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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS12200 [old locus tag: NWMN_2108 ]
  • pan locus tag?: SAUPAN005620000
  • symbol: NWMN_RS12200
  • pan gene symbol?: —
  • synonym:
  • product: hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS12200 [old locus tag: NWMN_2108 ]
  • symbol: NWMN_RS12200
  • product: hydrolase
  • replicon: chromosome
  • strand: -
  • coordinates: 2346156..2346971
  • length: 816
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (2346156..2346971) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_2108

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGTCAAAGCGTCTATTATTGTTTGATTTTGATGAAACATATTTTAAACATAATACAAAT
    GAAGAAGATTTAAGTCATTTAAGAGAAATGGAAAAATTGTTGGAAAAACTAACTAATAAT
    AATGAAGTGATCACTGCTGTATTGACAGGAAGTACATTTCAAAGTGTCATGGATAAAATG
    GATCAAGTTAATATGACGTTTAAACCATTACATATCTTTTCTGATTTGAGTTCTAAAATG
    TTCACTTGGAATAATGGCGAATATGTTGAATCAGAAACATATAAAAAGAAAGTCTTGAGC
    GAACCATTTTTATTTGAAGATATTGAAGATATATTACGTCATATTTCTGCGCAATATAAT
    GTCGAATTTATTCCACAAAGAGCATTTGAAGGTAATGAAACACATTATAATTTTTATTTT
    CATTCAACAGGTAATCACAACAATGATAGTCGTATCCTAGAAGCACTTGTCAGATACGCA
    AATGACCAAAACTATACAGCGAGATTTAGTCGAAGCAATCCATTAGCTGGTGATCCTGAA
    AATGCGTATGATATTGACTTCACACCGTCAAATGCTGGAAAATTATATGCTACTCAATTT
    CTGATGAGGAAATATAATATACCAGTAAAATCAATATTAGGTTTTGGAGATAGCGGCAAT
    GACGAAGCATATTTAAGCTATCTTGAACATGCATATTTGATGTCGAATAGTAGAGATGAA
    GCATTAAAGCAGAAATTTAGATTAACAAAATATCCATATTATCAAGGGATAACACTGCAT
    GTTAAAGAATTTGTTGAAGGTAAGTATGATTATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    816

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS12200 [old locus tag: NWMN_2108 ]
  • symbol: NWMN_RS12200
  • description: hydrolase
  • length: 271
  • theoretical pI: 5.21549
  • theoretical MW: 31836.3
  • GRAVY: -0.653875

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIB (TIGR01484; HMM-score: 175)
    and 9 more
    sucrose phosphatase (TIGR01485; EC 3.1.3.24; HMM-score: 44)
    Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 38.3)
    sucrose-phosphate synthase, sucrose phosphatase-like domain (TIGR02471; HMM-score: 29.6)
    sucrose-phosphate phosphatase subfamily (TIGR01482; EC 3.1.3.-; HMM-score: 27.9)
    Unknown function General mannosyl-3-phosphoglycerate phosphatase homolog (TIGR02463; EC 3.1.3.-; HMM-score: 16.1)
    mannosyl-3-phosphoglycerate phosphatase family (TIGR01486; EC 3.1.3.-; HMM-score: 15.4)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IB (TIGR01490; HMM-score: 14.6)
    Metabolism Amino acid biosynthesis Serine family phosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 11.8)
    phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 11.8)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    HAD (CL0137) S6PP; Sucrose-6F-phosphate phosphohydrolase (PF05116; HMM-score: 56.5)
    Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 51.4)
    and 3 more
    Trehalose_PPase; Trehalose-phosphatase (PF02358; HMM-score: 14.5)
    no clan defined DUF4974; Domain of unknown function (DUF4974) (PF16344; HMM-score: 12.8)
    HAD (CL0137) HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 8.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002111
    • TAT(Tat/SPI): 0.000157
    • LIPO(Sec/SPII): 0.000184
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKRLLLFDFDETYFKHNTNEEDLSHLREMEKLLEKLTNNNEVITAVLTGSTFQSVMDKMDQVNMTFKPLHIFSDLSSKMFTWNNGEYVESETYKKKVLSEPFLFEDIEDILRHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHNNDSRILEALVRYANDQNYTARFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMRKYNIPVKSILGFGDSGNDEAYLSYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYDY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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