGCTCAAAGTTTAAGAGCGAGACAAACACACCTT
    ATTGGTGCCATTATACCTAGAATGAATTCATATGCAGTAGATGAAACAATCAAAGGATTG
    GCAAAACAATGCCAAAAATATGAATCACAATTAATTTTAAATTACACAGGTTTAAATATC
    GAAGCAGAAATACAAGCGCTTGAAACATTAGCACGCAGTAAAGTAGATGGTATTGTTTTA
    ATGGCTACAGACATAACAGAGAGACATATTGAAGTCATTAATAAAATGAATGTACCAATC
    GTTATTGTTGGTCAACAACATGAACAACTTCATAGTATTGTGCATGATGATTATAAAGCA
    GGTCAAATTATAGGCGAATGGATTGGTCAACAGGGATATCAACAAGTTGAAGTGTTTAGT
    GTAAGTGAAAAAGATATTGCAGTTGGTATACATAGAAAACGTGGTTTACTTGACCAGTTA
    GCTAAATACCAAATTAAACCTAATATACATGAAACAAATTTTACTTATGTGGAAGCACAA
    AAAGATGTTGCAAATGTTTTGGAAAATGTGGAGCAAGTAGATGCGGTTGTTGGAGCAACT
    GATACGATTGCATTAGCTGCCTATAAATATTATTCTGATAAAAAAGATGTTATGAAACCA
    CATCAAATATATGGTTTTGGTGGTGACCCAATGACACAATTAGTGTCTCCATCGATAAAA
    ACAATTCATTATAATTATTTTGAAGCTGGCCAATGCGCGATGGAAGAGATACAACAGATG
    CTTAAAAAGCAAGATATGCCATATAGCGTCACAGTAGATGTTAATATTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    951

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2030 [new locus tag: SACOL_RS10600 ]
  • symbol: ScrR
  • description: sucrose operon repressor
  • length: 316
  • theoretical pI: 6.6818
  • theoretical MW: 35499.3
  • GRAVY: -0.297785

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02405; HMM-score: 150.5)
    and 3 more
    Signal transduction Regulatory functions DNA interactions catabolite control protein A (TIGR01481; HMM-score: 119.5)
    Signal transduction Regulatory functions DNA interactions D-fructose-responsive transcription factor (TIGR02417; HMM-score: 114.2)
    CRISPR-associated endonuclease Cas1 (TIGR00287; EC 3.1.-.-; HMM-score: 15.9)
  • TheSEED  :
    • Sucrose operon repressor ScrR, LacI family
    Carbohydrates Di- and oligosaccharides Sucrose utilization  Sucrose operon repressor ScrR, LacI family
  • PFAM:
    HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 64.3)
    Periplas_BP (CL0144) Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 63.1)
    and 22 more
    Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 46.7)
    Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 38.9)
    HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 20.8)
    HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 16.8)
    HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 16.7)
    no clan defined Vasohibin; Vasohibin (PF14822; HMM-score: 15.4)
    Cas_Cas1; CRISPR associated protein Cas1 (PF01867; HMM-score: 15.1)
    HTH (CL0123) HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 14.6)
    HTH_6; Helix-turn-helix domain, rpiR family (PF01418; HMM-score: 14.5)
    DUF3853; Protein of unknown function (DUF3853) (PF12964; HMM-score: 14.3)
    HTH_17; Helix-turn-helix domain (PF12728; HMM-score: 14)
    HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 13.9)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 13.7)
    HTH_10; HTH DNA binding domain (PF04967; HMM-score: 13.3)
    TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 12.9)
    6PGD_C (CL0106) NAD_binding_11; NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase (PF14833; HMM-score: 12.9)
    HTH (CL0123) HTH_29; Winged helix-turn helix (PF13551; HMM-score: 12.8)
    HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 12.7)
    MarR; MarR family (PF01047; HMM-score: 12.4)
    HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 12.4)
    MarR_2; MarR family (PF12802; HMM-score: 12)
    HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effector: Sucrose-6-phosphate
  • genes regulated by ScrR, TF important in Sucrose utilizationRegPrecise
    repression
    fruC* < cscA
    scrA*
    transcription units transferred from N315 data RegPrecise

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010468
    • TAT(Tat/SPI): 0.001683
    • LIPO(Sec/SPII): 0.001749
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDYQPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEAEIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKAGQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVEAQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVSPSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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