PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2108 [new locus tag: NWMN_RS12200 ]
• pan locus tag^?: SAUPAN005620000
• symbol: NWMN_2108
• pan gene symbol^?: —
• synonym:
• product: HAD family hydrolase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2108 [new locus tag: NWMN_RS12200 ]
• symbol: NWMN_2108
• product: HAD family hydrolase
• replicon: chromosome
• strand: -
• coordinates: 2346156..2346971
• length: 816
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331291 NCBI
• RefSeq: YP_001333142 NCBI
• BioCyc:
• MicrobesOnline: 3707710 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  
  ATGTCAAAGCGTCTATTATTGTTTGATTTTGATGAAACATATTTTAAACATAATACAAAT
  GAAGAAGATTTAAGTCATTTAAGAGAAATGGAAAAATTGTTGGAAAAACTAACTAATAAT
  AATGAAGTGATCACTGCTGTATTGACAGGAAGTACATTTCAAAGTGTCATGGATAAAATG
  GATCAAGTTAATATGACGTTTAAACCATTACATATCTTTTCTGATTTGAGTTCTAAAATG
  TTCACTTGGAATAATGGCGAATATGTTGAATCAGAAACATATAAAAAGAAAGTCTTGAGC
  GAACCATTTTTATTTGAAGATATTGAAGATATATTACGTCATATTTCTGCGCAATATAAT
  GTCGAATTTATTCCACAAAGAGCATTTGAAGGTAATGAAACACATTATAATTTTTATTTT
  CATTCAACAGGTAATCACAACAATGATAGTCGTATCCTAGAAGCACTTGTCAGATACGCA
  AATGACCAAAACTATACAGCGAGATTTAGTCGAAGCAATCCATTAGCTGGTGATCCTGAA
  AATGCGTATGATATTGACTTCACACCGTCAAATGCTGGAAAATTATATGCTACTCAATTT
  CTGATGAGGAAATATAATATACCAGTAAAATCAATATTAGGTTTTGGAGATAGCGGCAAT
  GACGAAGCATATTTAAGCTATCTTGAACATGCATATTTGATGTCGAATAGTAGAGATGAA
  GCATTAAAGCAGAAATTTAGATTAACAAAATATCCATATTATCAAGGGATAACACTGCAT
  GTTAAAGAATTTGTTGAAGGTAAGTATGATTATTGA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  816
  

⊟Protein

⊟General

• locus tag: NWMN_2108 [new locus tag: NWMN_RS12200 ]
• symbol: NWMN_2108
• description: HAD family hydrolase
• length: 271
• theoretical pI: 5.21549
• theoretical MW: 31836.3
• GRAVY: -0.653875

⊟Function

• TIGRFAM:
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIB (TIGR01484; HMM-score: 175)
  and 9 more
  sucrose phosphatase (TIGR01485; EC 3.1.3.24; HMM-score: 44)
  Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 38.3)
  sucrose-phosphate synthase, sucrose phosphatase-like domain (TIGR02471; HMM-score: 29.6)
  sucrose-phosphate phosphatase subfamily (TIGR01482; EC 3.1.3.-; HMM-score: 27.9)
  Unknown function General mannosyl-3-phosphoglycerate phosphatase homolog (TIGR02463; EC 3.1.3.-; HMM-score: 16.1)
  mannosyl-3-phosphoglycerate phosphatase family (TIGR01486; EC 3.1.3.-; HMM-score: 15.4)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IB (TIGR01490; HMM-score: 14.6)
  Metabolism Amino acid biosynthesis Serine family phosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 11.8)
  phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 11.8)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  HAD (CL0137) S6PP; Sucrose-6F-phosphate phosphohydrolase (PF05116; HMM-score: 56.5)
  Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 51.4)
  and 3 more
  Trehalose_PPase; Trehalose-phosphatase (PF02358; HMM-score: 14.5)
  no clan defined DUF4974; Domain of unknown function (DUF4974) (PF16344; HMM-score: 12.8)
  HAD (CL0137) HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 8.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002111
  • TAT(Tat/SPI): 0.000157
  • LIPO(Sec/SPII): 0.000184
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222320 NCBI
• RefSeq: YP_001333142 NCBI
• UniProt: A0A0H3K9W5 UniProt

⊟Protein sequence

• MSKRLLLFDFDETYFKHNTNEEDLSHLREMEKLLEKLTNNNEVITAVLTGSTFQSVMDKMDQVNMTFKPLHIFSDLSSKMFTWNNGEYVESETYKKKVLSEPFLFEDIEDILRHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHNNDSRILEALVRYANDQNYTARFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMRKYNIPVKSILGFGDSGNDEAYLSYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYDY

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications