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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL2359 [new locus tag: SACOL_RS12390 ]
- pan locus tag?: SAUPAN005871000
- symbol: SACOL2359
- pan gene symbol?: hssS
- synonym:
- product: sensor histidine kinase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL2359 [new locus tag: SACOL_RS12390 ]
- symbol: SACOL2359
- product: sensor histidine kinase
- replicon: chromosome
- strand: +
- coordinates: 2417990..2419363
- length: 1374
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3238332 NCBI
- RefSeq: YP_187164 NCBI
- BioCyc: see SACOL_RS12390
- MicrobesOnline: 913840 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1321ATGTTTAAAACACTCTATGCTAGAATTGCGATTTATTCCATTACGGTTATTTTATTTAGT
GCATTAATAAGTTTTGTATTAACAAATGTTTACTATCATTATAATTTAAAAGCATCTAAT
GACGCGAAAATCATGAAGACGCTTAAAGAAGCAAGACAATATGAACAATCAGCTAAACCA
ACGCACATTCAACAATATTTCAAACATTTAGGGCAAATGAATTACCAAATAATGACCATT
GATCAAAAAGGTCATAAGACATTTTATGGGGAGCCATTTAGAGAAGATACTTTATCACAA
AATGCTATTAACAACGTATTAAATAATCAAGATTACCATGGTATTAAAGATAAACCATTC
GCATTATTCGTAACTGGATTTTTCGACAATGTAACAGATAATACTGTTGGTATTAACTTT
AAGACAAAAGACGGTTCAATAGCAGTATTTATGCGTCCAGATATTGGAGAAACATTTAGC
GAGTTTAGAACATTCTTAGCCGTCTTGTTAATGTTGTTATTATTTATCTCGATTTCATTA
GTTATCGCATCAACCTATTCAATCATTCGTCCAGTAAAAAAGTTAAAGCTAGCGACCGAA
CGCTTAATTGATGGTGATTTTGAAACACCTATCAAACAAACACGCAAAGATGAAATTGGA
ACATTACAGTATCACTTTAATAAGATGAGAGAGTCATTGGGACAAGTTGATCAAATGAGG
CAGCATTTTGTACAAAATGTATCTCATGAAATCAAAACACCATTAACACATATTCATCAT
TTATTAAGCGAGTTACAACAAACTTCTGATAAAACATTAAGACAACAATATATAAATGAT
ATCTATACCATTACAACGCAGCTGAGCGGATTAACAACTGAATTGTTGCTCTTATCCGAA
TTAGATAATCACCAACATTTATTATTCGATGATAAGATTCAAGTCAATCAACTCATTAAA
GATATCATTCGACATGAACAATTTGCTGCTGATGAAAAGTCGTTAATCATTTTAGCTGAC
CTAGAATCAATCAATTTCCTAGGAAATCAACGTTTGTTACATCAAGCATTGAGTAATTTA
TTAATAAATGCTATTAAGTATACAGATGTTGGTGGTGCCATTGACATCGCTTTGCAACAT
AGTCACAACAATATCATTTTCACAATAAGTAATGATGGTTCACCAATTAGTCCACAAGCT
GAAGCACGTTTATTTGAACGTTTTTATAAAGTGAGCAAACATGATAATAGCAATGGTTTA
GGTCTTGCAATTACAAAATCCATCATTGAATTGCATCATGGTACAATCCAATTCACACAA
AGTAACGAGTATGTAACCACCTTTACTATTACACTGCCAAATAATTCACTTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL2359 [new locus tag: SACOL_RS12390 ]
- symbol: SACOL2359
- description: sensor histidine kinase
- length: 457
- theoretical pI: 7.2453
- theoretical MW: 52374.5
- GRAVY: -0.181838
⊟Function[edit | edit source]
- reaction: EC 2.7.13.3? ExPASyHistidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
- TIGRFAM: Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 158.1)heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 151.2)and 6 moreproteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 106.5)Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 86.8)Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 47.9)Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 47.9)Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 19.1)Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 19.1)
- TheSEED :
- Sensor histidine kinase colocalized with HrtAB transporter
- PFAM: His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 71)and 8 moreno clan defined HAMP; HAMP domain (PF00672; HMM-score: 36.2)His_Kinase_A (CL0025) HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 33.6)HATPase_c_5; GHKL domain (PF14501; HMM-score: 28.3)HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 15)no clan defined Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 14.4)Blo-t-5; Mite allergen Blo t 5 (PF11642; HMM-score: 14.1)DUF4199; Protein of unknown function (DUF4199) (PF13858; HMM-score: 11.6)SAPIS-gp6; Pathogenicity island protein gp6 in Staphylococcus (PF16722; HMM-score: 9.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0.01
- Cytoplasmic Membrane Score: 9.99
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 2
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -0.5
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.09055
- TAT(Tat/SPI): 0.000758
- LIPO(Sec/SPII): 0.027154
- predicted transmembrane helices (TMHMM): 2
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKIMKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTIDQKGHKTFYGEPFREDTLSQNAINNVLNNQDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFSEFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDEIGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQYINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVNQLIKDIIRHEQFAADEKSLIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISNDGSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTITLPNNSL
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Integral membrane [1] [2]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)