PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2264 [new locus tag: NWMN_RS13035 ]
• pan locus tag^?: SAUPAN005871000
• symbol: NWMN_2264
• pan gene symbol^?: hssS
• synonym:
• product: sensor histidine kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2264 [new locus tag: NWMN_RS13035 ]
• symbol: NWMN_2264
• product: sensor histidine kinase
• replicon: chromosome
• strand: +
• coordinates: 2486491..2487864
• length: 1374
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331379 NCBI
• RefSeq: YP_001333298 NCBI
• BioCyc:
• MicrobesOnline: 3707866 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGTTTAAAACACTCTATGCTAGAATTGCGATTTATTCCATTACGGTTATTTTATTTAGT
  GCATTAATAAGTTTTGTATTAACAAATGTTTACTATCATTATAATTTAAAAGCATCTAAT
  GACGCGAAAATCATGAAGACGCTTAAAGAAGCAAGACAATATGAACAATCAGCTAAACCA
  ACGCACATTCAACAATATTTCAAACATTTAGGGCAAATGAATTACCAAATAATGACCATT
  GATCAAAAAGGTCATAAGACATTTTATGGGGAGCCATTTAGAGAAGATACTTTATCACAA
  AATGCTATTAACAACGTATTAAATAATCAAGATTACCATGGTATTAAAGATAAACCATTC
  GCATTATTCGTAACTGGATTTTTCGACAATGTAACAGATAATACTGTTGGTATTAACTTT
  AAGACAAAAGACGGTTCAATAGCAGTATTTATGCGTCCAGATATTGGAGAAACATTTAGC
  GAGTTTAGAACATTCTTAGCCGTCTTGTTAATGTTGTTATTATTTATCTCGATTTCATTA
  GTTATCGCATCAACCTATTCAATCATTCGTCCAGTAAAAAAGTTAAAGCTAGCGACCGAA
  CGCTTAATTGATGGTGATTTTGAAACACCTATCAAACAAACACGCAAAGATGAAATTGGA
  ACATTACAGTATCACTTTAATAAGATGAGAGAGTCATTGGGACAAGTTGATCAAATGAGG
  CAGCATTTTGTACAAAATGTATCTCATGAAATCAAAACACCATTAACACATATTCATCAT
  TTATTAAGCGAGTTACAACAAACTTCTGATAAAACATTAAGACAACAATATATAAATGAT
  ATCTATACCATTACAACGCAGCTGAGCGGATTAACAACTGAATTGTTGCTCTTATCCGAA
  TTAGATAATCACCAACATTTATTATTCGATGATAAGATTCAAGTCAATCAACTCATTAAA
  GATATCATTCGACATGAACAATTTGCTGCTGATGAAAAGTCGTTAATCATTTTAGCTGAC
  CTAGAATCAATCAATTTCCTAGGAAATCAACGTTTGTTACATCAAGCATTGAGTAATTTA
  TTAATAAATGCTATTAAGTATACAGATGTTGGTGGTGCCATTGACATCGCTTTGCAACAT
  AGTCACAACAATATCATTTTCACAATAAGTAATGATGGTTCACCAATTAGTCCACAAGCT
  GAAGCACGTTTATTTGAACGTTTTTATAAAGTGAGCAAACATGATAATAGCAATGGTTTA
  GGTCTTGCAATTACAAAATCCATCATTGAATTGCATCATGGTACAATCCAATTCACACAA
  AGTAACGAGTATGTAACCACCTTTACTATTACACTGCCAAATAATTCACTTTAA
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⊟Protein

⊟General

• locus tag: NWMN_2264 [new locus tag: NWMN_RS13035 ]
• symbol: NWMN_2264
• description: sensor histidine kinase
• length: 457
• theoretical pI: 7.2453
• theoretical MW: 52374.5
• GRAVY: -0.181838

⊟Function

• reaction:
  EC 2.7.13.3?  ExPASy
  Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
• TIGRFAM:
  Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 158.1)
  heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 151.2)
  and 6 more
  proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 106.5)
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 86.8)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 47.9)
  Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 47.9)
  Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 19.1)
  Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 19.1)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 71)
  and 8 more
  no clan defined HAMP; HAMP domain (PF00672; HMM-score: 36.2)
  His_Kinase_A (CL0025) HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 33.6)
  HATPase_c_5; GHKL domain (PF14501; HMM-score: 28.3)
  HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 15)
  no clan defined Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 14.4)
  Blo-t-5; Mite allergen Blo t 5 (PF11642; HMM-score: 14.1)
  DUF4199; Protein of unknown function (DUF4199) (PF13858; HMM-score: 11.6)
  SAPIS-gp6; Pathogenicity island protein gp6 in Staphylococcus (PF16722; HMM-score: 9.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.01
  • Cytoplasmic Membrane Score: 9.99
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 2
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.09055
  • TAT(Tat/SPI): 0.000758
  • LIPO(Sec/SPII): 0.027154
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: 151222476 NCBI
• RefSeq: YP_001333298 NCBI
• UniProt: A6QJK4 UniProt

⊟Protein sequence

• MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKIMKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTIDQKGHKTFYGEPFREDTLSQNAINNVLNNQDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFSEFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDEIGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQYINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVNQLIKDIIRHEQFAADEKSLIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISNDGSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTITLPNNSL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_2263 > NWMN_2264

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications