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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1846 [new locus tag: SA_RS10600 ]
  • pan locus tag?: SAUPAN005284000
  • symbol: scrB
  • pan gene symbol?: scrB
  • synonym:
  • product: sucrose-6-phosphate hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1846 [new locus tag: SA_RS10600 ]
  • symbol: scrB
  • product: sucrose-6-phosphate hydrolase
  • replicon: chromosome
  • strand: -
  • coordinates: 2083528..2085012
  • length: 1485
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACCGAATGGACTAGAGAAGAACGTTATCAACGAATCGAGGACGTTGATACTGAGTAT
    TTTAAAACATTAAAACAACAAGTTGATCAATCAAAATTTCGTCAACAATTTCATATACAA
    CCAGAAACAGGTTTATTAAATGATCCCAACGGACTTATTTTTTATAAAGGGAAGTATTAT
    GTTTCACATCAATGGTTCCCATTAGGCGCAGTACATGGCTTAAAGTATTGGTATAACTAC
    ACGAGTGATGACTTAATAAACTTTAAACCTGAAGGGCCAATATTAAATCCAGATACTAAA
    TATGACAGCCATGGTGTTTATAGCGGTAGCGCTTTTGAATATAACGGGCATTTATATTAT
    ATGTACACAGGAAATCATCGAGATAATCATTGGCAACGACATGCGAGTCAGATGATCGCA
    CGATTGAAAGAAGACGGTTCAGTTGAAAAGTTTCCAAAGCCAGTAATTAGCCAGCAACCA
    GAAGGATATACAAGTCATTTTAGAGATCCTAAAGTTTTTAAATATGGTGAGAAATATTAT
    GCAATCATTGGCGCACAAAATAATGATCAGCAAGGTCGATTATTACTTTATAATACTGAA
    GATATAATTAATTGGCATTATTTAGGTGAAATAAATACAGAGTTAGATGATTTTGGATAT
    ATGTGGGAATGCCCAGATTACTTTAATTTAGATAATCAAGATGTCATACTTATTTGTCCA
    CAAGGTATTGAACCAAAAGGCAATCAGTTCAAAAATATTTATCAAAGTGGTTATATACTT
    GGAAAGTTTGATATTGAAAAGTTAACATATGAACATGAAAATTTTGTCGAGCTTGATAAT
    GGCTTTGATTTCTATGCACCTCAAACATTTTTAGATGAAAAAGGCCGACGAGTACTAATT
    GGATGGATGGGGTTACCGGAAATCGAATATCCTACTGATAATGAAGGATGGGCCCATTGC
    TTAACAATTCCTCGTGTATTAAATGTAGAAAATGGTCAACTTAAGCAACGTCCATATCCA
    GCGTTGGAAAAATTACGTCACAATAAAGAGACAGCATTAGGCTACGCAAATAAATTTACT
    CGAAAATTACATCCGTATGAAGGTAAACAGTATGAATTAATCATAGATATTTTGGATAAT
    GATGCTACCGAAGTGTACTTTGAATTACGTACATCTAAGACTTCTTCAACATTAATTGCT
    TATAACAAGCGTGAAAATAAAATAACATTAGATCGCAGCGACAGTGGTTTATTGCCGACA
    AATGTTGAAGGTACGACGCGTAGTACGATATTAGACACGCCATTAAAGCAATTACAAATT
    TTTGTTGATACATCTAGTATAGAAATTTTCTGTAATGATGGGGAACGTGTTTTGACATCT
    CGAATTTTCCCGACAGAAGACGCACTAGGTATTAAAACATCAACAGAATCAGGGCAAGTA
    TATTTACAATTTACTAAATATGATTTAAAGGATGAACATAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1485

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1846 [new locus tag: SA_RS10600 ]
  • symbol: ScrB
  • description: sucrose-6-phosphate hydrolase
  • length: 494
  • theoretical pI: 5.18236
  • theoretical MW: 57908.3
  • GRAVY: -0.744534

⊟Function[edit | edit source]

  • reaction:
    EC 3.2.1.26?  ExPASy
    Beta-fructofuranosidase Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
  • TIGRFAM:
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides sucrose-6-phosphate hydrolase (TIGR01322; EC 3.2.1.-; HMM-score: 611)
  • TheSEED  :
    • beta-fructofuranosidase (EC 3.2.1.26)
    • beta-fructofuranosidase for sucrose-6-phoshate (EC 3.2.1.26)
    Carbohydrates Di- and oligosaccharides Sucrose utilization  Sucrose-6-phosphate hydrolase (EC 3.2.1.B3)
  • PFAM:
    B_Fructosidase (CL0143) Glyco_hydro_32N; Glycosyl hydrolases family 32 N-terminal domain (PF00251; HMM-score: 328.9)
    and 2 more
    Concanavalin (CL0004) Glyco_hydro_32C; Glycosyl hydrolases family 32 C terminal (PF08244; HMM-score: 82.5)
    B_Fructosidase (CL0143) Glyco_hydro_130; beta-1,4-mannooligosaccharide phosphorylase (PF04041; HMM-score: 15.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007677
    • TAT(Tat/SPI): 0.001512
    • LIPO(Sec/SPII): 0.00133
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETGLLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKPEGPILNPDTKYDSHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQPEGYTSHFRDPKVFKYGEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDFGYMWECPDYFNLDNQDVILICPQGIEPKGNQFKNIYQSGYILGKFDIEKLTYEHENFVELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQLKQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTSKTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIFCNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: ScrR (repression) regulon
    ScrR(TF)important in Sucrose utilization; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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