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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1845 [new locus tag: SA_RS10595 ]
  • pan locus tag?: SAUPAN005283000
  • symbol: SA1845
  • pan gene symbol?: fruC
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA1845 [new locus tag: SA_RS10595 ]
  • symbol: SA1845
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2082572..2083531
  • length: 960
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGACGTCTATTTTCAATAGGAGAAGCGTTAATTGATTTTATTCCAAATGTTACAAAT
    GCGAATTTAAAAGATGTTCAAACATTTACGAAGCAAATTGGTGGCGCGCCATGCAATGTA
    GCTTGTACAGTTCAAAAGTTAGGACAACAAGCGTATATGATTACACAATTAGGTAATGAT
    GCATTTGGAGATAGCATTATTGAAACGATATCAAGTATTGGTGTAGATGTATCAAAAGTG
    TATCGCACGAATGAAGCAAACACTGCGTTAGCTTTTGTGAGTTTGACTGAAGCGGGCGAG
    CGAGATTTTTCTTTTTATCGTAAGCCCTCTGCTGATATGTTATTTGAACCAAGTTTTGTG
    AATGATATTGATGTGAATGAAAATGATGTAGTACATTTTTGTTCTGTTGATTTGGTCGAT
    AGTCCGATGAGAGATGCACATTATCAATTGATTACCAAAACATTAAATGCAAATGGTACT
    GTCGTATTTGATCCTAATGTAAGATTGCCATTATGGGATAACGCTGAAGATTTAAGACAA
    ACTATTCATACCTTTTTGCCATTGGCGCATATTGTAAAAGTTTCAGATGAAGAATTAGAA
    TTTATTACAGGTATTCATGATGAAAATGAAGCCATTCAATCATTATTTACAGGTAATGTT
    ACTGTAGTCATTTATACGAAGGGAGCAGATGGTGCTGCCGTATATTTGAAAAATGGTATT
    AATCATTATCATAGTGGCTATAAGGTAAAACCTGTTGATACAACTGGGGCAGGGGATGCT
    TTTATTGGTGCAGTTATTAGCAGGATTTTAGCAACCGATGTGTTAAATTTAACTCAGTTA
    TTTGAAAATGAAGGTGAGGAAATTTTAGCGTTTAGCAATCGCGTTGCTGCAATAGTGACA
    ACGAAGTATGGTGCCATTAATAGTTTGCCAACATTACAAGAGGTTGAACAAGCATTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA1845 [new locus tag: SA_RS10595 ]
  • symbol: SA1845
  • description: hypothetical protein
  • length: 319
  • theoretical pI: 4.38514
  • theoretical MW: 34991.2
  • GRAVY: 0.0134796

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Sugars 5-dehydro-2-deoxygluconokinase (TIGR04382; EC 2.7.1.92; HMM-score: 196.3)
    and 4 more
    Metabolism Energy metabolism Sugars ribokinase (TIGR02152; EC 2.7.1.15; HMM-score: 118.8)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides bifunctional protein RfaE, domain I (TIGR02198; EC 2.7.1.-; HMM-score: 45.1)
    hexose kinase, 1-phosphofructokinase family (TIGR03168; EC 2.7.1.-; HMM-score: 41.5)
    1-phosphofructokinase (TIGR03828; EC 2.7.1.56; HMM-score: 38.2)
  • TheSEED  :
    • Fructokinase (EC 2.7.1.4)
    Carbohydrates Di- and oligosaccharides Sucrose utilization  Fructokinase (EC 2.7.1.4)
    and 2 more
    Carbohydrates Monosaccharides Fructose utilization  Fructokinase (EC 2.7.1.4)
    Carbohydrates Sugar alcohols Mannitol Utilization  Fructokinase (EC 2.7.1.4)
  • PFAM:
    Ribokinase (CL0118) PfkB; pfkB family carbohydrate kinase (PF00294; HMM-score: 279.9)
    and 1 more
    Calycin (CL0116) MxiM; Pilot protein MxiM (PF11441; HMM-score: 14.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.095475
    • TAT(Tat/SPI): 0.003617
    • LIPO(Sec/SPII): 0.005535
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MRRLFSIGEALIDFIPNVTNANLKDVQTFTKQIGGAPCNVACTVQKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERDFSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGTVVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTGNVTVVIYTKGADGAAVYLKNGINHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLNLTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: ScrR (repression) regulon
    ScrR(TF)important in Sucrose utilization; RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]