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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2369
  • pan locus tag?: SAUPAN006035000
  • symbol: NWMN_2369
  • pan gene symbol?: —
  • synonym:
  • product: short chain dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2369
  • symbol: NWMN_2369
  • product: short chain dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 2606868..2607506
  • length: 639
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    GTGGATAATGCAAGCGAACAACAAGTGGTAGACTTTGTGTCTGACATAAAGGAACAGTTT
    GGAAGAATCGATGTGTTGTTTAATAATGCCGGTGTGGATAATGCGGCTGATAGAATTCAT
    GAGTATCCAATAGATGTGTATGACAAGATTATGAATGTAGATATGCGTGGGACATTTTTA
    ATGACGAAAATGATGTTACCTTTAATGATGAATCAAGGTGGCTCTATTGTTAATACGTCT
    TCATTTTCCGGACAAGCAGCAGACTTGTATCGCTCTGGATATAATGCTGCGAAAGGTGCA
    GTGATTAATTTTACAAAATCAATCGCAATTGAGTATGGCCGTGATAGCATTCGAGCCAAT
    GCGATTGCACCAGGTACAATTGAAACCCCGTTAGTAGATAAACTGACAGGTACGAGTGAG
    GATGATGCAGGTAAAACATTTAGAGAAAATCAAAAATGGATGACTCCGCTGGGACGTTTA
    GGTAAACCAGAAGAAGTTGCTAAATTAGTAGTCTTCTTAGCATCTGACGACAGTTCATTC
    ATAACTGGAGAGACGATTCGAATTGATGGTGGTGTGATGGCTTACACATGGCCTGGCGAG
    ATGTTAAGTGATGATTCGTGGAAGCGGACATTGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    639

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2369
  • symbol: NWMN_2369
  • description: short chain dehydrogenase
  • length: 212
  • theoretical pI: 4.37938
  • theoretical MW: 23385.3
  • GRAVY: -0.270755

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase (TIGR01830; EC 1.1.1.100; HMM-score: 153.3)
    Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR03971; EC 1.1.99.-; HMM-score: 142.1)
    3-hydroxybutyrate dehydrogenase (TIGR01963; HMM-score: 141.9)
    acetoacetyl-CoA reductase (TIGR01829; EC 1.1.1.36; HMM-score: 129.8)
    and 10 more
    Metabolism Energy metabolism Fermentation acetoin reductases (TIGR02415; EC 1.1.1.-; HMM-score: 117)
    2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (TIGR03206; EC 1.1.1.-; HMM-score: 116.9)
    2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (TIGR04316; EC 1.3.1.28; HMM-score: 114.2)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 2-deoxy-D-gluconate 3-dehydrogenase (TIGR01832; EC 1.1.1.125; HMM-score: 112.3)
    Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR04504; EC 1.1.99.-; HMM-score: 92.3)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis putative 3-oxoacyl-(acyl-carrier-protein) reductase (TIGR01831; HMM-score: 81.9)
    rhamnulose-1-phosphate aldolase/alcohol dehydrogenase (TIGR02632; EC 1.1.1.1,4.1.2.19; HMM-score: 70.3)
    cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (TIGR03325; EC 1.3.1.56; HMM-score: 66.2)
    pteridine reductase (TIGR02685; EC 1.5.1.33; HMM-score: 53.1)
    sepiapterin reductase (TIGR01500; EC 1.1.1.153; HMM-score: 19)
  • TheSEED: data available for N315, NCTC8325, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 190.9)
    and 2 more
    adh_short; short chain dehydrogenase (PF00106; HMM-score: 122.9)
    KR; KR domain (PF08659; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00474
    • TAT(Tat/SPI): 0.000387
    • LIPO(Sec/SPII): 0.000888
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDNASEQQVVDFVSDIKEQFGRIDVLFNNAGVDNAADRIHEYPIDVYDKIMNVDMRGTFLMTKMMLPLMMNQGGSIVNTSSFSGQAADLYRSGYNAAKGAVINFTKSIAIEYGRDSIRANAIAPGTIETPLVDKLTGTSEDDAGKTFRENQKWMTPLGRLGKPEEVAKLVVFLASDDSSFITGETIRIDGGVMAYTWPGEMLSDDSWKRTLE

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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